{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:34:25Z","timestamp":1766122465156,"version":"3.48.0"},"reference-count":74,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"FCT","award":["UIDB\/04565\/2020"],"award-info":[{"award-number":["UIDB\/04565\/2020"]}]},{"name":"FCT","award":["SFRH\/BD\/125527\/2016"],"award-info":[{"award-number":["SFRH\/BD\/125527\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Extreme hypersaline environments harbour a unique biodiversity capable of surviving in such habitats, including halophilic and halotolerant bacteria. Microbial adaptations to these environments comprehend two main strategies: the \u201csalt-in\u201d that involves a high intracellular concentration of salts (e.g., potassium), and the \u201csalt-out\u201d that relies on the accumulation of small organic compounds (e.g., glycine betaine and trehalose). These evolutionary haloadaptations, combined with natural population competitiveness, often promotes the production of distinctive antimicrobial compounds, highlighting hypersaline environments as promising rich sources of novel natural products with biotechnological potential. Aiming at enlarging the knowledge on the microbiota of two Portuguese salterns (Aveiro and Olh\u00e3o), microbial isolation was performed using salt and saline sediment samples. A total of 39 microbial isolates were obtained in a saline medium, affiliated with Bacillota, Pseudomonadota, Actinomycetota, and Rhodothermaeota and the archaeal phylum Euryarchaeota. All isolates are generally common in saline habitats, with most (79%) exhibiting a halotolerant profile. Regarding the presence of biosynthetic related genes, 28% of the isolates lacked type I genes for polyketide synthases or non-ribosomal peptide synthetases, 36% contained at least one of these genes, and 36% possessed both. This study provides evidence of the biotechnological potential of the microbiota from two Portuguese salterns.<\/jats:p>","DOI":"10.3390\/microorganisms13122867","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T15:53:46Z","timestamp":1765986826000},"page":"2867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Culture-Dependent Bioprospecting of Halophilic Microorganisms from Portuguese Salterns"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9273-966X","authenticated-orcid":false,"given":"Eduarda","family":"Almeida","sequence":"first","affiliation":[{"name":"Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"given":"Adrianna","family":"Jackiewicz","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-0540","authenticated-orcid":false,"given":"Maria de F\u00e1tima","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-3970","authenticated-orcid":false,"given":"Olga Maria","family":"Lage","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR LA, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6971","DOI":"10.1128\/AEM.01868-10","article-title":"Halophiles 2010: Life in Saline Environments","volume":"76","author":"Ma","year":"2010","journal-title":"Appl. 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