{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:27:46Z","timestamp":1760866066279,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Diacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to evaluate the microalgae-biomass assimilation and its health-benefits, single-dose (CD1-mice) studies were followed by 66-days repeated-dose study in Wistar rats with the highest tested single-dose of microalgae equivalent to 101 mg\/kg eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA). Microalgae-supplementation modulated EPA and docosapentaenoic acid enrichment at arachidonic acid content expenditure in erythrocytes and liver, while increasing EPA content of heart and adipose tissues of rats. Those fatty acid (FA) changes confirmed the D. vlkianum-biomass FA assimilation. The principal component analyses discriminated brain from other tissues, which formed two other groups (erythrocytes, liver, and heart separated from kidney and adipose tissues), pointing to a distinct signature of FA deposition for the brain and for the other organs. The improved serum lipid profile, omega-3 index and erythrocyte plasticity support the cardiovascular benefits of D. vlkianum. These results bolster the potential of D. vlkianum-biomass to become a \u201cheart-healthy\u201d food supplement providing a safe and renewable source of bioavailable omega-3 FA.<\/jats:p>","DOI":"10.3390\/molecules22071097","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T10:27:31Z","timestamp":1499077651000},"page":"1097","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2679-2379","authenticated-orcid":false,"given":"Cristina","family":"De Mello-Sampayo","sequence":"first","affiliation":[{"name":"Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisbon 1649-003, Portugal"}]},{"given":"Angela","family":"Paterna","sequence":"additional","affiliation":[{"name":"Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisbon 1649-003, Portugal"}]},{"given":"Ambra","family":"Polizzi","sequence":"additional","affiliation":[{"name":"Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisbon 1649-003, Portugal"}]},{"given":"Diana","family":"Duarte","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalh\u00e3es Ramalho, Lisbon 1495-006, Portugal"}]},{"given":"Irineu","family":"Batista","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalh\u00e3es Ramalho, Lisbon 1495-006, Portugal"}]},{"given":"Rui","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisbon 1649-003, Portugal"}]},{"given":"Patr\u00edcia","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Division of Modelling and Marine Resources, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalh\u00e3es Ramalho, Lisbon 1495-006, Portugal"}]},{"given":"Anabela","family":"Raymundo","sequence":"additional","affiliation":[{"name":"LEAF (Linking Landscape Environment Agriculture and Food), Research Center. Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"given":"Ana","family":"Batista","sequence":"additional","affiliation":[{"name":"LEAF (Linking Landscape Environment Agriculture and Food), Research Center. Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"given":"Lu\u00edsa","family":"Gouveia","sequence":"additional","affiliation":[{"name":"LNEG_Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"given":"Beatriz","family":"Silva-Lima","sequence":"additional","affiliation":[{"name":"Department of Pharmacological Sciences, iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisbon 1649-003, Portugal"}]},{"given":"Narcisa","family":"Bandarra","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Upgrading, Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Rua Alfredo Magalh\u00e3es Ramalho, Lisbon 1495-006, Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, P 4050-123 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2017,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1007\/BF02537207","article-title":"Digestion and Absorption of Free and Esterified Fish Oil Fatty Acids in Rats","volume":"26","author":"Schrijver","year":"1991","journal-title":"Lipids"},{"key":"ref_2","first-page":"21","article-title":"Microalgae-source of natural bioactives as functional ingredients","volume":"7","author":"Gouveia","year":"2010","journal-title":"Food Sci. Technol. Bull."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1745-4522.1996.tb00073.x","article-title":"Microalgae as a source of fatty acids","volume":"3","author":"Behrens","year":"1996","journal-title":"J. Food Lipid"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/CS20040119","article-title":"n-3 Fatty acids and cardiovascular disease: Evidence explained and mechanisms explored","volume":"107","author":"Calder","year":"2004","journal-title":"Clin. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.lwt.2012.05.006","article-title":"Isochrysis galbana and Diacronema vlkianum biomass incorporation in pasta products as PUFA\u2019s source","volume":"50","author":"Fradique","year":"2013","journal-title":"LWT Food Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.plipres.2006.01.001","article-title":"Lipids and lipid metabolism in eukaryotic algae","volume":"45","author":"Guschina","year":"2006","journal-title":"Prog. Lipid Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.explore.2009.07.001","article-title":"From fish oil to microalgae oil: A win-win shift for humans and our habitat","volume":"5","author":"Cannon","year":"2009","journal-title":"Explore"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.fct.2008.11.014","article-title":"An n-3 PUFA-rich microalga oil diet protects to a similar extent as a fish oil-rich diet against AOM-induced colonic aberrant crypt foci in F344 rats","volume":"47","author":"Beelen","year":"2009","journal-title":"Food Chem. Toxicol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.algal.2013.01.004","article-title":"Comparison of microalgal biomass profiles as novel functional ingredient for food products","volume":"2","author":"Batista","year":"2013","journal-title":"Algal Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10499-008-9211-9","article-title":"Effect of temperature on a-tocopherol, fatty acid profile, and pigments of Diacronema vlkianum (Haptophyceae)","volume":"17","author":"Durmaz","year":"2009","journal-title":"Aquacult. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1745-4522.2003.tb00020.x","article-title":"Fatty acids, sterols, \u03b1-tocopherol and total carotenoids composition of Diacronema vlkianum","volume":"10","author":"Donato","year":"2003","journal-title":"J. Food Lipid"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1111\/j.0022-3646.1997.01016.x","article-title":"Unusual dihydroxysterols as chemotaxonomic markers for microalgae from the order Pavlovales (Haptophyceae)","volume":"33","author":"Volkman","year":"1997","journal-title":"J. Phycol."},{"key":"ref_13","unstructured":"U.S. Department of Health and Human Services, Food and Drug Administration, and Center for Drug Evaluation and Research (CDER) (2005). Guidance for Industry: Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1161\/CIRCULATIONAHA.106.179294","article-title":"Primary Prevention of Cardiovascular Diseases in People With Diabetes Mellitus A Scientific Statement from the American Heart Association and the American Diabetes Association","volume":"115","author":"Buse","year":"2007","journal-title":"Circulation"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.atherosclerosis.2007.08.024","article-title":"European guidelines on cardiovascular disease prevention in clinical practice: Executive summary","volume":"194","author":"Graham","year":"2007","journal-title":"Atherosclerosis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1161\/CIRCULATIONAHA.106.174516","article-title":"AHA\/ACC guidelines for secondary prevention for patients with coronary and other atherosclerotic vascular disease: 2006 update: Endorsed by the National Heart, Lung, and Blood Institute","volume":"113","author":"Smith","year":"2006","journal-title":"Circulation"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.plefa.2013.01.001","article-title":"Distinct regulation of plasma LDL cholesterol by eicosapentaenoic and docosahexaenoic acid in high fat diet- fed hamsters: Participation of cholesterol ester transfer protein and LDL receptor","volume":"88","author":"Ishida","year":"2013","journal-title":"Prostaglandins Leukot Essent Fatty Acids"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.jdermsci.2015.06.015","article-title":"Oral supplementation with fish oil reduces dryness and pruritus in the acetone-induced dry skin rat model","volume":"79","author":"Barcelos","year":"2015","journal-title":"J. Dermatol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.nutres.2015.12.015","article-title":"Docosahexaenoic acid at the sn-2 position of structured triacylglycerols improved n-3 polyunsaturated fatty acid assimilation in tissues of hamsters","volume":"36","author":"Bandarra","year":"2016","journal-title":"Nutr. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1745-4514.1999.tb00014.x","article-title":"Effects of structured lipid containing omega 3 and medium chain fatty acids on serum lipids and immunological variables in mice","volume":"23","author":"Lee","year":"1999","journal-title":"J. Food Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1161\/01.CIR.102.11.1264","article-title":"Differential effects of eicosapentaenoic acid and docosahexaenoic acid on vascular reactivity of the forearm microcirculation in hyperlipidemic, overweight men","volume":"102","author":"Mori","year":"2000","journal-title":"Circulation"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1146\/annurev-nutr-071812-161230","article-title":"Omega-3 Fatty Acids, Hepatic Lipid Metabolism, and Nonalcoholic Fatty Liver Disease","volume":"33","author":"Scorletti","year":"2013","journal-title":"Annu. Rev. