{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T14:58:45Z","timestamp":1775573925675,"version":"3.50.1"},"reference-count":85,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT) and the European program FEDER\/FEEI","award":["PTDC\/BTM-MAT\/28123\/2017"],"award-info":[{"award-number":["PTDC\/BTM-MAT\/28123\/2017"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT) and the European program FEDER\/FEEI","award":["PTDC\/NAN-MAT\/31036\/2017"],"award-info":[{"award-number":["PTDC\/NAN-MAT\/31036\/2017"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["MIT-EXPL\/BIO\/0089\/2017"],"award-info":[{"award-number":["MIT-EXPL\/BIO\/0089\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Freestanding films based on catechol functionalized chitosan (CHI), hyaluronic acid (HA), and bioglass nanoparticles (BGNPs) were developed by spin-coating layer-by-layer assembly (SA-LbL). The catechol groups of 3,4-dihydroxy-l-phenylalanine (DOPA) present in the marine mussels adhesive proteins (MAPs) are the main factors responsible for their characteristic strong wet adhesion. Then, the produced films were cross-linked with genipin to improve their stability in wet state. Overall, the incorporation of BGNPs resulted in thicker and bioactive films, hydrophilic and rougher surfaces, reduced swelling, higher weight loss, and lower stiffness. The incorporation of catechol groups onto the films showed a significant increase in the films\u2019 adhesion and stiffness, lower swelling, and weight loss. Interestingly, a synergetic effect on the stiffness increase was observed upon the combined incorporation of BGNPs with catechol-modified polymers, given that such films were the stiffest. Regarding the biological assays, the films exhibited no negative effects on cellular viability, adhesion, and proliferation, and the BGNPs seemed to promote higher cellular metabolic activity. These bioactive LbL freestanding films combine enhanced adhesion with improved mechanical properties and could find applications in the biomedical field, such as guided hard tissue regeneration membranes.<\/jats:p>","DOI":"10.3390\/molecules25040840","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T05:44:12Z","timestamp":1582695852000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Spin-Coated Polysaccharide-Based Multilayered Freestanding Films with Adhesive and Bioactive Moieties"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4033-3937","authenticated-orcid":false,"given":"Joana","family":"Moreira","sequence":"first","affiliation":[{"name":"3Bs Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805\u2013017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1590-6636","authenticated-orcid":false,"given":"Ana C.","family":"Vale","sequence":"additional","affiliation":[{"name":"3Bs Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805\u2013017 Guimar\u00e3es, Portugal"}]},{"given":"Ricardo","family":"A. Pires","sequence":"additional","affiliation":[{"name":"3Bs Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4680-0834","authenticated-orcid":false,"given":"Gabriela","family":"Botelho","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Minho, Campus de Gualtar, 4710\u2013057 Braga, Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3Bs Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"}]},{"given":"Nat\u00e1lia M.","family":"Alves","sequence":"additional","affiliation":[{"name":"3Bs Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Barco, 4805\u2013017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805\u2013017 Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.actbio.2017.03.003","article-title":"Design strategies and applications of nacre-based biomaterials","volume":"54","author":"Gerhard","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/s10126-007-9053-x","article-title":"Understanding marine mussel adhesion","volume":"9","author":"Silverman","year":"2007","journal-title":"Mar. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1039\/C1CS15106A","article-title":"Layered nanocomposites inspired by the structure and mechanical properties of nacre","volume":"41","author":"Wang","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7617","DOI":"10.1039\/c2ra20218b","article-title":"Hierarchical structure and mechanical properties of nacre: A review","volume":"2","author":"Sun","year":"2012","journal-title":"Rsc Adv."},{"key":"ref_5","unstructured":"Kalpana, S., Katti, D.R., and Mohanty, B. (2010). Biomimetic Lessons Learnt from Nacre. Biomimetics Learning from Nature, IntechOpen."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pmatsci.2007.05.002","article-title":"Biological materials: Structure and mechanical properties","volume":"53","author":"Meyers","year":"2008","journal-title":"Prog. Mater. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1126\/science.212.4498.1038","article-title":"Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline","volume":"212","author":"Waite","year":"1981","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1021\/bm025707n","article-title":"Effect of Oxidation Rate on Cross-Linking of Mussel Adhesive Proteins","volume":"4","author":"Haemers","year":"2003","journal-title":"Biomacromolecules"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1039\/C4PY01415D","article-title":"Mussel-inspired hydrogels for biomedical and environmental applications","volume":"6","author":"Li","year":"2015","journal-title":"Polym. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1126\/science.1147241","article-title":"Mussel-inspired surface chemistry for multifunctional coatings","volume":"318","author":"Lee","year":"2007","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.biomaterials.2016.10.005","article-title":"3D printing of biomaterials with mussel-inspired nanostructures for tumor therapy and tissue regeneration","volume":"111","author":"Ma","year":"2016","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12999","DOI":"10.1073\/pnas.0605552103","article-title":"Single-molecule mechanics of mussel adhesion","volume":"103","author":"Lee","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1126\/science.277.5330.1232","article-title":"Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites","volume":"277","author":"Decher","year":"1997","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5368","DOI":"10.1021\/ja015774+","article-title":"Multiple Membranes from \u201cTrue\u201d Polyelectrolyte Multilayers","volume":"123","author":"Dubas","year":"2001","journal-title":"J. Am. Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/(SICI)1521-3927(20000401)21:7<319::AID-MARC319>3.0.CO;2-7","article-title":"Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: Suitable materials, structure and properties","volume":"21","author":"Bertrand","year":"2000","journal-title":"Macromol. Rapid Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1002\/adma.200901327","article-title":"Multiple functionalities of polyelectrolyte multilayer films: New biomedical applications","volume":"22","author":"Boudou","year":"2010","journal-title":"Adv. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1080\/09205063.1998.9753060","article-title":"Ultrathin films of charged polysaccharides assembled alternately with linear polyions","volume":"9","author":"Lvov","year":"1998","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1021\/bm060765k","article-title":"Multifunctional Polyelectrolyte Multilayer Films:\u2009 Combining Mechanical Resistance, Biodegradability, and Bioactivity","volume":"8","author":"Schneider","year":"2007","journal-title":"Biomacromolecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cocis.2010.05.007","article-title":"Polysaccharide-based polyelectrolyte multilayers","volume":"15","author":"Crouzier","year":"2010","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1021\/cm2032459","article-title":"Polyelectrolyte Multilayer Assemblies on Materials Surfaces: From Cell Adhesion to Tissue Engineering","volume":"24","author":"Gribova","year":"2012","journal-title":"Chem. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nature02389","article-title":"High-mobility ultrathin semiconducting films prepared by spin coating","volume":"428","author":"Mitzi","year":"2004","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/adma.200306010","article-title":"Compliant, Robust, and Truly Nanoscale Free-Standing Multilayer Films Fabricated Using Spin-Assisted Layer-by-Layer Assembly","volume":"16","author":"Jiang","year":"2004","journal-title":"Adv. Mater."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Tyona, M.D. (2013). A theoritical study on spin coating technique. Adv. Mater. Res., 2.","DOI":"10.12989\/amr.2013.2.4.195"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1021\/la011333s","article-title":"Polyelectrolyte Spin-Assembly","volume":"18","author":"Chiarelli","year":"2002","journal-title":"Langmuir Acs J. Surf. Colloids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1080\/00218460590944602","article-title":"Mussel Adhesion: Finding the Tricks Worth Mimicking","volume":"81","author":"Waite","year":"2005","journal-title":"J. Adhes."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.enzmictec.2008.01.003","article-title":"Microbial transformation of geniposide in Gardenia jasminoides Ellis into genipin by Penicillium nigricans","volume":"42","author":"Xu","year":"2008","journal-title":"Enzym. Microb. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1021\/ja990469y","article-title":"Role of l-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins","volume":"121","author":"Yu","year":"1999","journal-title":"J. Am. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"75988","DOI":"10.1039\/C6RA14359H","article-title":"Biomimetic polysaccharide\/bioactive glass nanoparticles multilayer membranes for guided tissue regeneration","volume":"6","author":"Rodrigues","year":"2016","journal-title":"Rsc Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1166\/jnn.2009.389","article-title":"Nanostructured multilayer coatings combining chitosan with bioactive glass nanoparticles","volume":"9","author":"Couto","year":"2009","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1021\/acs.langmuir.5b03508","article-title":"Adhesive Bioactive Coatings Inspired by Sea Life","volume":"32","author":"Rego","year":"2016","journal-title":"Langmuir Acs J. Surf. Colloids"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.1002\/smll.201303568","article-title":"Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications","volume":"10","author":"Neto","year":"2014","journal-title":"Small"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5385","DOI":"10.1039\/C6TB00841K","article-title":"Antibacterial bioadhesive layer-by-layer coatings for orthopedic applications","volume":"4","author":"Carvalho","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Sousa, M.P., and Mano, J.F. (2017). Cell-Adhesive Bioinspired and Catechol-Based Multilayer Freestanding Membranes for Bone Tissue Engineering. Biomimetics, 2.","DOI":"10.3390\/biomimetics2040019"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1039\/C8BM00319J","article-title":"Bioinspired multilayer membranes as potential adhesive patches for skin wound healing","volume":"6","author":"Sousa","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"18904","DOI":"10.1039\/C8RA03214A","article-title":"Mussel-inspired bio-compatible free-standing adhesive films assembled layer-by-layer with water-resistance","volume":"8","author":"Wang","year":"2018","journal-title":"Rsc Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1002\/adfm.201202365","article-title":"Hyaluronic Acid Catechol: A Biopolymer Exhibiting a pH-Dependent Adhesive or Cohesive Property for Human Neural Stem Cell Engineering","volume":"23","author":"Hong","year":"2013","journal-title":"Adv. Funct. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.biomaterials.2015.02.010","article-title":"Chitosan-catechol: A polymer with long-lasting mucoadhesive properties","volume":"52","author":"Kim","year":"2015","journal-title":"Biomaterials"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.biomaterials.2014.10.024","article-title":"Genipin-crosslinked catechol-chitosan mucoadhesive hydrogels for buccal drug delivery","volume":"37","author":"Xu","year":"2015","journal-title":"Biomaterials"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1039\/C5CC08617E","article-title":"Bioinspired pH and magnetic responsive catechol-functionalized chitosan hydrogels with tunable elastic properties","volume":"52","author":"Ghadban","year":"2016","journal-title":"Chem. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15843","DOI":"10.1021\/ja0532638","article-title":"Biomimetic Anchor for Surface-Initiated Polymerization from Metal Substrates","volume":"127","author":"Fan","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1021\/bm400352d","article-title":"Bioinspired, Calcium-Free Alginate Hydrogels with Tunable Physical and Mechanical Properties and Improved Biocompatibility","volume":"14","author":"Lee","year":"2013","journal-title":"Biomacromolecules"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jiec.2015.11.015","article-title":"Biofunctionalization via flow shear stress resistant adhesive polysaccharide, hyaluronic acid-catechol, for enhanced in vitro endothelialization","volume":"34","author":"Joo","year":"2016","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5998","DOI":"10.1039\/c2cs35107b","article-title":"Layer-by-layer assembly for rapid fabrication of thick polymeric films","volume":"41","author":"Li","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0168-3659(01)00557-0","article-title":"Temperature-responsive and degradable hyaluronic acid\/Pluronic composite hydrogels for controlled release of human growth hormone","volume":"80","author":"Kim","year":"2002","journal-title":"J. Control. Release Off. J. Control. Release Soc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5349","DOI":"10.1021\/cm201801n","article-title":"Catechol-Modified Polyions in Layer-by-Layer Assembly to Enhance Stability and Sustain Release of Biomolecules: A Bioinspired Approach","volume":"23","author":"Min","year":"2011","journal-title":"Chem. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1016\/j.ceramint.2011.03.026","article-title":"Bioactive glass nanoparticles with negative zeta potential","volume":"37","author":"Doostmohammadi","year":"2011","journal-title":"Ceram. Int."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"494014","DOI":"10.1088\/0957-4484\/22\/49\/494014","article-title":"Preparation and characterization of bioactive glass nanoparticles prepared by sol-gel for biomedical applications","volume":"22","author":"Luz","year":"2011","journal-title":"Nanotechnology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1016\/S0142-9612(03)00200-X","article-title":"Bioactive sol-gel glasses with and without a hydroxycarbonate apatite layer as substrates for osteoblast cell adhesion and proliferation","volume":"24","author":"Olmo","year":"2003","journal-title":"Biomaterials"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1155\/2014\/950691","article-title":"In Vitro Bioactivity and Antimicrobial Tuning of Bioactive Glass Nanoparticles Added with Neem (Azadirachta indica) Leaf Powder","volume":"2014","author":"Prabhu","year":"2014","journal-title":"Biomed Res. Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"S53","DOI":"10.1016\/j.actbio.2015.07.019","article-title":"Reprint of: Review of bioactive glass: From Hench to hybrids","volume":"23","author":"Jones","year":"2015","journal-title":"Acta Biomater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1021\/la2004134","article-title":"Free-Standing Poly(l-lactic acid) Nanofilms Loaded with Superparamagnetic Nanoparticles","volume":"27","author":"Taccola","year":"2011","journal-title":"Langmuir Acs J. Surf. Colloids"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1039\/C5TB00082C","article-title":"Chitosan\u2013alginate multilayered films with gradients of physicochemical cues","volume":"3","author":"Silva","year":"2015","journal-title":"J. Mater. Chem. B"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4213","DOI":"10.1021\/ma991645q","article-title":"pH-Dependent Thickness Behavior of Sequentially Adsorbed Layers of Weak Polyelectrolytes","volume":"33","author":"Shiratori","year":"2000","journal-title":"Macromolecules"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1038\/s41467-017-02387-2","article-title":"Bio-inspired reversible underwater adhesive","volume":"8","author":"Zhao","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1002\/biot.201100294","article-title":"Polymeric membranes for guided bone regeneration","volume":"6","author":"Gentile","year":"2011","journal-title":"Biotechnol. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.dental.2012.04.022","article-title":"Recent advances in the development of GTR\/GBR membranes for periodontal regeneration\u2014A materials perspective","volume":"28","author":"Bottino","year":"2012","journal-title":"Dent. Mater."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"9581","DOI":"10.1021\/jp071957s","article-title":"Effect of Surface Polarity on Water Contact Angle and Interfacial Hydration Structure","volume":"111","author":"Giovambattista","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.biomaterials.2017.09.016","article-title":"A novel DOPA-albumin based tissue adhesive for internal medical applications","volume":"147","author":"Zhu","year":"2017","journal-title":"Biomaterials"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.1021\/ma101983s","article-title":"Humidity Swelling\/Deswelling Hysteresis in a Polyelectrolyte Multilayer Film","volume":"44","author":"Secrist","year":"2011","journal-title":"Macromolecules"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.cocis.2014.01.001","article-title":"Competing mechanisms in polyelectrolyte multilayer formation and swelling: Polycation\u2013polyanion pairing vs. polyelectrolyte\u2013ion pairing","volume":"19","author":"Volodkin","year":"2014","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"7725","DOI":"10.1021\/la0112099","article-title":"Swelling and Smoothing of Polyelectrolyte Multilayers by Salt","volume":"17","author":"Dubas","year":"2001","journal-title":"Langmuir Acs J. Surf. Colloids"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.1021\/cm0512225","article-title":"Correlation of the Swelling and Permeability of Polyelectrolyte Multilayer Films","volume":"17","author":"Miller","year":"2005","journal-title":"Chem. Mater."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1021\/bm200519y","article-title":"Layer-by-Layer Assembly of Polysaccharide-Based Polyelectrolyte Multilayers: A Spectroscopic Study of Hydrophilicity, Composition, and Ion Pairing","volume":"12","author":"Place","year":"2011","journal-title":"Biomacromolecules"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0009-8981(74)90398-2","article-title":"Lysozyme in human body fluids","volume":"57","author":"Hankiewicz","year":"1974","journal-title":"Clin. Chim. Acta"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"6083","DOI":"10.1021\/la904014z","article-title":"Graphene Oxide as a Matrix for Enzyme Immobilization","volume":"26","author":"Zhang","year":"2010","journal-title":"Langmuir Acs J. Surf. Colloids"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Menard, K.P. (2008). Dynamic Mechanical Analysis: A Practical Introduction, CRC Press\/Taylor & Francis Group.","DOI":"10.1201\/9781420053135"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.ijbiomac.2019.04.102","article-title":"Antibacterial free-standing polysaccharide composite films inspired by the sea","volume":"133","author":"Vale","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"8298","DOI":"10.1039\/c2sm26096d","article-title":"AFM adhesion imaging for the comparison of polyelectrolyte complexes and polyelectrolyte multilayers","volume":"8","author":"Ankerfors","year":"2012","journal-title":"Soft Matter"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/00218460008034535","article-title":"Atomic Force Microscope Techniques for Adhesion Measurements","volume":"74","author":"Schaefer","year":"2000","journal-title":"J. Adhes."