{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:20:16Z","timestamp":1777087216993,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Xunta da Galicia Plan I2C","award":["ED481B 2014\/086\u20130"],"award-info":[{"award-number":["ED481B 2014\/086\u20130"]}]},{"name":"Xunta da Galicia Plan I2C","award":["ED481B 2018\/007"],"award-info":[{"award-number":["ED481B 2018\/007"]}]},{"DOI":"10.13039\/100013003","name":"Universit\u00e0 degli Studi di Cagliari","doi-asserted-by":"publisher","award":["FIR"],"award-info":[{"award-number":["FIR"]}],"id":[{"id":"10.13039\/100013003","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Antibiotic resistance is one of the main public health concerns of this century. This resistance is also associated with oxidative stress, which could contribute to the selection of resistant bacterial strains. Bearing this in mind, and considering that flavonoid compounds are well known for displaying both activities, we investigated a series of hydroxy-3-arylcoumarins with structural features of flavonoids for their antibacterial activity against different bacterial strains. Active compounds showed selectivity against the studied Gram-positive bacteria compared to Gram-negative bacteria. 5,7-Dihydroxy-3-phenylcoumarin (compound 8) displayed the best antibacterial activity against Staphylococcus aureus and Bacillus cereus with minimum inhibitory concentrations (MICs) of 11 \u03bcg\/mL, followed by Staphylococcus aureus (MRSA strain) and Listeria monocytogenes with MICs of 22 and 44 \u03bcg\/mL, respectively. Moreover, molecular docking studies performed on the most active compounds against Staphylococcus aureus tyrosyl-tRNA synthetase and topoisomerase II DNA gyrase revealed the potential binding mode of the ligands to the site of the appropriate targets. Preliminary structure\u2013activity relationship studies showed that the antibacterial activity can be modulated by the presence of the 3-phenyl ring and by the position of the hydroxyl groups at the coumarin scaffold.<\/jats:p>","DOI":"10.3390\/molecules24152815","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T11:39:37Z","timestamp":1564659577000},"page":"2815","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Antibacterial Activity and Molecular Docking Studies of a Selected Series of Hydroxy-3-arylcoumarins"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6684-7795","authenticated-orcid":false,"given":"Maria Barbara","family":"Pisano","sequence":"first","affiliation":[{"name":"Department of Medical Sciences and Public Health, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2547-0071","authenticated-orcid":false,"given":"Amit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy"}]},{"given":"Rosaria","family":"Medda","sequence":"additional","affiliation":[{"name":"Department of Sciences of Life and Environment, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0615-4177","authenticated-orcid":false,"given":"Gianluca","family":"Gatto","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9083-4030","authenticated-orcid":false,"given":"Rajesh","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3009-4226","authenticated-orcid":false,"given":"Antonella","family":"Fais","sequence":"additional","affiliation":[{"name":"Department of Sciences of Life and Environment, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"given":"Benedetta","family":"Era","sequence":"additional","affiliation":[{"name":"Department of Sciences of Life and Environment, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0467-1325","authenticated-orcid":false,"given":"Sofia","family":"Cosentino","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences and Public Health, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"given":"Eugenio","family":"Uriarte","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"},{"name":"Instituto de Ciencias Qu\u00edmicas Aplicadas, Universidad Aut\u00f3noma de Chile,  Santiago 7500912, Chile"}]},{"given":"Lourdes","family":"Santana","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2350-7966","authenticated-orcid":false,"given":"Francesca","family":"Pintus","sequence":"additional","affiliation":[{"name":"Department of Sciences of Life and Environment, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3470-8299","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Matos","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/s41559-019-0854-x","article-title":"Assessing evolutionary risks of resistance for new antimicrobial therapies","volume":"3","author":"Brockhurst","year":"2019","journal-title":"Nat. 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