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0005-2760(93)90049-F","article-title":"Changes of n-3 and n-6 fatty acids in plasma and circulating cells of normal subjects, after prolonged administration of 20:5 (EPA) and 22:6 (DHA) ethyl esters and prolonged washout","volume":"1210","author":"Marangoni","year":"1993","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.algal.2013.07.005","article-title":"Insights on the safety of carotenogenic Chlorella vulgaris in rodents","volume":"2","author":"Corvo","year":"2013","journal-title":"Algal Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1124\/dmd.107.015248","article-title":"Interspecies comparisons of pharmacokinetics and pharmacodynamics of recombinant human erythropoietin","volume":"35","author":"Woo","year":"2007","journal-title":"Drug Metab. Dispos."},{"key":"ref_26","first-page":"20","article-title":"Red blood cell membrane fatty acids as a diagnostic test","volume":"12","author":"Jackson","year":"1997","journal-title":"J. Orthomol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1161\/01.CIR.0000142292.10048.B2","article-title":"Omega-3 Fatty Acids in Cardiac Biopsies From Heart Transplantation Patients - Correlation With Erythrocytes and Response to Supplementation","volume":"110","author":"Harris","year":"2004","journal-title":"Circulation"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1467S","DOI":"10.1093\/ajcn\/83.6.1467S","article-title":"Distribution, interconversion, and dose response of n-3 fatty acids in humans","volume":"83","author":"Arterburn","year":"2006","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2981","DOI":"10.1016\/j.bbagen.2013.01.018","article-title":"Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-kB pathway in macrophages and splenocytes","volume":"1830","author":"Ku","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.livsci.2006.01.009","article-title":"Effects of a dietary supplement of DHA-rich marine algae on Italian heavy pig production parameters","volume":"103","author":"Sardi","year":"2006","journal-title":"Livest. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1159\/000177797","article-title":"Dietary lipids from marine unicellular algae enhance the amount of liver and blood omega-3 fatty acids in rats","volume":"38","author":"Sukenik","year":"1994","journal-title":"Ann. Nutr. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.arcmed.2005.08.005","article-title":"Protective effect of Arthrospira maxima on fatty acid composition in fatty liver","volume":"37","author":"Mascher","year":"2006","journal-title":"Arch. Med. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1093\/ajcn\/76.4.750","article-title":"Adipose tissue biomarkers of fatty acid intake","volume":"76","author":"Baylin","year":"2002","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3164\/jcbn.14.151","article-title":"Comparative effects of eicosapentaenoic acid and docosahexaenoic acid on n-6 and n-3 fatty acid profiles of phospholipid classes in several tissues of rats fed a hypertriglyceridemic diet","volume":"14","author":"Taniguchi","year":"1993","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1021\/jf970385d","article-title":"Dose-response effect of dietary docosahexaenoic acid on fatty acid profiles of serum and tissue lipids in rats","volume":"46","author":"Saito","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/S0022-2275(20)32132-5","article-title":"Developmental changes in rat brain membrane lipids and fatty acids\u2014The preferential prenatal accumulation of docosahexaenoic acid","volume":"40","author":"Green","year":"1999","journal-title":"J. Lipid Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1093\/clinchem\/18.6.499","article-title":"Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge","volume":"18","author":"Friedewald","year":"1972","journal-title":"Clin. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.scitotenv.2006.03.006","article-title":"Evaluation of total lipids using enzymatic methods for the normalization of persistent organic pollutant levels in serum","volume":"366","author":"Covaci","year":"2006","journal-title":"Sci. Total Environ."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-2621.1997.tb04364.x","article-title":"Seasonal changes in lipid composition of sardine Sardinapilchardus","volume":"62","author":"Bandarra","year":"1997","journal-title":"J. Food Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"749","DOI":"10.3168\/jds.2007-0265","article-title":"Principal component and multivariate analysis of milk long-chain fatty acid composition during diet-induced milk fat depression","volume":"91","author":"Kadegowda","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_41","unstructured":"R Core Team (2017, February 01). R: A language and environment for statistical computing. Available online: http:\/\/www.R-project.org\/."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2009). ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-0-387-98141-3"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1097\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:41:06Z","timestamp":1760208066000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,1]]},"references-count":42,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7]]}},"alternative-id":["molecules22071097"],"URL":"https:\/\/doi.org\/10.3390\/molecules22071097","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2017,7,1]]}}}