{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:45:33Z","timestamp":1760240733125,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","award":["Grant No 2604"],"award-info":[{"award-number":["Grant No 2604"]}],"id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/BIO\/04469\/2019"],"award-info":[{"award-number":["UID\/BIO\/04469\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["BioTecNorte operation (NORTE-01-0145-FEDER-000004)"],"award-info":[{"award-number":["BioTecNorte operation (NORTE-01-0145-FEDER-000004)"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been identified as an etiologic agent of an ocular lesion. All these species can be cultured in vitro, but their morphological identification by macroscopic and microscopic traits is difficult and imprecise due to their similarity. In this study, 34 isolates of Neoscytalidium spp. from 32 onychomycosis and two dermatomycosis cases in Medellin (Colombia) were identified at the species level using sequencing of the ITS1+5.8S+ITS2 nuclear rDNA region and MALDI-TOF mass spectrometry (MS). Neoscytalidium dimidiatum strain MUM 17.21 was used to construct the reference spectrum in the in-house library to identify the clinical isolates by MALDI-TOF MS. Additionally, N. dimidiatum PPC-216 and PLAB-055 strains were used to validate the in-house constructed reference spectra. Although four groups were observed in the dendrogram obtained from the proteins of each isolate profile, MALDI-TOF MS and sequencing results are in accordance, since all isolates were identified as N. dimidiatum.<\/jats:p>","DOI":"10.3390\/microorganisms7090306","type":"journal-article","created":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T03:16:12Z","timestamp":1567394172000},"page":"306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)"],"prefix":"10.3390","volume":"7","author":[{"given":"Sindy V.","family":"Fl\u00f3rez-Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n Dermatol\u00f3gica, Instituto de Investigaciones M\u00e9dicas, Facultad de Medicina, Universidad de Antioquia, Avenida Juan del Corral, 050010 Medell\u00edn, Colombia"}]},{"given":"Juan C.","family":"G\u00f3mez-Vel\u00e1squez","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico PROLAB, Miembro del Grupo Synlab, Calle 19 A, 050021 Medell\u00edn, Colombia"}]},{"given":"Natalia","family":"Loaiza-D\u00edaz","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico PROLAB, Miembro del Grupo Synlab, Calle 19 A, 050021 Medell\u00edn, Colombia"}]},{"given":"C\u00e9lia","family":"Soares","sequence":"additional","affiliation":[{"name":"CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho (MUM), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Carla","family":"Santos","sequence":"additional","affiliation":[{"name":"CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho (MUM), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Nelson","family":"Lima","sequence":"additional","affiliation":[{"name":"CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho (MUM), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Ana C.","family":"Mesa-Arango","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Dermatol\u00f3gica, Instituto de Investigaciones M\u00e9dicas, Facultad de Medicina, Universidad de Antioquia, Avenida Juan del Corral, 050010 Medell\u00edn, Colombia"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3114\/sim0020","article-title":"Phylogenetic lineages in the Botryosphaeriales: A systematic and evolutionary framework","volume":"76","author":"Slippers","year":"2013","journal-title":"Stud. Mycol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.5941\/MYCO.2016.44.2.79","article-title":"Morphology and Phylogeny of Neoscytalidium orchidacearum sp. nov. (Botryosphaeriaceae)","volume":"44","author":"Huang","year":"2016","journal-title":"Mycobiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/myc.12849","article-title":"Identification and biofilm development by a new fungal keratitis aetiologic agent","volume":"62","author":"Raymundo","year":"2019","journal-title":"Mycoses"},{"key":"ref_4","first-page":"e453","article-title":"Superficial black onychomycosis due to Neoscytalidium dimidiatum","volume":"31","author":"Noriega","year":"2017","journal-title":"J. Eur. Acad. Derm. Venereol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/myc.12157","article-title":"Non-dermatophyte mould onychomycosis: A clinical and epidemiological study at a dermatology referral centre in Bogota, Colombia","volume":"57","author":"Alvarado","year":"2014","journal-title":"Mycoses"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1099\/jmm.0.065292-0","article-title":"First report of rhinosinusitis caused by Neoscytalidium dimidiatum in Iran","volume":"63","author":"Bakhshizadeh","year":"2014","journal-title":"J. Med. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1128\/JCM.00206-15","article-title":"Pulmonary fungal infection caused by Neoscytalidium dimidiatum","volume":"53","author":"Dionne","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Garinet, S., Tourret, J., Barete, S., Arzouk, N., Meyer, I., Frances, C., Datry, A., Mazier, D., Barrou, B., and Fekkar, A. (2015). Invasive cutaneous Neoscytalidium infections in renal transplant recipients: A series of five cases. Bmc. Infect. Dis., 15.","DOI":"10.1186\/s12879-015-1241-0"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1128\/JCM.01874-08","article-title":"Invasive Scytalidium dimidiatum infection in an immunocompetent adult","volume":"47","author":"Elinav","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s11046-018-0308-z","article-title":"Deep cutaneous Neoscytalidium dimidiatum infection: Successful outcome with amphotericin B therapy","volume":"184","author":"Yang","year":"2019","journal-title":"Mycopathologia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3114\/sim0021","article-title":"The Botryosphaeriaceae: Genera and species known from culture","volume":"76","author":"Phillips","year":"2013","journal-title":"Stud. Mycol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6241","DOI":"10.1073\/pnas.1117018109","article-title":"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi","volume":"109","author":"Schoch","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.cll.2013.03.001","article-title":"MALDI-TOF mass spectrometry for microorganism identification","volume":"33","author":"Dingle","year":"2013","journal-title":"Clin. Lab. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sanit\u00e1Lima, M., Coutinho de Lucas, R., Lima, N., Polizeli Mde, L.M., and Santos, C. (2019). Fungal community ecology using MALDI-TOF MS demands curated mass spectral databases. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00315"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ijfoodmicro.2008.11.023","article-title":"A polyphasic approach to the identification of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from Portuguese almonds","volume":"129","author":"Rodrigues","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref_16","unstructured":"Innis, M., Gelfand, D., Sninsky, J., and White, T. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, Academic Press."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"580","DOI":"10.3109\/13693786.2013.770605","article-title":"Identification of the Trichophyton mentagrophytes complex species using MALDI-TOF mass spectrometry","volume":"51","author":"Packeu","year":"2013","journal-title":"Med. Mycol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Cassagne, C., Ranque, S., Normand, A.C., Fourquet, P., Thiebault, S., Planard, C., Hendrickx, M., and Piarroux, R. (2011). Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028425"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Schoch, C.L., Robbertse, B., Robert, V., Vu, D., Cardinali, G., Irinyi, L., Meyer, W., Nilsson, R.H., Hughes, K., and Miller, A.N. (2014). Finding needles in haystacks: Linking scientific names, reference specimens and molecular data for Fungi. Database (Oxford), 2014.","DOI":"10.1093\/database\/bau061"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1080\/15572536.2006.11832803","article-title":"Fusicoccum arbuti sp. nov. causing cankers on pacific madrone in western North America with notes on Fusicoccum dimidiatum, the correct name for Scytalidium dimidiatum and Nattrassia mangiferae","volume":"97","author":"Farr","year":"2005","journal-title":"Mycologia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1111\/j.1365-4632.2012.05509.x","article-title":"Onychomycosis in Cameroon: A clinical and epidemiological study among dermatological patients","volume":"51","author":"Fabrizi","year":"2012","journal-title":"Int. J. Derm."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.eimc.2013.01.009","article-title":"The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identification and typing","volume":"32","author":"Pereira","year":"2014","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_25","first-page":"608","article-title":"Epidemiology of superficial fungal diseases in French Guiana: A three-year retrospective analysis","volume":"49","author":"Simonnet","year":"2011","journal-title":"Med. Mycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1111\/myc.12633","article-title":"Performance of mycology and histopathology tests for the diagnosis of toenail onychomycosis due to filamentous fungi: Dermatophyte and non-dermatophyte moulds","volume":"60","author":"Lavorato","year":"2017","journal-title":"Mycoses"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1439-0507.1998.tb00381.x","article-title":"Keratin degradation by Scytalidium species and Fusarium solani","volume":"41","author":"Oycka","year":"1998","journal-title":"Mycoses"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1111\/j.1365-2230.2009.03698.x","article-title":"In vitro activity of fluconazole, itraconazole, voriconazole and terbinafine against fungi causing onychomycosis","volume":"35","author":"Bueno","year":"2010","journal-title":"Clin. Exp. Derm."},{"key":"ref_29","first-page":"593","article-title":"Importancia creciente de los g\u00e9neros Fusarium y Scytalidium como agentes de onicomicosis","volume":"9","author":"Zuluaga","year":"2001","journal-title":"Rev. Asoc. Col. Dermat."},{"key":"ref_30","first-page":"251","article-title":"Comportamiento de los agentes etiol\u00f3gicos de las onicomicosis en un laboratorio de micolog\u00eda de referencia (Medell\u00edn 1994\u20132003)","volume":"33","author":"Zuluaga","year":"2005","journal-title":"Med. Cutan. Iber. Lat. Am."},{"key":"ref_31","first-page":"187","article-title":"Nucleotide structure of the Scytalidium hyalinum and Scytalidium dimidiatum 18S subunit ribosomal RNA gene: Evidence for the insertion of a group IE intron in the rDNA gene of S. dimidiatum","volume":"208","author":"Lacroix","year":"2001","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1128\/JCM.06634-11","article-title":"Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization-time of flight mass spectrometry","volume":"50","author":"Alshawa","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ab.2010.11.025","article-title":"Fungal pigments inhibit the matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungi","volume":"411","author":"Buskirk","year":"2011","journal-title":"Anal. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1128\/JCM.01025-14","article-title":"Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry can accurately differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii","volume":"52","author":"Chen","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1139\/gen-2016-0046","article-title":"Fungal DNA barcoding","volume":"59","author":"Xu","year":"2016","journal-title":"Genome"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/9\/306\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:15:52Z","timestamp":1760188552000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/9\/306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,1]]},"references-count":35,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["microorganisms7090306"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms7090306","relation":{},"ISSN":["2076-2607"],"issn-type":[{"type":"electronic","value":"2076-2607"}],"subject":[],"published":{"date-parts":[[2019,9,1]]}}}