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In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP\/SS and CaP\/SS\/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.<\/jats:p>","DOI":"10.3390\/molecules27072249","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:28:39Z","timestamp":1648675719000},"page":"2249","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Continuous Production of Highly Tuned Silk\/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-9629","authenticated-orcid":false,"given":"Anabela","family":"Veiga","sequence":"first","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Laboratory for Process Engineering, Environment, Biotechnology & Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE-Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Rui","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"given":"Marta M.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-1069","authenticated-orcid":false,"given":"Juliana R.","family":"Dias","sequence":"additional","affiliation":[{"name":"ALiCE-Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5016-0868","authenticated-orcid":false,"given":"Nuno M.","family":"Alves","sequence":"additional","affiliation":[{"name":"Centre for Rapid and Sustainable Product Development, Instituto Polit\u00e9cnico de Leiria, 2430-028 Marinha Grande, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-8539","authenticated-orcid":false,"given":"Ana Rita","family":"Costa-Pinto","sequence":"additional","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal"},{"name":"IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Rua J\u00falio Amaral de Carvalho 45, 4200-135 Porto, Portugal"}]},{"given":"Filipa","family":"Castro","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology & Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE-Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2778-8374","authenticated-orcid":false,"given":"Fernando","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology & Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE-Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Ana L.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102353","DOI":"10.1016\/j.nano.2020.102353","article-title":"New Prospects in Skin Regeneration and Repair Using Nanophased Hydroxyapatite Embedded in Collagen Nanofibers","volume":"33","author":"Ribeiro","year":"2021","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1046\/j.1524-475X.2002.10502.x","article-title":"Calcium: A Potential Central Regulator in Wound Healing in the Skin","volume":"10","author":"Lansdown","year":"2002","journal-title":"Wound Repair Regen."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"110400","DOI":"10.1016\/j.msec.2019.110400","article-title":"Hydroxyapatite\/Sericin Composites: A Simple Synthesis Route under near-Physiological Conditions of Temperature and PH and Preliminary Study of the Effect of Sericin on the Biomineralization Process","volume":"108","author":"Veiga","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmats.2020.00024","article-title":"Recent Advances in Silk Sericin\/Calcium Phosphate Biomaterials","volume":"7","author":"Veiga","year":"2020","journal-title":"Front. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1163\/156856209X453006","article-title":"The Effect of Sericin with Variable Amino-Acid Content from Different Silk Strains on the Production of Collagen and Nitric Oxide","volume":"20","author":"Aramwit","year":"2009","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1023\/A:1023993400608","article-title":"Sericin, a Protein Derived from Silkworms, Accelerates the Proliferation of Several Mammalian Cell Lines Including a Hybridoma","volume":"40","author":"Terada","year":"2002","journal-title":"Cytotechnology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1021\/bm401740x","article-title":"Biomimetic Nucleation of Hydroxyapatite Crystals Mediated by Antheraea Pernyi Silk Sericin Promotes Osteogenic Differentiation of Human Bone Marrow Derived Mesenchymal Stem Cells","volume":"15","author":"Yang","year":"2014","journal-title":"Biomacromolecules"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sunarintyas, S., and Siswomihardjo, W. (2011, January 8\u20139). The Effect of Sericin Application over Hydroxyapatite Surface on Osteoblast Cells Proliferation. Proceedings of the  International Conference on Instrumentation, Communication, Information Technology and Biomedical Engineering, Bandung, Indonesia.","DOI":"10.1109\/ICICI-BME.2011.6108613"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5751","DOI":"10.1039\/b901620a","article-title":"Effect of Silk Sericin on Assembly of Hydroxyapatite Nanocrystals into Enamel Prism-like Structure","volume":"19","author":"Cai","year":"2009","journal-title":"J. Mater. