{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:29:40Z","timestamp":1772252980805,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Maslinic acid (MA) is a natural triterpene from Olea europaea L. with multiple biological properties. The aim of the present study was to examine MA\u2019s effect on cell viability (by the MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key antioxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to those on healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 \u00b5M), whereas no effect was found in A10 cells treated with MA (up to 210 \u00b5M). In order to produce a stress situation in cells, 0.15 mM H2O2 was added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, which were higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in antioxidant enzyme activities depending on the cell line. In A10 cells, all the enzymes were up-regulated, but in B16F10 cells, only superoxide dismutase, glutathione S-transferase and glutathione peroxidase increased their activities. MA restored the enzyme activities to levels similar to those in the control group in both cell lines, highlighting that in A10 cells, the highest MA doses induced values lower than control. Overall, these findings demonstrate the great antioxidant capacity of MA.<\/jats:p>","DOI":"10.3390\/molecules25174020","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T11:22:43Z","timestamp":1599132163000},"page":"4020","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Unveiling the Differential Antioxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment with Hydrogen Peroxide"],"prefix":"10.3390","volume":"25","author":[{"given":"Khalida","family":"Mokhtari","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Avenida Fuentenueva, s\/n, 18071 Granada, Spain"},{"name":"Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, Mohammed I University of Oujda, Oujda 60000, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9971-8623","authenticated-orcid":false,"given":"Amalia","family":"P\u00e9rez-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Sciences, University of Granada, Avenida Fuentenueva, s\/n, 18071 Granada, Spain"}]},{"given":"Leticia","family":"Garc\u00eda-Salguero","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Avenida Fuentenueva, s\/n, 18071 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-7145","authenticated-orcid":false,"given":"Jos\u00e9","family":"A. Lupi\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Avenida Fuentenueva, s\/n, 18071 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9650-825X","authenticated-orcid":false,"given":"Eva E.","family":"Rufino-Palomares","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Avenida Fuentenueva, s\/n, 18071 Granada, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.2174\/0929867322666141212122921","article-title":"Oleanolic acid and related triterpenoids from olives on vascular function: Molecular mechanisms and therapeutic perspectives","volume":"22","year":"2015","journal-title":"Curr. Med. 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