{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:17:29Z","timestamp":1771035449172,"version":"3.50.1"},"reference-count":128,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Entomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review the current techniques that have been developed so far, in the last five decades, and display brief protocols which can be adopted for the isolations of these entomopathogens. Among bacteria, this review focuses on Serratia spp. and bacteria from the class Bacilli. Among fungi, the review focuses those from the order Hypocreales, for example, genera Beauveria, Clonostachys, Lecanicillium, Metarhizium, and Purpureocillium. The authors chose these groups of entomopathogenic bacteria and fungi based on their importance in the microbial biopesticide market.<\/jats:p>","DOI":"10.3390\/microorganisms9010016","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T08:38:43Z","timestamp":1608712723000},"page":"16","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi"],"prefix":"10.3390","volume":"9","author":[{"given":"Lav","family":"Sharma","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Tr\u00e1s-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8433-5892","authenticated-orcid":false,"given":"Nitin","family":"Bohra","sequence":"additional","affiliation":[{"name":"Max Planck School Matter to Life, Max Planck Institute for Medical Research, Jahnstra\u00dfe 29, 69120 Heidelberg, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6802-4805","authenticated-orcid":false,"given":"Vishnu D.","family":"Rajput","sequence":"additional","affiliation":[{"name":"Soil Science and Land Evaluation Department, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1950-4810","authenticated-orcid":false,"given":"Francisco Roberto","family":"Quiroz-Figueroa","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, Centro Interdisciplinario de Investigaci\u00f3n para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-IPN Unidad Sinaloa), Laboratorio de Fitomejoramiento Molecular, Blvd. Juan de Dios B\u00e1tiz Paredes no. 250, Col. San Joach\u00edn, C.P., Guasave 81101, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2536-1967","authenticated-orcid":false,"given":"Rupesh Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica de Vila Real, Universidade de Tr\u00e1s-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0963-5785","authenticated-orcid":false,"given":"Guilhermina","family":"Marques","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Tr\u00e1s-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ruiu, L. (2018). Microbial Biopesticides in Agroecosystems. 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