{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T22:04:18Z","timestamp":1776636258619,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P","award":["SFRH\/BD\/116516\/2016"],"award-info":[{"award-number":["SFRH\/BD\/116516\/2016"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P.","award":["IF\/01302\/2015"],"award-info":[{"award-number":["IF\/01302\/2015"]}]},{"name":"Global Health and Tropical Medicine Center","award":["UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["UID\/Multi\/04413\/2013"]}]},{"name":"ERD funds","award":["project CeRaViP (16_019\/0000759)"],"award-info":[{"award-number":["project CeRaViP (16_019\/0000759)"]}]},{"name":"ERD funds","award":["UNCE 204072"],"award-info":[{"award-number":["UNCE 204072"]}]},{"name":"Bayer Animal Health, Germany","award":["N.A."],"award-info":[{"award-number":["N.A."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Phlebotomine sand fly-borne pathogens such as Leishmania spp. and phleboviruses are emerging threats to humans and animals worldwide. The aim of this work was to evaluate the exposure of cats from Portugal to Toscana virus (TOSV) and Sandfly Fever Sicilian virus (SFSV) and assess the associated risk factors. The possible association between exposure to Phlebotomus perniciosus saliva with TOSV and SFSV was also investigated. Out of 369 cats tested, 18 (4.9%, n = 365) were seropositive for TOSV, and eight (2.2%, n = 367) were seropositive for SFSV. Multivariate logistic regression analysis showed that cats presenting clinical signs that were compatible with leishmaniosis and antibodies to TOSV had a significantly higher risk of being SFSV seropositive. The presence of antibodies to sand fly-borne viruses in cats indicate that these animals are frequently exposed to sand flies and transmitted pathogens. Data suggest that cats can be used to qualitatively monitor human exposure to TOSV and SFSV in endemic areas. The clinical impact of SFSV in cats\u2019 health should be investigated. The identification of the sand fly species responsible for the circulation of TOSV and SFSV in nature and the evaluation of the vectorial competence of P. perniciosus to SFSV should also be addressed.<\/jats:p>","DOI":"10.3390\/microorganisms7090339","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T11:26:34Z","timestamp":1568201194000},"page":"339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Antibody Response to Toscana Virus and Sandfly Fever Sicilian Virus in Cats Naturally Exposed to Phlebotomine Sand Fly Bites in Portugal"],"prefix":"10.3390","volume":"7","author":[{"given":"Andr\u00e9","family":"Pereira","sequence":"first","affiliation":[{"name":"Global Health and Tropical Medicine (GHMT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), 1349-008 Lisboa, Portugal"},{"name":"Medical Parasitology Unit, IHMT-UNL, 1349-008 Lisboa, Portugal"}]},{"given":"Nazli","family":"Ayhan","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix Marseille Univ, IRD 190, INSERM 1207, IHU M\u00e9diterran\u00e9e Infection), 13385 Marseille, France"}]},{"given":"Jos\u00e9 Manuel","family":"Crist\u00f3v\u00e3o","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHMT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), 1349-008 Lisboa, Portugal"},{"name":"Medical Parasitology Unit, IHMT-UNL, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3976-1619","authenticated-orcid":false,"given":"Hugo","family":"Vilhena","sequence":"additional","affiliation":[{"name":"Center for Investigation Vasco da Gama (CIVG), Department of Veterinary Medicine, Vasco da Gama Universitary School, 3020-210 Coimbra, Portugal"},{"name":"University Veterinary Hospital of Coimbra, 3020-210 Coimbra, Portugal"},{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"given":"\u00c2ngela","family":"Martins","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio da Arr\u00e1bida, 2925-538 Azeit\u00e3o, Portugal"}]},{"given":"Patr\u00edcia","family":"Cachola","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio do Algarve, 8000-072 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-2277","authenticated-orcid":false,"given":"Joaquim","family":"Henriques","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio de Berna, 1050-039 Lisboa, Portugal"}]},{"given":"M\u00f3nica","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Veterin\u00e1ria Porto Seguro, 8700-507 Olh\u00e3o, Portugal"}]},{"given":"Ana","family":"Catarino","sequence":"additional","affiliation":[{"name":"VetCoa - Servi\u00e7os Veterin\u00e1rios, 6320-354 Sabugal, Portugal"}]},{"given":"Tereza","family":"Lestinova","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic"}]},{"given":"Tatiana","family":"Spitzova","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic"}]},{"given":"Petr","family":"Volf","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic"}]},{"given":"Lenea","family":"Campino","sequence":"additional","affiliation":[{"name":"Medical Parasitology Unit, IHMT-UNL, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7675-8251","authenticated-orcid":false,"given":"Remi","family":"Charrel","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix Marseille Univ, IRD 190, INSERM 1207, IHU M\u00e9diterran\u00e9e Infection), 13385 Marseille, France"}]},{"given":"Carla","family":"Maia","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHMT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), 1349-008 Lisboa, Portugal"},{"name":"Medical Parasitology Unit, IHMT-UNL, 1349-008 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1365-2915.2012.01034.x","article-title":"Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern","volume":"27","author":"Maroli","year":"2013","journal-title":"Med. 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