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Vieira, E., Silva, M., Maia-Filho, A., Ferreira, D., Figuer\u00eado-Silva, J., Rovaris, K., Fialho, A.C., Leite-Oliveira, A., de Oliveira, A.L.M., and da Fonseca, M.G. (2021). Effect of Cerium-Containing Hydroxyapatite in Bone Repair in Female Rats with Osteoporosis Induced by Ovariectomy. Minerals, 11.","DOI":"10.3390\/min11040377"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1016\/j.biomaterials.2012.11.061","article-title":"Effects of Cerium Oxide Nanoparticles on the Growth of Keratinocytes, Fibroblasts and Vascular Endothelial Cells in Cutaneous Wound Healing","volume":"34","author":"Chigurupati","year":"2013","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"075404","DOI":"10.1088\/2053-1591\/3\/7\/075404","article-title":"Continuous-Flow Precipitation as a Route to Prepare Highly Controlled Nanohydroxyapatite: In Vitro Mineralization and Biological Evaluation","volume":"3","author":"Castro","year":"2016","journal-title":"Mater. Res. Express"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/jctb.6532","article-title":"High Efficient Strategy for the Production of Hydroxyapatite\/Silk Sericin Nanocomposites","volume":"96","author":"Veiga","year":"2021","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1021\/acs.iecr.0c04991","article-title":"Mixing Performance of Planar Oscillatory Flow Reactors with Liquid Solutions and Solid Suspensions","volume":"60","author":"Cruz","year":"2021","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4483","DOI":"10.1002\/aic.14193","article-title":"Continuous-Flow Precipitation of Hydroxyapatite at 37 \u00b0C in a Meso Oscillatory Flow Reactor","volume":"59","author":"Castro","year":"2013","journal-title":"AIChE J."},{"key":"ref_16","unstructured":"Veiga, A., Castro, F., Magalh\u00e3es, R., Rocha, F., and Oliveira, A.L. (2021, January 5\u20139). Fabrication of Highly Tuned Calcium Phosphate\/Silk Sericin Composites: Exploring New Pathways on Skin Regeneration. Proceedings of the 31st Conference of the European Society For Biomaterials, Porto, Portugal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1002\/jbm.10280","article-title":"Synthesis and Characterization of Hydroxyapatite Crystals: A Review Study on the Analytical Methods","volume":"62","author":"Koutsopoulos","year":"2002","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1039\/C2TB00153E","article-title":"A Novel Method of Forming Micro- and Macroporous Monetite Cements","volume":"1","author":"Cama","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2041731414536572","article-title":"In Vitro Osteoinductive Potential of Porous Monetite for Bone Tissue Engineering","volume":"5","author":"Idowu","year":"2014","journal-title":"J. Tissue Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2934","DOI":"10.1021\/cm500650t","article-title":"Infrared Absorption Spectrum of Brushite from First Principles","volume":"26","author":"Hirsch","year":"2014","journal-title":"Chem. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.1016\/S0955-2219(03)00304-2","article-title":"Carbonated Hydroxyapatite as Bone Substitute","volume":"23","author":"Landi","year":"2003","journal-title":"J. Eur. Ceram. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8572","DOI":"10.1021\/acsomega.9b04421","article-title":"DFT-Calculated IR Spectrum Amide I, II, and III Band Contributions of N-Methylacetamide Fine Components","volume":"5","author":"Ji","year":"2020","journal-title":"ACS Omega"},{"key":"ref_23","unstructured":"RRUFF (2021, May 25). Brushite R070554. Available online: https:\/\/rruff.info\/Brushite."},{"key":"ref_24","unstructured":"RRUFF (2021, May 25). Hydroxylapatite R050512. Available online: https:\/\/rruff.info\/hydrox\/display=default\/R050512."