{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T09:53:45Z","timestamp":1771062825286,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SelecTEcoli","award":["ALT20-03-0145-FEDER-000034"],"award-info":[{"award-number":["ALT20-03-0145-FEDER-000034"]}]},{"name":"SelecTEcoli","award":["ALT20-05-3559-ESF-000076"],"award-info":[{"award-number":["ALT20-05-3559-ESF-000076"]}]},{"name":"SelecTEcoli","award":["UIDB\/05183"],"award-info":[{"award-number":["UIDB\/05183"]}]},{"name":"SelecTEcoli","award":["LA\/P\/0121\/2020"],"award-info":[{"award-number":["LA\/P\/0121\/2020"]}]},{"name":"Alentejo 2020","award":["ALT20-03-0145-FEDER-000034"],"award-info":[{"award-number":["ALT20-03-0145-FEDER-000034"]}]},{"name":"Alentejo 2020","award":["ALT20-05-3559-ESF-000076"],"award-info":[{"award-number":["ALT20-05-3559-ESF-000076"]}]},{"name":"Alentejo 2020","award":["UIDB\/05183"],"award-info":[{"award-number":["UIDB\/05183"]}]},{"name":"Alentejo 2020","award":["LA\/P\/0121\/2020"],"award-info":[{"award-number":["LA\/P\/0121\/2020"]}]},{"name":"Foundation for Science and Technology of Portugal (FCT)","award":["ALT20-03-0145-FEDER-000034"],"award-info":[{"award-number":["ALT20-03-0145-FEDER-000034"]}]},{"name":"Foundation for Science and Technology of Portugal (FCT)","award":["ALT20-05-3559-ESF-000076"],"award-info":[{"award-number":["ALT20-05-3559-ESF-000076"]}]},{"name":"Foundation for Science and Technology of Portugal (FCT)","award":["UIDB\/05183"],"award-info":[{"award-number":["UIDB\/05183"]}]},{"name":"Foundation for Science and Technology of Portugal (FCT)","award":["LA\/P\/0121\/2020"],"award-info":[{"award-number":["LA\/P\/0121\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Concerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of Escherichia coli were subjected to various acid concentrations. Samples were taken during fermentation to monitor growth, sugar consumption, biomass yield, and product yield. With increasing AI, the TG1 strain maintained stable growth (0.102 1\/h), while xylose consumption decreased, and product formation improved, making it better suited for high-acetic-acid industrial applications. In contrast, the Tuner strain performed better under low-inhibitor conditions but suffered metabolic inhibition at high AI levels, compensating by increasing lactic acid production\u2014an adaptation absent in TG1. However, Tuner showed greater resistance to formic acid stress, sustaining higher growth and ethanol production, whereas TG1 experienced a greater metabolic decline but maintained stable acetic acid output. Both strains experienced inhibition in formic acid metabolism, but TG1 had a higher yield despite its lower overall robustness in formic acid conditions. The use of TG1 for value-added compounds such as ethanol or formic acid may help to avoid the use of chemicals that eliminate acetic acid. Tuner could be used for lactic acid production, especially in hydrolysates with under moderate concentration.<\/jats:p>","DOI":"10.3390\/microorganisms13030605","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T09:37:52Z","timestamp":1741167472000},"page":"605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of Escherichia coli"],"prefix":"10.3390","volume":"13","author":[{"given":"Suelen S.","family":"Gaspar","sequence":"first","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1431-4188","authenticated-orcid":false,"given":"J\u00fania","family":"Alves-Ferreira","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"},{"name":"Forest Research Centre, TERRA Associate Laboratory, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"given":"Patr\u00edcia","family":"Moniz","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"}]},{"given":"Talita","family":"Silva-Fernandes","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"}]},{"given":"Adriana I. R.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"}]},{"given":"Ivone","family":"Torrado","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"},{"name":"Forest Research Centre, TERRA Associate Laboratory, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal"},{"name":"LNEG\u2014National Laboratory of Energy and Geology, Bioenergy and Biorefineries Unit, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0768-8162","authenticated-orcid":false,"given":"Gaetano R.","family":"Pesce","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padua, Agripolis Campus, Viale dell\u2019Universit\u00e0 16, 35020 Legnaro, PD, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-4832","authenticated-orcid":false,"given":"Florbela","family":"Carvalheiro","sequence":"additional","affiliation":[{"name":"LNEG\u2014National Laboratory of Energy and Geology, Bioenergy and Biorefineries Unit, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5820-3669","authenticated-orcid":false,"given":"Lu\u00eds C.","family":"Duarte","sequence":"additional","affiliation":[{"name":"LNEG\u2014National Laboratory of Energy and Geology, Bioenergy and Biorefineries Unit, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1977-8771","authenticated-orcid":false,"given":"Maria C.