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jcis.2005.05.057","article-title":"Visco-elastic and adhesive properties of adsorbed polyelectrolyte multilayers determined in situ with QCM-D and AFM measurements","volume":"292","author":"Notley","year":"2005","journal-title":"J. Colloid Interface Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1016\/j.actbio.2011.03.016","article-title":"Bioactive glass in tissue engineering","volume":"7","author":"Rahaman","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"13","DOI":"10.5402\/2012\/701695","article-title":"Deposition Mechanisms in Layer-by-Layer or Step-by-Step Deposition Methods: From Elastic and Impermeable Films to Soft Membranes with Ion Exchange Properties","volume":"2012","author":"Michel","year":"2012","journal-title":"Isrn Mater. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1002\/jbm.a.31371","article-title":"A mesoporous bioactive glass\/polycaprolactone composite scaffold and its bioactivity behavior","volume":"84A","author":"Li","year":"2008","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.colsurfb.2014.03.002","article-title":"Effects of genipin cross-linking of chitosan hydrogels on cellular adhesion and viability","volume":"117","author":"Gao","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1002\/jbm.a.30492","article-title":"Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization","volume":"75","author":"Chen","year":"2005","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.1016\/j.biomaterials.2009.05.026","article-title":"Effect of genipin cross-linking on the cellular adhesion properties of layer-by-layer assembled polyelectrolyte films","volume":"30","author":"Hillberg","year":"2009","journal-title":"Biomaterials"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1039\/b919944f","article-title":"Thermo-sensitive, injectable, and tissue adhesive sol-gel transition hyaluronic acid\/pluronic composite hydrogels prepared from bio-inspired catechol-thiol reaction","volume":"6","author":"Lee","year":"2010","journal-title":"Soft Matter"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0378-5173(98)00337-8","article-title":"Dynamic mechanical analysis of polymeric systems of pharmaceutical and biomedical significance","volume":"179","author":"Jones","year":"1999","journal-title":"Int. J. Pharm."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.surg.2009.12.009","article-title":"Sealing effect of a polysaccharide nanosheet for murine cecal puncture","volume":"148","author":"Fujie","year":"2010","journal-title":"Surgery"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.1002\/adfm.200900103","article-title":"Adhesive, Flexible, and Robust Polysaccharide Nanosheets Integrated for Tissue-Defect Repair","volume":"19","author":"Fujie","year":"2009","journal-title":"Adv. Funct. Mater."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"3549","DOI":"10.1002\/adma.200700661","article-title":"Ubiquitous Transference of a Free-Standing Polysaccharide Nanosheet with the Development of a Nano-Adhesive Plaster","volume":"19","author":"Fujie","year":"2007","journal-title":"Adv. Mater."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.triboint.2011.03.007","article-title":"Adhesion and friction of nanoparticles\/polyelectrolyte multilayer films by AFM and micro-tribometer","volume":"44","author":"Guo","year":"2011","journal-title":"Tribol. Int."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1063\/1.347181","article-title":"Growth of native oxide on a silicon surface","volume":"68","author":"Morita","year":"1990","journal-title":"J. Appl. Phys."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/adfm.200400106","article-title":"Primary Cell Adhesion on RGD-Functionalized and Covalently Crosslinked Thin Polyelectrolyte Multilayer Films","volume":"15","author":"Picart","year":"2005","journal-title":"Adv. Funct. Mater."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1016\/j.biomaterials.2006.01.017","article-title":"How useful is SBF in predicting in vivo bone bioactivity?","volume":"27","author":"Kokubo","year":"2006","journal-title":"Biomaterials"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/4\/840\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:57:50Z","timestamp":1760173070000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/4\/840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,14]]},"references-count":85,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["molecules25040840"],"URL":"https:\/\/doi.org\/10.3390\/molecules25040840","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,14]]}}}