{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T08:07:32Z","timestamp":1766390852626,"version":"build-2065373602"},"reference-count":65,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnicologia","award":["UIDB\/00100\/2020, UIDP\/00100\/2020 and PTDC\/QUI-OUT\/30988\/2017 and contract under DL No. 57\/2016 regulation"],"award-info":[{"award-number":["UIDB\/00100\/2020, UIDP\/00100\/2020 and PTDC\/QUI-OUT\/30988\/2017 and contract under DL No. 57\/2016 regulation"]}]},{"name":"FEDER (Portugal2020, Lisboa2020)","award":["LISBOA-01-0145-FEDER-030988"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-030988"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>We report herein three novel complexes whose design was based on the approach that consists of combining commercially available antibiotics with metals to attain different physicochemical properties and promote antimicrobial activity. Thus, new isostructural three-dimensional (3D) hydrogen bonding frameworks of pipemidic acid with manganese (II), zinc (II) and calcium (II) have been synthesised by mechanochemistry and are stable under shelf conditions. Notably, the antimicrobial activity of the compounds is maintained or even increased; in particular, the activity of the complexes is augmented against Escherichia coli, a representative of Gram-negative bacteria that have emerged as a major concern in drug resistance. Moreover, the synthesised compounds display similar general toxicity (Artemia salina model) levels to the original antibiotic, pipemidic acid. The increased antibacterial activity of the synthesised compounds, together with their appropriate toxicity levels, are promising outcomes.<\/jats:p>","DOI":"10.3390\/molecules25102374","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:37:38Z","timestamp":1589971058000},"page":"2374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Bioactivity of Isostructural Hydrogen Bonding Frameworks Built from Pipemidic Acid Metal Complexes"],"prefix":"10.3390","volume":"25","author":[{"given":"Paula","family":"C. Alves","sequence":"first","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural (CQE), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa (UL), Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal"},{"name":"Associa\u00e7\u00e3o do Instituto Superior T\u00e9cnico para a Investiga\u00e7\u00e3o e Desenvolvimento (IST-ID), Av. Rovisco Pais 1, 1049-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-8343","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Rijo","sequence":"additional","affiliation":[{"name":"Universidade Lus\u00f3fona\u2019s Research Center for Biosciences and Health Technologies (CBIOS), Campo Grande 376, 1749-024 Lisboa, Portugal"},{"name":"Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, Universidade de Lisboa (UL), Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal"}]},{"given":"Catarina","family":"Bravo","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural (CQE), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa (UL), Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal"},{"name":"Associa\u00e7\u00e3o do Instituto Superior T\u00e9cnico para a Investiga\u00e7\u00e3o e Desenvolvimento (IST-ID), Av. Rovisco Pais 1, 1049-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1827-7369","authenticated-orcid":false,"given":"Alexandra","family":"M. M. Antunes","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural (CQE), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa (UL), Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5599-8355","authenticated-orcid":false,"given":"V\u00e2nia","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural (CQE), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa (UL), Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal"},{"name":"Associa\u00e7\u00e3o do Instituto Superior T\u00e9cnico para a Investiga\u00e7\u00e3o e Desenvolvimento (IST-ID), Av. Rovisco Pais 1, 1049-003 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jac\/dkg212","article-title":"Development of the quinolones","volume":"51","author":"Andersson","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11153","DOI":"10.3390\/molecules180911153","article-title":"Metal Complexes of Quinolone Antibiotics and Their Applications: An Update","volume":"18","author":"Uivarosi","year":"2013","journal-title":"Molecules"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.18433\/J3302N","article-title":"Nonclassical biological activities of quinolone derivatives","volume":"15","author":"Daneshtalab","year":"2012","journal-title":"J. Pharm. Pharm. 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