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)\/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, CEBAL-Alentejo Biotechnology Center for Agriculture and Agro-Food, Apartado 6158, 7801-908 Beja, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/nature07190","article-title":"Genomics of cellulosic biofuels","volume":"454","author":"Rubin","year":"2008","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1016\/j.biortech.2009.10.079","article-title":"Bioethanol production from rice straw: An overview","volume":"101","author":"Binod","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.biortech.2015.03.037","article-title":"Enzymatic sac-charification and bioethanol production from Cynara cardunculus pretreated by steam explosion","volume":"186","author":"Fernandes","year":"2015","journal-title":"Bioresour. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Brosowski, A., Krause, T., Mantau, U., Mahro, B., Noke, A., Richter, F., Raussen, T., Bischof, R., Hering, T., and Blanke, C. (2019). How to measure the impact of biogenic residues, wastes and by-products: Development of a national re-source monitoring based on the example of Germany. Biomass Bioenergy, 127.","DOI":"10.1016\/j.biombioe.2019.105275"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s10295-007-0293-6","article-title":"Reviving the carbohydrate economy via multi-product lignocellulose biorefineries","volume":"35","author":"Zhang","year":"2008","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110691","DOI":"10.1016\/j.rser.2020.110691","article-title":"Biomass for a sustainable bioeconomy: An overview of world biomass production and utilization","volume":"139","author":"Antar","year":"2021","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_7","first-page":"849","article-title":"Hemicellulose biorefineries: A review on biomass pretreatments","volume":"67","author":"Carvalheiro","year":"2008","journal-title":"J. Sci. Ind. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1515\/bioeth-2016-0007","article-title":"Bioethanol production from extracted olive pomace: Dilute acid hydrolysis","volume":"2","author":"Fernandes","year":"2016","journal-title":"Bioethanol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.bej.2015.04.009","article-title":"Bioethanol production from steam explosion pretreated and alkali extracted Cistus ladanifer (rockrose)","volume":"104","author":"Ferro","year":"2015","journal-title":"Biochem. Eng. J."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"G\u00edrio, F.M., Carvalheiro, F., and Duarte, L.C.B.-L.R. (2012). Deconstruction of the Hemicellulose Fraction from Lignocellulosic Materials into Simple Sugars, Springer. D-Xylitol\u2014Fermentative Production, Application and Commercialization.","DOI":"10.1007\/978-3-642-31887-0_1"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.bej.2013.02.002","article-title":"The simultaneous utilization of kinetic analysis and flow cytometry in the assessment of Lactobacillus rhamnosus ATCC 7469 physiological states produced by increasing oxygen limitation levels and lactic acid accumulation","volume":"74","author":"Marques","year":"2013","journal-title":"Biochem. Eng. J."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wu, Y., Shen, X., Yuan, Q., and Yan, Y. (2016). Metabolic Engineering Strategies for Co-Utilization of Carbon Sources in Microbes. Bioengineering, 3.","DOI":"10.3390\/bioengineering3010010"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.biotechadv.2015.01.008","article-title":"Cell disruption for microalgae biorefineries","volume":"33","author":"Eppink","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Dixon, C., and Wilken, L.R. (2018). Green microalgae biomolecule separations and recovery. Bioresour. Bioprocess., 5.","DOI":"10.1186\/s40643-018-0199-3"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1007\/s12155-012-9208-0","article-title":"Pretreatment and Lignocellulosic Chemistry","volume":"5","author":"Hu","year":"2012","journal-title":"BioEnergy Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kim, D. (2018). Physico-chemical conversion of lignocellulose: Inhibitor effects and detoxification strategies: A mini review. Molecules, 23.","DOI":"10.3390\/molecules23020309"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s10295-008-0327-8","article-title":"Bioconversion of lignocellulosic biomass: Biochemical and molecular perspectives","volume":"35","author":"Kumar","year":"2008","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.biombioe.2016.05.001","article-title":"Fractional pretreatment of raw and calcium oxalate-extracted agave bagasse using ionic liquid and alkaline hydrogen peroxide","volume":"91","author":"Stavila","year":"2016","journal-title":"Biomass Bioenergy"},{"key":"ref_19","first-page":"339","article-title":"Dilute sulfuric acid pretreatment of agricultural and agro-industrial residues for ethanol production","volume":"137\u2013140","author":"Martin","year":"2007","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.rser.2019.01.048","article-title":"Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production","volume":"105","author":"Zabed","year":"2019","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/BF01870573","article-title":"Monocarboxylic acid permeation through lipid bilayer membranes","volume":"77","author":"Walter","year":"1984","journal-title":"J. Membr. