{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T21:16:48Z","timestamp":1772227008285,"version":"3.50.1"},"reference-count":26,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T00:00:00Z","timestamp":1556150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RTC-2016-5836-2"],"award-info":[{"award-number":["RTC-2016-5836-2"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL2016-75332-C2-1-R"],"award-info":[{"award-number":["AGL2016-75332-C2-1-R"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A new method using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS\/MS) methodology was developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) contents in liquid and solid vegetable samples. The advantages of this method are speed, high sensitivity and practical application. In accordance with these advantages, the present method allows the simultaneous determination of AA and DHAA without previous reduction\/derivatization of DHAA and without the use of internal standards in the samples. This is of high interest in routine analysis, providing a simpler sample preparation, as well as enhanced accuracy and robustness. Its validation included selectivity, sensitivity and linearity, precision and accuracy, matrix effect, and recovery. The results showed high selectivity and sensitivity, with calibration curves ranging from 10 to 500 ng mL\u22121 and from 50 to 500 ng mL\u22121 for AA and DHAA, respectively. Appropriate dilutions for each sample are necessary to avoid the matrix effect with accepted recoveries.<\/jats:p>","DOI":"10.3390\/molecules24081632","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T10:44:06Z","timestamp":1556189046000},"page":"1632","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["New UHPLC-QqQ-MS\/MS Method for the Rapid and Sensitive Analysis of Ascorbic and Dehydroascorbic Acids in Plant Foods"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7404-2623","authenticated-orcid":false,"given":"Nieves","family":"Baenas","sequence":"first","affiliation":[{"name":"Institute of Nutritional Medicine, University Medical Center Schleswig-Holstein, Campus L\u00fcbeck, Ratzeburger Allee 160, 23538 L\u00fcbeck, Germany"}]},{"given":"Francisco J.","family":"Salar","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Food Lab, Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Sciences and Technology, CEBAS-CSIC, University Campus of Espinardo, Edif. 25, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-712X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Dom\u00ednguez-Perles","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Food Lab, Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Sciences and Technology, CEBAS-CSIC, University Campus of Espinardo, Edif. 25, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-3917","authenticated-orcid":false,"given":"Cristina","family":"Garc\u00eda-Viguera","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Food Lab, Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Sciences and Technology, CEBAS-CSIC, University Campus of Espinardo, Edif. 25, 30100 Murcia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dasgupta, A., and Klein, K. (2014). Chapter 15-Antioxidant Vitamins and Minerals, in Antioxidants. Food, Vitamins and Supplements, Elsevier.","DOI":"10.1016\/B978-0-12-405872-9.00015-X"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Johnston, C.S. (2012). Vitamin C. Present Knowledge in Nutrition, Wiley-Blackwell. [10th ed.].","DOI":"10.1002\/9781119946045.ch16"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.jpba.2009.07.027","article-title":"Natural bioactive compounds of Citrus limon for food and health","volume":"51","author":"Moreno","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.2741\/4160","article-title":"Effects of vitamin C on health: a review of evidence","volume":"18","author":"Grosso","year":"2013","journal-title":"Front Biosci. (Landmark Ed)"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.4081\/ijfs.2016.4313","article-title":"The Use of Ascorbic Acid as a Food Additive: Technical-Legal Issues","volume":"5","author":"Varvara","year":"2016","journal-title":"IJFS"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF00762775","article-title":"The role of ascorbate in antioxidant protection of biomembranes: Interaction with vitamin E and coenzyme Q","volume":"26","author":"Beyer","year":"1994","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1096\/fj.05-4107com","article-title":"Vitamin C enters mitochondria via facilitative glucose transporter 1 (Glut1) and confers mitochondrial protection against oxidative injury","volume":"19","author":"Sagun","year":"2005","journal-title":"FASEB J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1021\/jf010910f","article-title":"Very Low Vitamin C Activity of Orally Administered l-Dehydroascorbic Acid","volume":"50","author":"Ogiri","year":"2002","journal-title":"J. Agric. Food. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.12923\/j.2084-980X\/26.4\/a.21","article-title":"A comparison of vitamin C content determination by chromatographic and spectrophotometric methods according to standard PN-A-040","volume":"26","author":"Mazurek","year":"2013","journal-title":"Current Issues Pharm. Med. