{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:18:30Z","timestamp":1760239110998,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T00:00:00Z","timestamp":1602460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","award":["TEAM TECH CORE FACILITY\/2017-4\/6"],"award-info":[{"award-number":["TEAM TECH CORE FACILITY\/2017-4\/6"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["PZ00P3_174169"],"award-info":[{"award-number":["PZ00P3_174169"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004442","name":"Narodowym Centrum Nauki","doi-asserted-by":"publisher","award":["UMO-2014\/12\/W\/NZ1\/00457"],"award-info":[{"award-number":["UMO-2014\/12\/W\/NZ1\/00457"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2012\/07\/E\/NZ1\/01907"],"award-info":[{"award-number":["2012\/07\/E\/NZ1\/01907"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["Leading National Research Center (KNOW) - Jagiellonian University"],"award-info":[{"award-number":["Leading National Research Center (KNOW) - Jagiellonian University"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IF\/00656\/2014","SFRH\/BDP\/79243\/2011"],"award-info":[{"award-number":["IF\/00656\/2014","SFRH\/BDP\/79243\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Streptococcus pneumoniae is a frequent bacterial pathogen of the human respiratory tract causing pneumonia, meningitis and sepsis, a serious healthcare burden in all age groups. S. pneumoniae lacks complete respiratory chain and relies on carbohydrate fermentation for energy generation. One of the essential components for this includes the mannose phosphotransferase system (Man-PTS), which plays a central role in glucose transport and exhibits a broad specificity for a range of hexoses. Importantly, Man-PTS is involved in the global regulation of gene expression for virulence determinants. We herein report the three-dimensional structure of the EIIA domain of S. pneumoniae mannose phosphotransferase system (SpEIIA-Man). Our structure shows a dimeric arrangement of EIIA and reveals a detailed molecular description of the active site. Since PTS transporters are exclusively present in microbes and sugar transporters have already been suggested as valid targets for antistreptococcal antibiotics, our work sets foundation for the future development of antimicrobial strategies against Streptococcus pneumoniae.<\/jats:p>","DOI":"10.3390\/molecules25204633","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T07:23:22Z","timestamp":1602919402000},"page":"4633","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Crystal Structure of Mannose Specific IIA Subunit of Phosphotransferase System from Streptococcus pneumoniae"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-5913","authenticated-orcid":false,"given":"Malgorzata","family":"Magoch","sequence":"first","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"},{"name":"Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8040-7753","authenticated-orcid":false,"given":"Przemyslaw","family":"Nogly","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, ITQB NOVA, 2780-157 Oeiras, Portugal"},{"name":"Institute of Molecular Biology and Biophysics, Department of Biology, ETH Z\u00fcrich, Wolfgang-Pauli-Strasse 27, 8093 Z\u00fcrich, Switzerland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7157-0014","authenticated-orcid":false,"given":"Przemyslaw","family":"Grudnik","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4080-2908","authenticated-orcid":false,"given":"Pikyee","family":"Ma","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, ITQB NOVA, 2780-157 Oeiras, Portugal"},{"name":"Laboratory of Biomolecular Research, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5946-844X","authenticated-orcid":false,"given":"Bozena","family":"Boczkus","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"},{"name":"Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"}]},{"given":"Ana Rute","family":"Neves","sequence":"additional","affiliation":[{"name":"Bacterial Physiology, R&amp;D, Chr. Hansen A\/S, B\u00f8ge All\u00e9 10-12, 2970 H\u00f8rsholm, Denmark"}]},{"given":"Margarida","family":"Archer","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, ITQB NOVA, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5535-5760","authenticated-orcid":false,"given":"Grzegorz","family":"Dubin","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00092","DOI":"10.1128\/mBio.00092-11","article-title":"Streptococcus pneumoniae is desiccation tolerant and infectious upon rehydration","volume":"2","author":"Walsh","year":"2011","journal-title":"MBio"},{"key":"ref_2","first-page":"a010215","article-title":"The Pneumococcus: Epidemiology, microbiology, and pathogenesis","volume":"3","author":"Tuomanen","year":"2013","journal-title":"Cold Spring Harb. 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