{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T07:12:02Z","timestamp":1768720322909,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["IZS AM 02\/19 RC"],"award-info":[{"award-number":["IZS AM 02\/19 RC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>With most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of Campylobacter, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of Campylobacter spp. in different canine populations in Portugal, and to evaluate its zoonotic potential through genomic analysis. From a total of 125 rectal swabs collected from companion (n = 71) and hunting dogs (n = 54) living in two different settings, rural (n = 75) and urban (n = 50), 32 Campylobacter spp. isolates were obtained. Four different Campylobacter species were identified by Multiplex PCR and MALDI-TOF mass spectrometry, of which Campylobacter jejuni (n = 14, 44%) was overall the most frequently found species. Relevant resistance phenotypes were detected in C. jejuni, with 93% of the isolates being resistant to ciprofloxacin, 64% to tetracycline, and 57% to ampicillin, and three isolates being multi-drug-resistant. Comparison of the phenotypic and genotypic traits with human isolates from Portuguese patients revealed great similarity between both groups. Particularly relevant, the wgMLST analysis allowed the identification of isolates from human and dogs without any apparent epidemiological relationship, sharing high genetic proximity. Notwithstanding the limited sample size, considering the high genomic diversity of C. jejuni, the genetic overlap between human and dog strains observed in this study confirmed that the occurrence of this species in dogs is of public health concern, reinforcing the call for a One Health approach.<\/jats:p>","DOI":"10.3390\/microorganisms9112231","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T23:54:33Z","timestamp":1635292473000},"page":"2231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Campylobacter jejuni in Different Canine Populations: Characteristics and Zoonotic Potential"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-0393","authenticated-orcid":false,"given":"Maria-Leonor","family":"Lemos","sequence":"first","affiliation":[{"name":"ICBAS\u2014Institute of Biomedical Sciences Abel Salazar, University of Porto, 4050313 Porto, Portugal"}]},{"given":"Alexandra","family":"Nunes","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department, National Institute of Health Dr. Ricardo Jorge, 1649016 Lisbon, Portugal"},{"name":"Faculty of Veterinary Medicine, Lus\u00f3fona University, 1300477 Lisboa, Portugal"},{"name":"CBIOS\u2014Universidade Lus\u00f3fona\u2019s Research Center for Biosciences & Health Technologies, Campo Grande 376, 1749024 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7121-6373","authenticated-orcid":false,"given":"Massimo","family":"Ancora","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-1195","authenticated-orcid":false,"given":"Cesare","family":"Camm\u00e0","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6115-8811","authenticated-orcid":false,"given":"Paulo Martins da","family":"Costa","sequence":"additional","affiliation":[{"name":"ICBAS\u2014Institute of Biomedical Sciences Abel Salazar, University of Porto, 4050313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6360-2576","authenticated-orcid":false,"given":"M\u00f3nica","family":"Oleastro","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department, National Institute of Health Dr. Ricardo Jorge, 1649016 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control) (2021). The European Union One Health 2019 Zoonoses Report. 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