{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:29:41Z","timestamp":1769059781218,"version":"3.49.0"},"reference-count":96,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Ant\u00e1rtico Chileno (INACH) Project","award":["RT_16_20"],"award-info":[{"award-number":["RT_16_20"]}]},{"name":"ANID","award":["RT_16_20"],"award-info":[{"award-number":["RT_16_20"]}]},{"name":"INACH\/Chile","award":["RT_16_20"],"award-info":[{"award-number":["RT_16_20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Antarctic soil represents an important reservoir of filamentous fungi (FF) species with the ability to produce novel bioactive lipids. However, the lipid extraction method is still a bottleneck. The objective of the present work was to isolate and identify cultivable FF from Antarctic soils, to assess the most effective methods for fatty acid (FA) extraction, and to characterise the obtained lipids. A total of 18 fungal strains belonging to the Botrytis, Cladosporium, Cylindrobasidium, Mortierella, Penicillium, Pseudogymnoascus, and Talaromyces genera and the Melanommataceae family were isolated and identified. The Folch, Bligh and Dyer, and Lewis extraction methods were assessed, and methyl esters of FA (FAMEs) were obtained. The Lewis method was the best in recovering FAMEs from fungal biomass. A total of 17 FAs were identified, and their chemical compositions varied depending on fungal species and strain. Oleic, linoleic, stearic, and palmitic acids were predominant for all fungal strains in the three assessed methods. Among the analysed strains, Cylindrobasidium eucalypti, Penicillium miczynskii, P. virgatum, and Pseudogymnoascus pannorum produced high amounts of FA. This suggests that the soils of Antarctica Bay, as well as harbouring known oleaginous fungi, are also an important source of oleaginous filamentous fungi that remain poorly analysed.<\/jats:p>","DOI":"10.3390\/microorganisms13030504","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T03:40:12Z","timestamp":1740454812000},"page":"504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lipid Production in Cultivable Filamentous Fungi Isolated from Antarctic Soils: A Comprehensive Study"],"prefix":"10.3390","volume":"13","author":[{"given":"Victor","family":"Gallardo","sequence":"first","affiliation":[{"name":"Doctoral Program in Science of Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Postgraduate Program in Biotechnology, Federal University of Technology-Paran\u00e1, Ponta Grossa 84017-220, Brazil"}]},{"given":"J\u00e9ssica","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Instituto de Ci\u00eancias Biol\u00f3gicas\u2014ICB, Universidade Federal do Amazonas, Manaus 69080-900, Brazil"},{"name":"Department of Chemical Science and Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6837-4559","authenticated-orcid":false,"given":"Marcela","family":"Sep\u00falveda","sequence":"additional","affiliation":[{"name":"Doctoral Program in Science of Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Postgraduate Program in Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba 80060-000, Brazil"}]},{"given":"Yasna","family":"Cay\u00fan","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2514-4583","authenticated-orcid":false,"given":"Christian","family":"Santander","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-3438","authenticated-orcid":false,"given":"Excequel","family":"Ponce","sequence":"additional","affiliation":[{"name":"Escuela de Agronom\u00eda, Facultad de Ciencias Agron\u00f3micas y de los Alimentos, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Quillota 2340025, Chile"}]},{"given":"Juliana","family":"Bittencourt","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biotechnology, Federal University of Technology-Paran\u00e1, Ponta Grossa 84017-220, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8549-6302","authenticated-orcid":false,"given":"C\u00e9sar","family":"Arriagada","sequence":"additional","affiliation":[{"name":"Laboratorio Biorremediaci\u00f3n, Departamento de Ciencias Forestales, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile"}]},{"given":"Javiera","family":"Soto","sequence":"additional","affiliation":[{"name":"Laboratorio Biorremediaci\u00f3n, Departamento de Ciencias Forestales, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9927-2919","authenticated-orcid":false,"given":"Romina","family":"Pedreschi","sequence":"additional","affiliation":[{"name":"Escuela de Agronom\u00eda, Facultad de Ciencias Agron\u00f3micas y de los Alimentos, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Quillota 2340025, Chile"},{"name":"Millennium Institute Center for Genome Regulation (CRG), Santiago 8331150, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2953-4861","authenticated-orcid":false,"given":"Vania Aparecida","family":"Vicente","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biotechnology, Federal University of Technology-Paran\u00e1, Ponta Grossa 84017-220, Brazil"},{"name":"Postgraduate Program in Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba 80060-000, Brazil"},{"name":"Postgraduate Program in Bioprocess Engineering and Biotechnology, Federal University of Parana, Curitiba 84017-220, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2124-3100","authenticated-orcid":false,"given":"Pablo","family":"Cornejo","sequence":"additional","affiliation":[{"name":"Plant Stress Physiology Laboratory, Centro de Estudios Avanzados en Fruticultura (CEAF), Rengo 2940000, Chile"},{"name":"Centro Tecnol\u00f3gico de Suelos y Cultivos (CTSyC), Facultad de Ciencias Agrarias, Universidad de Talca, Talca 3460000, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-0941","authenticated-orcid":false,"given":"Cledir","family":"Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biotechnology, Federal University of Technology-Paran\u00e1, Ponta Grossa 84017-220, Brazil"},{"name":"Department of Chemical Science and Natural Resources, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Centro Regional de Investigaci\u00f3n e Innovaci\u00f3n para la Sostenibilidad de la Agricultura y los Territorios Rurales, CERES, La Palma, Quillota 2260000, Chile"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Stan-Lotter, H., and Fendrihan, S. 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