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.foodchem.2014.10.065","article-title":"Precision of dehydroascorbic acid quantitation with the use of the subtraction method--validation of HPLC-DAD method for determination of total vitamin C in food","volume":"173","author":"Mazurek","year":"2015","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/j.trac.2008.08.006","article-title":"HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids","volume":"27","author":"Solich","year":"2008","journal-title":"Trends Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1007\/s12161-011-9347-5","article-title":"Optimization and Validation of a UV\u2013HPLC Method for Vitamin C Determination in Strawberries (Fragaria ananassa Duch.) Using Experimental Designs","volume":"5","author":"Pirovani","year":"2012","journal-title":"Food Anal. Methods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0378-4347(99)00193-0","article-title":"Improved method for simultaneous determination of ascorbic acid and dehydroascorbic acid, isoascorbic acid and dehydroisoascorbic acid in food and biological samples","volume":"730","author":"Kall","year":"1999","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0928-0987(02)00258-0","article-title":"Biochemical changes in arthritic rats: Dehydroascorbic and ascorbic acid levels","volume":"18","author":"Cruz","year":"2003","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.foodchem.2010.12.140","article-title":"Simultaneous determination of ascorbic and dehydroascorbic acids in vegetables and fruits by liquid chromatography with tandem-mass spectrometry","volume":"127","author":"Fenoll","year":"2011","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.foodchem.2017.09.002","article-title":"Improved analysis of anthocyanins and vitamin C in blue-purple potato cultivars","volume":"242","author":"Vuorinen","year":"2018","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1007\/s00216-011-5668-x","article-title":"An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage","volume":"403","author":"Spinola","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.foodchem.2014.11.104","article-title":"Comparison of UPLC and HPLC methods for determination of vitamin C","volume":"175","author":"Klimczak","year":"2015","journal-title":"Food Chem."},{"key":"ref_19","unstructured":"(2019, January 10). Harmonised Tripartite Guideline. Guidelines on Validation of Analytical Procedures, Text and Methodology Q2 (R1). International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human use (ICH). November 2005. Available online: https:\/\/www.ich.org\/fileadmin\/Public_Web_Site\/ICH_Products\/Guidelines\/Quality\/Q2_R1\/Step4\/Q2_R1__Guideline.pdf."},{"key":"ref_20","unstructured":"(2019, January 10). Bioanalytical Method Validation. Guidance for Industry. U.S. Department of Health and Human Services, Food and Drug Administration (FDA), Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM), Biopharmaceutics. May 2018, Available online: https:\/\/www.fda.gov\/downloads\/drugs\/guidances\/ucm070107.pdf."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.chroma.2012.08.029","article-title":"Matrix effects and recovery calculations in analyses of pharmaceuticals based on the determination of \u03b2-blockers and \u03b2-agonists in environmental samples","volume":"1258","author":"Caban","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.jfca.2009.01.019","article-title":"Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli","volume":"22","author":"Koh","year":"2009","journal-title":"J. Food Composit. Anal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.postharvbio.2005.02.016","article-title":"Effects of heat treatment on an ascorbate\u2013glutathione cycle in stored broccoli (Brassica oleracea L.) florets","volume":"38","author":"Shigenaga","year":"2005","journal-title":"Postharvest Biol. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1007\/s00217-002-0560-8","article-title":"Glucosinolates and vitamin C content in edible parts of broccoli florets after domestic cooking","volume":"215","author":"Vallejo","year":"2002","journal-title":"Eur. Food Res. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/j.foodchem.2012.02.052","article-title":"Effects of processing on anthocyanins, carotenoids and vitamin C in summer fruits and vegetables","volume":"133","author":"Leong","year":"2012","journal-title":"Food chem."},{"key":"ref_26","first-page":"C336","article-title":"Thermal Stability of l-Ascorbic Acid and Ascorbic Acid Oxidase in Broccoli (Brassica oleracea var","volume":"75","author":"Munyaka","year":"2010","journal-title":"italica). J. Food Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/8\/1632\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:47:05Z","timestamp":1760186825000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/8\/1632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,25]]},"references-count":26,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2019,4]]}},"alternative-id":["molecules24081632"],"URL":"https:\/\/doi.org\/10.3390\/molecules24081632","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,25]]}}}