{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:14:30Z","timestamp":1771978470737,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T00:00:00Z","timestamp":1593734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["POCI-01-0145-FEDER-031781"],"award-info":[{"award-number":["POCI-01-0145-FEDER-031781"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Antibiotic resistance is increasing and new strategies are needed to fight infection. Advanced materials are promising tools that can be combined with innovative alternatives to conventional antibiotics to allow more targeted and efficient treatment. In this work, we explored the activity against Staphylococcus epidermidis (S. epidermidis) of the \u03b1-helical antimicrobial peptide (AMP) MSI-78(4-20) (KFLKKAKKFGKAFVKIL) when covalently bound to a chitosan coating. The AMP MSI-78(4-20) (17 mer) is an improved version of its parent MSI-78 (22 mer; commercially known as Pexiganan), a cost-effective short AMP, which was demonstrated to be as effective as MSI-78 and less toxic to eukaryotic cells. An MSI-78(4-20)\u2013chitosan coating could be applied in several infection scenarios, ranging from bone implants to wound dressings, as chitosan possesses osteoconductive and hemostatic properties. Cysteine-modified MSI-78(4-20) was covalently immobilized onto the chitosan coating through a succinimidyl-[(N-maleimidopropionamido)-octaethyleneglycol] ester (SM(PEG)8), a heterobifuncional crosslinker, with N-hydroxysuccinimide (NHS) ester and maleimide groups, by its N- and C- termini. The MSI-78(4-20)\u2013chitosan coating demonstrated bactericidal properties independently of the tethering site and an improved performance in the presence of plasma proteins, which mimics conditions that will be encountered in vivo. This AMP\u2013chitosan coating has therefore great potential for applications in medical devices such as implants or even wound dressings.<\/jats:p>","DOI":"10.3390\/molecules25133046","type":"journal-article","created":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T11:07:42Z","timestamp":1594033662000},"page":"3046","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["AMP\u2013Chitosan Coating with Bactericidal Activity in the Presence of Human Plasma Proteins"],"prefix":"10.3390","volume":"25","author":[{"given":"Cl\u00e1udia","family":"Monteiro","sequence":"first","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"INEB, Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6391-418X","authenticated-orcid":false,"given":"H\u00e9lia","family":"Fernandes","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"INEB, Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9409-3276","authenticated-orcid":false,"given":"Diana","family":"Oliveira","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"INEB, Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1283-1042","authenticated-orcid":false,"given":"Nuno","family":"Vale","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"given":"Mariana","family":"Barbosa","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6018-4724","authenticated-orcid":false,"given":"Paula","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"given":"M. Cristina","family":"L. Martins","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"de Kraker, M.E., Stewardson, A.J., and Harbarth, S. (2016). Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050?. PLoS Med., 13.","DOI":"10.1371\/journal.pmed.1002184"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/joim.12242","article-title":"Microbial biofilm formation: A need to act","volume":"276","author":"Romling","year":"2014","journal-title":"J. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1007\/s00253-018-8776-0","article-title":"Strategies to prevent the occurrence of resistance against antibiotics by using advanced materials","volume":"102","author":"Bassegoda","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1016\/j.actbio.2010.11.005","article-title":"Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces","volume":"7","author":"Costa","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1177\/0022034516679973","article-title":"Antimicrobial Peptides: Mechanisms of Action and Resistance","volume":"96","author":"Bechinger","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.1021\/acs.molpharmaceut.5b00113","article-title":"A 17-mer Membrane-Active MSI-78 Derivative with Improved Selectivity toward Bacterial Cells","volume":"12","author":"Monteiro","year":"2015","journal-title":"Mol. Pharm."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ijbiomac.2018.01.006","article-title":"Chitosan: An undisputed bio-fabrication material for tissue engineering and bio-sensing applications","volume":"110","author":"Baranwal","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1111\/j.1365-2672.2008.03942.x","article-title":"A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses","volume":"105","author":"Willcox","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"129","DOI":"10.2741\/s453","article-title":"Chemical immobilization of antimicrobial peptides on biomaterial surfaces","volume":"8","author":"Silva","year":"2016","journal-title":"Front. Biosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1021\/acs.langmuir.7b03668","article-title":"Monitoring Antimicrobial Mechanisms of Surface-Immobilized Peptides in Situ","volume":"34","author":"Xiao","year":"2018","journal-title":"Langmuir"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.1021\/jp4122003","article-title":"Different interfacial behaviors of peptides chemically immobilized on surfaces with different linker lengths and via different termini","volume":"118","author":"Han","year":"2014","journal-title":"J. Phys. Chem. B"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1002\/psc.2787","article-title":"Immobilization and orientation-dependent activity of a naturally occurring antimicrobial peptide","volume":"21","author":"Soares","year":"2015","journal-title":"J. Pept. