{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:46:38Z","timestamp":1760060798908,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bio-based Industries Joint Undertaking","award":["101023567"],"award-info":[{"award-number":["101023567"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The sustainable reuse of agro-industrial effluents requires a detailed understanding of their microbial composition, especially in the context of integrated vineyard\u2013winery ecosystems. This study investigated the bacterial communities present in winery effluents generated during the early stages of red wine production, using samples collected at a winery in the Set\u00fabal Peninsula, Portugal. Metagenomic analysis targeting the 16S rRNA gene was used to characterise microbial diversity and identify taxa with potential relevance for biotechnology and environmental applications. The effluents exhibited a diverse microbiome, including Prevotella paludivivens, species from the Lactobacillus genus, and members of the Clostridiaceae family, the latter representing about 5% of the total community. Functional profiling of lactic acid bacteria revealed the predominance of Oenococcus and Lactobacillus genera, highlighting adaptive traits that may be beneficial under stress conditions. These results suggest that winery effluents, often considered waste, harbour microbial communities with functional potential that extends beyond fermentation, contributing to a broader grape\u2013wine microbial system. The findings emphasise the value of studying winemaking byproducts as reservoirs of microbial diversity and as resources for developing innovative and sustainable applications in biotechnology and environmental management within the wine industry.<\/jats:p>","DOI":"10.3390\/microorganisms13092192","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:08:58Z","timestamp":1758276538000},"page":"2192","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling Bacterial Diversity in Portuguese Red Wine Effluents Through a Metagenomic Approach"],"prefix":"10.3390","volume":"13","author":[{"given":"Ana Gabriela","family":"Gomes","sequence":"first","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Campus do IPS\u2014Estefanilha, 2910-761 Set\u00fabal, Portugal"},{"name":"Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Rua Am\u00e9rico da Silva Marinho, 2839-001 Lavradio, Portugal"}]},{"given":"Ana Cl\u00e1udia","family":"Sousa","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Campus do IPS\u2014Estefanilha, 2910-761 Set\u00fabal, Portugal"},{"name":"Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Rua Am\u00e9rico da Silva Marinho, 2839-001 Lavradio, Portugal"}]},{"given":"Jo\u00e3o S.","family":"Carreira","sequence":"additional","affiliation":[{"name":"Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Rua Am\u00e9rico da Silva Marinho, 2839-001 Lavradio, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8222-4440","authenticated-orcid":false,"given":"Alberto","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Embrapa\u2014Empresa Brasileira de Pesquisa Agropecu\u00e1ria, Parque Esta\u00e7\u00e3o Biol\u00f3gica (PqEB), s\/n\u00ba, Bras\u00edlia CEP 70770-901, DF, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9310-9456","authenticated-orcid":false,"given":"Marta C.","family":"Justino","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Campus do IPS\u2014Estefanilha, 2910-761 Set\u00fabal, Portugal"},{"name":"Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Rua Am\u00e9rico da Silva Marinho, 2839-001 Lavradio, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2083-7217","authenticated-orcid":false,"given":"Carla Amarelo","family":"Santos","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Campus do IPS\u2014Estefanilha, 2910-761 Set\u00fabal, Portugal"},{"name":"Escola Superior de Tecnologia do Barreiro, Instituto Polit\u00e9cnico de Set\u00fabal, Rua Am\u00e9rico da Silva Marinho, 2839-001 Lavradio, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e1500323","DOI":"10.1126\/sciadv.1500323","article-title":"Four billion people facing severe water scarcity","volume":"2","author":"Mekonnen","year":"2016","journal-title":"Sci. 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