{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T03:43:21Z","timestamp":1778039001254,"version":"3.51.4"},"reference-count":22,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T00:00:00Z","timestamp":1548288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of Pharmacy University of Coimbra","award":["n.a."],"award-info":[{"award-number":["n.a."]}]},{"name":"FEDER and FCT","award":["UID\/NEU\/04539\/2013"],"award-info":[{"award-number":["UID\/NEU\/04539\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Horizontal gene transfer events provide the basis for extensive dissemination of antimicrobial resistance traits between bacterial populations. Conjugation is considered to be the most frequent mechanism behind new resistance acquisitions in clinical pathogens but does not fully explain the resistance patterns seen in some bacterial genera. Gene transfer by natural transformation has been described for numerous clinical isolates, including some Acinetobacter species. The main aim of this study was to determine to what extent clinical, resistant Acinetobacter spp. isolates, express competence for natural transformation. Twenty-two clinical Acinetobacter spp. isolates collected over a 16-year time period, from five different geographical separated and\/or distinct Portuguese Hospitals were tested for natural transformability. Fourteen isolates, including 11 A. baumannii, 2 A. nosocomialis and 1 Acinetobacter sp., were identified as competent on semisolid media facilitating surface-motility. Competent Acinetobacter isolates were found in all the hospitals tested. Furthermore, osmolarity was shown to influence the uptake of exogenous DNA by competent A. baumannii A118. Our study demonstrates that natural competence is common among clinical isolates of Acinetobacter spp., and hence likely an important trait for resistance acquisition.<\/jats:p>","DOI":"10.3390\/microorganisms7020030","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T11:12:48Z","timestamp":1548328368000},"page":"30","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp."],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8879-5113","authenticated-orcid":false,"given":"Sara","family":"Domingues","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-458 Coimbra, Portugal"},{"name":"Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Natasha","family":"Ros\u00e1rio","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-458 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3885-0652","authenticated-orcid":false,"given":"\u00c2ngela","family":"C\u00e2ndido","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-458 Coimbra, Portugal"}]},{"given":"Daniela","family":"Neto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-458 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7217-2114","authenticated-orcid":false,"given":"Kaare M.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Health, Oslo Metropolitan University, N-0130 Oslo, Norway"}]},{"given":"Gabriela J.","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-458 Coimbra, Portugal"},{"name":"Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"fiy195","DOI":"10.1093\/femsec\/fiy195","article-title":"Environmental dimensions of antibiotic resistance: Assessment of basic science gaps","volume":"94","author":"Smalla","year":"2018","journal-title":"FEMS Microbiol. 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