{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:03:07Z","timestamp":1776132187593,"version":"3.50.1"},"reference-count":91,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/138187\/2018"],"award-info":[{"award-number":["SFRH\/BD\/138187\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BAA-AGR\/31798\/2017"],"award-info":[{"award-number":["PTDC\/BAA-AGR\/31798\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundo de Desenvolvimento Regional (FEDER) - Norte2020","award":["Actin\u00eddea-NORTE-01-0247-FEDER-033647"],"award-info":[{"award-number":["Actin\u00eddea-NORTE-01-0247-FEDER-033647"]}]},{"name":"Horizon 2020","award":["857251"],"award-info":[{"award-number":["857251"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pseudomonas syringae pv. actinidiae (Psa) is the pathogenic agent responsible for the bacterial canker of kiwifruit (BCK) leading to major losses in kiwifruit productions. No effective treatments and measures have yet been found to control this disease. Despite antimicrobial peptides (AMPs) having been successfully used for the control of several pathogenic bacteria, few studies have focused on the use of AMPs against Psa. In this study, the potential of six AMPs (BP100, RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) to control Psa was investigated. The minimal inhibitory and bactericidal concentrations (MIC and MBC) were determined and membrane damaging capacity was evaluated by flow cytometry analysis. Among the tested AMPs, the higher inhibitory and bactericidal capacity was observed for BP100 and CA-M with MIC of 3.4 and 3.4\u20136.2 \u00b5M, respectively and MBC 3.4\u201310 \u00b5M for both. Flow cytometry assays suggested a faster membrane permeation for peptide 3.1, in comparison with the other AMPs studied. Peptide mixtures were also tested, disclosing the high efficiency of BP100:3.1 at low concentration to reduce Psa viability. These results highlight the potential interest of AMP mixtures against Psa, and 3.1 as an antimicrobial molecule that can improve other treatments in synergic action.<\/jats:p>","DOI":"10.3390\/molecules26051461","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T12:12:18Z","timestamp":1615205538000},"page":"1461","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1695-2762","authenticated-orcid":false,"given":"Nuno","family":"Mariz-Ponte","sequence":"first","affiliation":[{"name":"Biology Department, Faculty of Science, University of Porto (FCUP), 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Biology Department, Faculty of Science (FCUP), University of Porto, 4169-007 Porto, Portugal"},{"name":"CIBIO\u2014Research Centre in Biodiversity and Genetic Resources, In-BIO-Associate Laboratory, Microbial Diversity and Evolution Group, University of Porto (UP), 4485-661 Vair\u00e3o, Portugal"}]},{"given":"Laura","family":"Regalado","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, University of Porto (FCUP), 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Biology Department, Faculty of Science (FCUP), University of Porto, 4169-007 Porto, Portugal"}]},{"given":"Emil","family":"Gimranov","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, University of Porto (FCUP), 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Biology Department, Faculty of Science (FCUP), University of Porto, 4169-007 Porto, Portugal"}]},{"given":"Nat\u00e1lia","family":"Tassi","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences (FCUP), University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6465-081X","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Moura","sequence":"additional","affiliation":[{"name":"CISAS\u2014Centre for Research and Development in Agrifood Systems and Sustainability, Instituto Polit\u00e9cnico de Viana do Castelo, 4900-347 Viana do Castelo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6018-4724","authenticated-orcid":false,"given":"Paula","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences (FCUP), University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9913-1155","authenticated-orcid":false,"given":"Fernando","family":"Tavares","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, University of Porto (FCUP), 4169-007 Porto, Portugal"},{"name":"CIBIO\u2014Research Centre in Biodiversity and Genetic Resources, In-BIO-Associate Laboratory, Microbial Diversity and Evolution Group, University of Porto (UP), 4485-661 Vair\u00e3o, Portugal"}]},{"given":"Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, University of Porto (FCUP), 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Biology Department, Faculty of Science (FCUP), University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9506-3781","authenticated-orcid":false,"given":"C\u00e1tia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences (FCUP), University of Porto, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00248-019-01459-8","article-title":"Pseudomonas syringae pv. actinidiae: Ecology, infection dynamics and disease epidemiology","volume":"80","author":"Donati","year":"2020","journal-title":"Microb. 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