{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T09:37:54Z","timestamp":1768988274439,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2010,12,30]],"date-time":"2010-12-30T00:00:00Z","timestamp":1293667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Croton nepetaefolius is a native plant from northeastern Brazil that belongs to the Euphorbiaceae family. The biological action of this plant has been extensively explored, being the secondary metabolites responsible for its properties alkaloids, diterpenes, and triterpenes. This study aimed to evaluate the ability of casbane diterpene (CD), isolated from the ethanolic extract of C. nepetaefolius, to inhibit microbial growth and biofilm formation of several clinical relevant species (bacteria and yeasts). It was found that CD possessed biocidal and biostatic activity against the majority of the species screened, with minimal active concentrations ranging between 125 and 500 \u00b5g\/mL. In addition, it was observed that biofilm formation was inhibited even when the planktonic growth was not significantly affected. In conclusion, CD showed potential to be a natural tool for the treatment of diseases caused by different infectious microorganisms.<\/jats:p>","DOI":"10.3390\/molecules16010190","type":"journal-article","created":{"date-parts":[[2010,12,30]],"date-time":"2010-12-30T10:33:15Z","timestamp":1293705195000},"page":"190-201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Casbane Diterpene as a Promising Natural Antimicrobial Agent against Biofilm-Associated Infections"],"prefix":"10.3390","volume":"16","author":[{"given":"Victor Alves","family":"Carneiro","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine of Sobral, Federal University of Cear\u00e1, Fortaleza, CE, Brazil"}]},{"given":"H\u00e9lcio Silva dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Centre of the Exact Sciences and Technology, Acara\u00fa Valley State University, 62040-370, Sobral, CE, Brazil"}]},{"given":"Francisco Vassiliepe Sousa","family":"Arruda","sequence":"additional","affiliation":[{"name":"Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, 60740-000, Fortaleza, CE, Brazil"}]},{"given":"Paulo Nogueira","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Centre of the Exact Sciences and Technology, Acara\u00fa Valley State University, 62040-370, Sobral, CE, Brazil"}]},{"given":"Maria Rose Jane Ribeiro","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Centre of the Exact Sciences and Technology, Acara\u00fa Valley State University, 62040-370, Sobral, CE, Brazil"}]},{"given":"Maria Ol\u00edvia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Centre for Biological Engineering, IBB-Institute for Biotechnology and Bioengineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0317-4877","authenticated-orcid":false,"given":"Mariana","family":"Henriques","sequence":"additional","affiliation":[{"name":"Centre for Biological Engineering, IBB-Institute for Biotechnology and Bioengineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Benildo Sousa","family":"Cavada","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine of Sobral, Federal University of Cear\u00e1, Fortaleza, CE, Brazil"}]},{"given":"Edson Holanda","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine of Sobral, Federal University of Cear\u00e1, Fortaleza, CE, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2010,12,30]]},"reference":[{"key":"ref_1","first-page":"578","article-title":"Bacterial polymers: biosynthesis, modifications and applications","volume":"8","author":"Rehm","year":"2010","journal-title":"Appl. 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