{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T06:44:35Z","timestamp":1768718675712,"version":"3.49.0"},"reference-count":28,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T00:00:00Z","timestamp":1749859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese public agency that supports science, technology and innovation, FCT (Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia)","award":["UIDB\/50006\/2025"],"award-info":[{"award-number":["UIDB\/50006\/2025"]}]},{"name":"Portuguese public agency that supports science, technology and innovation, FCT (Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia)","award":["UIDB\/00102\/2020 DOI:10.54499\/UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020 DOI:10.54499\/UIDB\/00102\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The presence of fluoroquinolone (FQ) residues in food-producing animals has raised concerns regarding antimicrobial resistance. This study evaluated the occurrence of FQ residues in the liver and kidneys of piglets and their association with resistance patterns in Escherichia coli and Enterococcus species from piglets\u2019 intestinal microbiota. FQ residues were found in 44% of livers and 23% of kidneys. Among 340 E. coli isolates from feces, resistance to ciprofloxacin (CIP) (43.2%) and multidrug resistance (MDR) (82.7%) were prominent. The presence of FQ in kidneys significantly increased the odds of CIP-resistant E. coli (OR = 2.94, p = 0.0013) and MDR (OR = 2.70, p = 0.0047). Enterococci (n = 130) were evenly distributed among the species E. faecalis, E. faecium, and Enterococcus spp. and presented resistance to CIP (34.6%). FQ in kidneys were associated with higher odds of CIP-resistant enterococci (OR = 3.78, p = 0.015). Interaction models revealed species-dependent responses, with Enterococcus spp. showing high resistance in the presence of FQ in kidneys (OR = 18, p = 0.006), while E. faecium exhibited lower resistance compared to E. faecalis. These findings emphasize the role of FQ residues in promoting bacterial resistance and underscore the need for the stricter regulation and routine surveillance of antimicrobial use in livestock to curb the spread of bacterial resistance to clinical drugs, and mitigate public health risks\u2014One Health.<\/jats:p>","DOI":"10.3390\/microorganisms13061389","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T04:06:24Z","timestamp":1750046784000},"page":"1389","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fluoroquinolone Residues in Piglet Viscera and Their Impact on Intestinal Microbiota Resistance: A One Health Approach"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8902-0213","authenticated-orcid":false,"given":"Olga","family":"Cardoso","sequence":"first","affiliation":[{"name":"Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, Faculdade de Farm\u00e1cia, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-0088","authenticated-orcid":false,"given":"Maria Manuel","family":"Donato","sequence":"additional","affiliation":[{"name":"CIMAGO (Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9tica e Oncobiologia), Faculdade de Medicina, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9649-4823","authenticated-orcid":false,"given":"Sara Carolina","family":"Henriques","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6043-819X","authenticated-orcid":false,"given":"Fernando","family":"Ramos","sequence":"additional","affiliation":[{"name":"Universidade de Coimbra, Faculdade de Farm\u00e1cia, REQUIMTE\/LAQV, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1007\/s10393-019-01415-5","article-title":"Critical Importance of a One Health Approach to Antimicrobial Resistance","volume":"16","author":"White","year":"2019","journal-title":"Ecohealth"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yang, F., Han, B., Gu, Y., and Zhang, K. 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