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7146","DOI":"10.1021\/jp5125487","article-title":"Effects of Peptide Immobilization Sites on the Structure and Activity of Surface-Tethered Antimicrobial Peptides","volume":"119","author":"Li","year":"2015","journal-title":"J. Phys. Chem. C"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3513","DOI":"10.1016\/j.actbio.2014.02.028","article-title":"Characterization of hLF1-11 immobilization onto chitosan ultrathin films, and its effects on antimicrobial activity","volume":"10","author":"Costa","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.biomaterials.2015.02.049","article-title":"Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption","volume":"52","author":"Costa","year":"2015","journal-title":"Biomaterials"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"28182","DOI":"10.1039\/C7CP05445A","article-title":"Repelling and ordering: The influence of poly(ethylene glycol) on protein adsorption","volume":"19","author":"Bernhard","year":"2017","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2878","DOI":"10.1021\/acs.langmuir.6b04481","article-title":"Effect of Linker Length on Cell Capture by Poly(ethylene glycol)-Immobilized Antimicrobial Peptides","volume":"33","author":"Anderson","year":"2017","journal-title":"Langmuir"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/j.1751-7915.2008.00080.x","article-title":"Chitosan and its antimicrobial potential\u2014A critical literature survey","volume":"2","author":"Raafat","year":"2009","journal-title":"Microb. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.ijbiomac.2017.11.119","article-title":"Enhanced Antibacterial effect of chitosan film using Montmorillonite\/CuO nanocomposite","volume":"109","author":"Nouri","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.ijbiomac.2017.04.076","article-title":"Incorporation of lysozyme-rectorite composites into chitosan films for antibacterial properties enhancement","volume":"102","author":"Li","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"17374","DOI":"10.1038\/s41598-017-17310-4","article-title":"N-acetylcysteine-functionalized coating avoids bacterial adhesion and biofilm formation","volume":"7","author":"Costa","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.actbio.2018.12.001","article-title":"Antimicrobial coatings prepared from Dhvar-5-click-grafted chitosan powders","volume":"84","author":"Barbosa","year":"2019","journal-title":"Acta Biomater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1016\/j.peptides.2011.05.023","article-title":"Inhibitory effects and mechanisms of physiological conditions on the activity of enantiomeric forms of an alpha-helical antibacterial peptide against bacteria","volume":"32","author":"Huang","year":"2011","journal-title":"Peptides"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0001-8686(86)80012-4","article-title":"Adsorption of proteins from solution at the solid-liquid interface","volume":"25","author":"Norde","year":"1986","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1002\/jbm.a.10096","article-title":"Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers","volume":"67","author":"Martins","year":"2003","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5208","DOI":"10.1016\/j.actbio.2012.09.011","article-title":"Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer","volume":"9","author":"Nogueira","year":"2013","journal-title":"Acta Biomater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3814","DOI":"10.1021\/la702653m","article-title":"Interactions between chitosan and SDS at a low-charged silica substrate compared to interactions in the bulk--the effect of ionic strength","volume":"24","author":"Lundin","year":"2008","journal-title":"Langmuir"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jcis.2010.06.019","article-title":"Self-assembled monolayers of silver nanoparticles firmly grafted on glass surfaces: Low Ag+ release for an efficient antibacterial activity","volume":"350","author":"Pallavicini","year":"2010","journal-title":"J. Colloid Interface Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/13\/3046\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:47:02Z","timestamp":1760176022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/13\/3046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,3]]},"references-count":28,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["molecules25133046"],"URL":"https:\/\/doi.org\/10.3390\/molecules25133046","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,3]]}}}