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1385\/ABAB:129:1:278","article-title":"Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis","volume":"129","author":"Ballesteros","year":"2006","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/(SICI)1097-0290(1999)66:4<203::AID-BIT1>3.0.CO;2-#","article-title":"Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01","volume":"66","author":"Zaldivar","year":"1999","journal-title":"Biotechnol. Bioeng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1754-6834-6-16","article-title":"Bioconversion of lignocellulose: Inhibitors and detoxification","volume":"6","author":"Alriksson","year":"2013","journal-title":"Biotechnol. Biofuels"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.biortech.2015.10.009","article-title":"Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects","volume":"199","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16885","DOI":"10.1016\/j.ijhydene.2014.08.063","article-title":"Effects of 5-hydromethylfurfural, levulinic acid and formic acid, pretreatment byproducts of biomass, on fermentative H2 production from glucose and galactose","volume":"39","author":"Kumar","year":"2014","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1016\/j.biotechadv.2014.04.007","article-title":"Do furanic and phenolic compounds of lignocellulosic and algae biomass hydrolyzate inhibit anaerobic mixed cultures? A comprehensive review","volume":"32","author":"Monlau","year":"2014","journal-title":"Biotechnol. Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1754-6834-1-8","article-title":"Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities","volume":"1","author":"Flores","year":"2008","journal-title":"Biotechnol. Biofuels"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1021\/bp9901062","article-title":"Enteric bacterial catalysts for fuel ethanol production","volume":"15","author":"Ingram","year":"1999","journal-title":"Biotechnol. Prog."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.5483\/BMBRep.2012.45.2.59","article-title":"Rewiring carbon catabolite repression for microbial cell factory","volume":"45","author":"Vinuselvi","year":"2012","journal-title":"BMB Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1007\/s00253-012-4177-y","article-title":"Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium","volume":"96","author":"Bustos","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s00253-004-1642-2","article-title":"Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass","volume":"66","author":"Klinke","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.ymben.2010.03.004","article-title":"A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: From biofuels and chemicals, to biocatalysis and bioremediation","volume":"12","author":"Nicolaou","year":"2010","journal-title":"Metab. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Li, Z., Huang, Z., and Gu, P. (2024). Response of Escherichia coli to Acid Stress: Mechanisms and Applications\u2014A Narrative Review. Microorganisms, 12.","DOI":"10.3390\/microorganisms12091774"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1038\/s41467-024-45448-z","article-title":"Systems engineering of Escherichia coli for high-level glutarate production from glucose","volume":"15","author":"Zhang","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Elbing, K., and Brent, R. (2002). Media Preparation and Bacteriological Tools. Curr. Protoc. Mol. Biol., 59.","DOI":"10.1002\/0471142727.mb0101s59"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s10529-006-9252-y","article-title":"Low salt medium for lactate and ethanol production by recombinant Escherichia coli B","volume":"29","author":"Martinez","year":"2006","journal-title":"Biotechnol. Lett."},{"key":"ref_38","unstructured":"Lennete, E.H., Balos, A., and Hausler, W.J. (1980). Susceptibility Testing with Antifungal Drugs. Manual of Clinical Microbiology, American Society for Microbiology. [3rd ed.]."},{"key":"ref_39","unstructured":"Ver\u00edssimo, A., and Morais, P. (2024, December 02). MICROBIOLOGIA\u2014Medi\u00e7\u00e3o do Crescimento de E. Coli. Departamento de Zoologia da Universidade de Coimbra. pp. 1-5. Available online: https:\/\/silo.tips\/download\/departamento-de-zoologia-da-universidade-de-coimbra-8."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"114519","DOI":"10.1016\/j.indcrop.2022.114519","article-title":"D-Lactic acid production from Cistus ladanifer residues: Co-fermentation of pentoses and hexoses by Escherichia coli JU15","volume":"177","author":"Carvalheiro","year":"2022","journal-title":"Ind. Crops Prod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.bej.2018.09.015","article-title":"Technical and economic potential evaluation of the strain Escherichia coli MS04 in the ethanol production from glucose and xylose","volume":"140","author":"Martinez","year":"2018","journal-title":"Biochem. Eng. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.biortech.2016.08.067","article-title":"Production of D-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered Escherichia coli strains","volume":"220","author":"Utrilla","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ymben.2012.07.007","article-title":"Engineering and adaptive evolution of Escherichia coli for d-lactate fermentation reveals GatC as a xylose transporter","volume":"14","author":"Utrilla","year":"2012","journal-title":"Metab. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"90","DOI":"10.5004\/dwt.2011.2063","article-title":"Removal of inhibitory compounds from olive stone auto-hydrolysis liquors by nanofiltration","volume":"27","author":"Fernandes","year":"2011","journal-title":"Desalination Water Treat."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.biortech.2009.08.091","article-title":"Acid hydrolysis of sugarcane bagasse for lactic acid production","volume":"101","author":"Laopaiboon","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0960-8524(98)00037-6","article-title":"Biotechnological production of xylitol. Part 3: Operation in culture media made from lignocellulose hydrolysates","volume":"66","year":"1998","journal-title":"Bioresour. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/BF02920121","article-title":"Comparison of yellow poplar pretreatment between NREL digester and sunds hydrolyzer","volume":"70\u201372","author":"Tucker","year":"1998","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1128\/aem.56.4.1004-1011.1990","article-title":"Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations","volume":"56","author":"Luli","year":"1990","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1128\/JB.180.4.767-772.1998","article-title":"Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acids","volume":"180","author":"Roe","year":"1998","journal-title":"J. Bacteriol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/sj.jim.2900543","article-title":"Comparison of aromatic monomers in lignocellulosic biomass prehydrolysates","volume":"20","author":"Fenske","year":"1998","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/s00253-003-1444-y","article-title":"Bacteria engineered for fuel ethanol production: Current status","volume":"63","author":"Dien","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.biortech.2015.09.036","article-title":"Non-severe thermochemical hydrolysis of stover from white corn and sequential enzymatic saccharification and fermentation to ethanol","volume":"198","author":"Tiessen","year":"2015","journal-title":"Bioresour. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0032-9592(95)85008-2","article-title":"The effect of glucose concentration on the growth rate and some intracellular components of a recombinant E. coli culture","volume":"30","author":"Kazan","year":"1995","journal-title":"Process. Biochem."},{"key":"ref_54","first-page":"86","article-title":"Lactic acid production from glucose and xylose using the lactogenic Escherichia coli strain JU15: Experiments and techno-economic results","volume":"273","author":"Martinez","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1159\/000111993","article-title":"Specific ethanol production rate in ethanologenic Escherichia coli Strain KO11 is limited by pyruvate decarboxylase","volume":"15","author":"Utrilla","year":"2008","journal-title":"Microb. Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1385\/ABAB:121:1-3:0413","article-title":"Effects of Aliphatic Acids, Furfural, and Phenolic Compounds on Debaryomyces hansenii CCMI 941","volume":"121\u2013124","author":"Duarte","year":"2005","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1002\/(SICI)1097-0290(20000605)68:5<524::AID-BIT6>3.0.CO;2-T","article-title":"Effect of alcohol compounds found in hemicellulose hydrolysate on the growth and fermentation of ethanologenic Escherichia coli","volume":"68","author":"Zaldivar","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.bej.2014.05.011","article-title":"Aeration-enhanced bioethanol production","volume":"92","author":"Deniz","year":"2014","journal-title":"Biochem. Eng. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.apenergy.2013.07.036","article-title":"Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol","volume":"113","author":"Caspeta","year":"2014","journal-title":"Appl. Energy"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jbiosc.2014.07.007","article-title":"Fed-batch fermentation for enhanced lactic acid production from glucose\/xylose mixture without carbon catabolite repression","volume":"119","author":"Xiao","year":"2015","journal-title":"J. Biosci. Bioeng."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1385\/ABAB:115:1-3:1041","article-title":"Comparison of Two Posthydrolysis Processes of Brewery\u2019s Spent Grain Autohydrolysis Liquor to Produce a Pentose-Containing Culture Medium","volume":"115","author":"Duarte","year":"2004","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.biortech.2017.03.039","article-title":"Autohydrolysis pretreatment assessment in ethanol production from agave bagasse","volume":"242","author":"Martinez","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.jbiotec.2011.06.017","article-title":"Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: Overview and limits","volume":"156","author":"Tashiro","year":"2011","journal-title":"J. Biotechnol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/3\/605\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:47:45Z","timestamp":1760028465000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/3\/605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,5]]},"references-count":63,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["microorganisms13030605"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13030605","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,5]]}}}