{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:49:20Z","timestamp":1778726960398,"version":"3.51.4"},"reference-count":23,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17\/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17\/2.8 cells indicating that ROS 17\/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.<\/jats:p>","DOI":"10.3390\/molecules13112729","type":"journal-article","created":{"date-parts":[[2008,10,31]],"date-time":"2008-10-31T08:38:56Z","timestamp":1225442336000},"page":"2729-2739","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin"],"prefix":"10.3390","volume":"13","author":[{"given":"Tim","family":"Scharnweber","sequence":"first","affiliation":[{"name":"Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein- Leopoldshafen Karlsruhe, Germany"}]},{"given":"Catarina","family":"Santos","sequence":"additional","affiliation":[{"name":"Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein- Leopoldshafen Karlsruhe, Germany"},{"name":"Department of Ceramic and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Ralf-Peter","family":"Franke","sequence":"additional","affiliation":[{"name":"Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein- Leopoldshafen Karlsruhe, Germany"},{"name":"Department of Biomaterials, University of Ulm, 89081 Ulm, Germany"}]},{"given":"Maria Margarida","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Ceramic and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Maria Elisabete V.","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Ceramic and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2008,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.2174\/092986707782023208","article-title":"Modification of apatite materials for bone tissue engineering and drug delivery carriers","volume":"14","author":"Matsumoto","year":"2007","journal-title":"Curr. 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