{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:53:09Z","timestamp":1760151189605,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Important scientific areas, such as cellular biology, medicine, pharmacy, and environmental sciences, are dependent on very sensitive analytical techniques to track and detect biomolecules. In this work, we develop a simple, low-cost and effective synthetic strategy to produce new red-shifted 4-styrylcoumarin derivatives as promising inexpensive fluorescent labels for biomolecules. The extension of the delocalized \u03c0-electron system results in bathochromic shifts in these new coumarin derivatives, which also present large Stokes shifts. In addition, density functional theory and time-dependent density functional theory calculations helped to rationalize the photophysical properties observed by the experimental results.<\/jats:p>","DOI":"10.3390\/molecules27051461","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T22:34:30Z","timestamp":1645569270000},"page":"1461","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules"],"prefix":"10.3390","volume":"27","author":[{"given":"Raquel","family":"Eust\u00e1quio","sequence":"first","affiliation":[{"name":"Hercules Laboratory, University of \u00c9vora, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4419-6431","authenticated-orcid":false,"given":"Jo\u00e3o P. Prates","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Chemistry Department, School of Sciences and Technology, University of \u00c9vora, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"},{"name":"Laqv-Requimte, University of \u00c9vora, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5409-6990","authenticated-orcid":false,"given":"Ana T.","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Hercules Laboratory, University of \u00c9vora, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"},{"name":"Chemistry Department, School of Sciences and Technology, University of \u00c9vora, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]},{"given":"Ant\u00f3nio","family":"Pereira","sequence":"additional","affiliation":[{"name":"Hercules Laboratory, University of \u00c9vora, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"},{"name":"Chemistry Department, School of Sciences and Technology, University of \u00c9vora, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/978-1-4939-7271-5_6","article-title":"Fluorescent Labeling of Proteins","volume":"1665","author":"Modesti","year":"2018","journal-title":"Methods Mol. 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