{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:36:40Z","timestamp":1763037400579,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T00:00:00Z","timestamp":1576886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Sporotrichosis is a globally distributed subcutaneous fungal infection caused by dimorphic fungi belonging to the Sporothrix species complex that affects the skin of limbs predominantly, but not exclusively. A rare case of ocular sporotrichosis in an immunocompetent Brazilian patient from the countryside of Rio de Janeiro State is reported. A 68-year-old woman presented with a subconjunctival infiltrative lesion in the right eye with pre-auricular lymphadenopathy of onset 4 months ago that evolved to suppurative nodular lesions on the eyelids. Conjunctival secretion was evaluated by histopathological examination and inoculated on Sabouraud Dextrose Agar (SDA). Histopathology showed oval bodies within giant cells and other mononucleated histiocytes. Fungus grown on SDA was identified as Sporothrix sp. by morphological observations. The isolated strain was finally identified by Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) associated with an in-house database enriched with reference Sporothrix complex spectra. The strain presented a MALDI spectrum with the ion peaks of the molecular mass profile of S. brasiliensis. The patient was adequately treated with amphotericin B subsequently replaced by itraconazole. Due to scars left by the suppurative process, the patient presented poor final visual acuity. The present work presents an overview of ocular sporotrichosis and discusses the diagnostic difficulty that can lead to visual sequelae in these cases.<\/jats:p>","DOI":"10.3390\/microorganisms8010022","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T03:15:01Z","timestamp":1577070901000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient"],"prefix":"10.3390","volume":"8","author":[{"given":"Aline","family":"M. F. Matos","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, University Hospital of the Federal University of Juiz de Fora, Juiz de Fora 36038-330, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8084-7513","authenticated-orcid":false,"given":"Lucas","family":"M. Moreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}]},{"given":"Bianca","family":"F. Barczewski","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of the Federal University of Juiz de Fora, Juiz de Fora 36038-330, Brazil"}]},{"given":"Lucas","family":"X. de Matos","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of the Federal University of Juiz de Fora, Juiz de Fora 36038-330, Brazil"}]},{"given":"Jordane","family":"B. V. de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of the Federal University of Juiz de Fora, Juiz de Fora 36038-330, Brazil"}]},{"given":"Maria Ines","family":"F. Pimentel","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6639-6058","authenticated-orcid":false,"given":"Rodrigo","family":"Almeida-Paes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}]},{"given":"Murilo","family":"G. Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil"}]},{"given":"Tatiana","family":"C. A. Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2185-0613","authenticated-orcid":false,"given":"Nelson","family":"Lima","sequence":"additional","affiliation":[{"name":"CEB\u2014Biological Engineering Centre, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Magnum","family":"de O. Matos","sequence":"additional","affiliation":[{"name":"Imaging Department of Instituto Oncol\u00f3gico, Hospital Nove de Julho, Juiz de Fora 36010-510, Brazil"}]},{"given":"Louise G.","family":"de M. e Costa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine of Federal University of Juiz de Fora, Juiz de Fora 36038-330, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-0941","authenticated-orcid":false,"given":"Cledir","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Natural Resources, BIOREN-UFRO, Universidad de La Frontera, 4811-230 Temuco, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7670-9225","authenticated-orcid":false,"given":"Manoel","family":"Marques Evangelista Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica em Dermatozoonoses em Animais Dom\u00e9sticos, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"},{"name":"Laborat\u00f3rio de Taxonomia, Bioqu\u00edmica e Bioprospec\u00e7\u00e3o de Fungos, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3198","DOI":"10.1128\/JCM.00808-07","article-title":"Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest","volume":"45","author":"Marimon","year":"2007","journal-title":"J. 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