},{"key":"ref_25","first-page":"405","article-title":"Precipitation of Hydroxyapatite at 37o C in a Meso Oscillatory Flow Reactor Operated in Batch at Constant Power Density","volume":"7","author":"Castro","year":"2014","journal-title":"AIChE J."},{"key":"ref_26","first-page":"13","article-title":"Hydroxyapatite-Based Materials: Synthesis and Characterization","volume":"Volume 1","year":"2011","journal-title":"Biomedical Engineering\u2014Frontiers and Challenges"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.rinp.2018.08.012","article-title":"Structural Characterization of Cerium-Doped Hydroxyapatite Nanoparticles Synthesized by an Ultrasonic-Assisted Sol-Gel Technique","volume":"10","author":"Phatai","year":"2018","journal-title":"Results Phys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.mattod.2015.10.008","article-title":"Calcium Phosphates in Biomedical Applications: Materials for the Future?","volume":"19","author":"Habraken","year":"2016","journal-title":"Mater. Today"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s10856-005-6981-8","article-title":"Synthesis and Structure of Cerium-Substituted Hydroxyapatite","volume":"6","author":"Feng","year":"2005","journal-title":"J. Mater. Sci. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1080\/01932690701728924","article-title":"A Novel Continuous Industrial Process for Producing Hydroxyapatite Nanoparticles","volume":"29","author":"Silva","year":"2008","journal-title":"J. Dispers. Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.jallcom.2017.07.116","article-title":"Spray Drying of Hydroxyapatite Powders: The Effect of Spray Drying Parameters and Heat Treatment on the Particle Size and Morphology","volume":"724","author":"Bastan","year":"2017","journal-title":"J. Alloys Compd."},{"key":"ref_32","unstructured":"Elliot, J.C. (1994). Struture and Chemistry of the Apatites and Other Calcium Orthophosphates, Elsevier. [1st ed.]."},{"key":"ref_33","first-page":"201","article-title":"Crystallisation","volume":"6","author":"Mullin","year":"2002","journal-title":"Org. Process Res. Dev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/(SICI)1521-4079(1998)33:2<197::AID-CRAT197>3.0.CO;2-K","article-title":"Crystallization and Characterization of Calcium Phosphates: Brushite and Monetite","volume":"33","author":"Sivkumar","year":"1998","journal-title":"Cryst. Res. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1016\/S0142-9612(01)00358-1","article-title":"Crystallinity and Solubility Characteristics of Hydroxyapatite Adsorbed Amino Acid","volume":"23","author":"Matsumoto","year":"2002","journal-title":"Biomaterials"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.ultsonch.2006.12.004","article-title":"Ultrasound-Assisted Crystallization (Sonocrystallization)","volume":"14","author":"Castro","year":"2007","journal-title":"Ultrason. Sonochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.ces.2013.01.002","article-title":"Process Intensification and Optimization for Hydroxyapatite Nanoparticles Production","volume":"100","author":"Castro","year":"2013","journal-title":"Chem. Eng. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s11706-012-0154-8","article-title":"The Role of Crystallinity on Differential Attachment\/Proliferation of Osteoblasts and Fibroblasts on Poly (Caprolactone-Co-Glycolide) Polymeric Surfaces","volume":"6","author":"Cui","year":"2012","journal-title":"Front. Mater. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1007\/s12195-015-0422-7","article-title":"Heading in the Right Direction: Understanding Cellular Orientation Responses to Complex Biophysical Environments","volume":"9","author":"Tamiello","year":"2016","journal-title":"Cell. Mol. Bioeng."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9284","DOI":"10.1038\/s41598-017-09430-8","article-title":"Cerium Oxide Nanoparticles Inhibit Differentiation of Neural Stem Cells","volume":"7","author":"Gliga","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1111\/jcmm.13685","article-title":"Actin Cytoskeleton Assembly Regulates Collagen Production via TGF-\u03b2 Type II Receptor in Human Skin Fibroblasts","volume":"22","author":"Qin","year":"2018","journal-title":"J. Cell. Mol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Eliaz, N., and Metoki, N. (2017). Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials, 10.","DOI":"10.3390\/ma10040334"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/j.actbio.2008.04.003","article-title":"Brushite\u2014Collagen Composites for Bone Regeneration","volume":"4","author":"Tamimi","year":"2008","journal-title":"Acta Biomater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s13204-015-0509-4","article-title":"Effect of Preparation Conditions on the Nanostructure of Hydroxyapatite and Brushite Phases","volume":"6","author":"Mansour","year":"2016","journal-title":"Appl. Nanosci."},{"key":"ref_45","first-page":"23","article-title":"Influence of Hydroxyapatite Crystallinity on the Growth of Keratinocytes","volume":"109","author":"Smetana","year":"2008","journal-title":"Prague Med. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"31516","DOI":"10.1038\/srep31516","article-title":"Systematic Evaluation of Sericin Protein as a Substitute for Fetal Bovine Serum in Cell Culture","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/jat.1745","article-title":"Effects of Four Types of Hydroxyapatite Nanoparticles with Different Nanocrystal Morphologies and Sizes on Apoptosis in Rat Osteoblasts","volume":"32","author":"Xu","year":"2012","journal-title":"J. Appl. Toxicol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mocanu, A.C., Stan, G.E., Maidaniuc, A., Miculescu, M., Antoniac, I.V., Ciocoiu, R.C., Voicu, \u015e.I., Mitran, V., C\u00eempean, A., and Miculescu, F. (2019). Naturally-Derived Biphasic Calcium Phosphates through Increased Phosphorus-Based Reagent Amounts for Biomedical Applications. Materials, 12.","DOI":"10.3390\/ma12030381"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"462","DOI":"10.3389\/fbioe.2019.00462","article-title":"Bioactivity of Biphasic Calcium Phosphate Granules, the Control of a Needle-Like Apatite Layer Formation for Further Medical Device Developments","volume":"7","author":"Rouillon","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"E49","DOI":"10.1111\/tid.12046","article-title":"Fatal Escherichia Coli Skin and Soft Tissue Infections in Liver Transplant Recipients: Report of Three Cases","volume":"15","author":"Janny","year":"2013","journal-title":"Transpl. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1016\/j.ceramint.2019.10.104","article-title":"Development of Composites Scaffolds with Calcium and Cerium-Hydroxyapatite and Gellan Gum","volume":"46","author":"Santos","year":"2020","journal-title":"Ceram. Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1049\/iet-nbt.2017.0029","article-title":"Development and Characterisation of Novel Ce-Doped Hydroxyapatite\u2013Fe3O4 Nanocomposites and Their in Vitro Biological Evaluations for Biomedical Applications","volume":"12","author":"Baskaran","year":"2018","journal-title":"IET Nanobiotechnology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"12192","DOI":"10.1016\/j.ceramint.2015.06.040","article-title":"New Cerium(IV)-Substituted Hydroxyapatite Nanoparticles: Preparation and Characterization","volume":"41","author":"Ciobanu","year":"2015","journal-title":"Ceram. Int."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e27106","DOI":"10.1371\/journal.pone.0027106","article-title":"Calcium-Based Nanoparticles Accelerate Skin Wound Healing","volume":"6","author":"Kawai","year":"2011","journal-title":"PLoS ONE"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Mathew-Steiner, S.S., Roy, S., and Sen, C.K. (2021). Collagen in Wound Healing. Bioengineering, 8.","DOI":"10.3390\/bioengineering8050063"},{"key":"ref_56","unstructured":"WOUNDS (2021, August 02). The Use of Novel New Multi-tissue Biomaterial Powder Focused on Cell Signaling in Wound Healing Applications: A Case Series. Available online: https:\/\/www.hmpgloballearningnetwork.com\/site\/wounds\/poster\/use-novel-new-multi-tissue-biomaterial-powder-focused-cell-signaling-wound-healing."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e5421","DOI":"10.1371\/journal.pone.0005421","article-title":"Characterization of Granulations of Calcium and Apatite in Serum as Pleomorphic Mineralo-Protein Complexes and as Precursors of Putative Nanobacteria","volume":"4","author":"Young","year":"2009","journal-title":"PLoS ONE"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1016\/B978-0-12-374144-8.00241-1","article-title":"Sericulture","volume":"Volume 1","author":"Takeda","year":"2009","journal-title":"Encyclopedia of Insects"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"364","DOI":"10.3892\/br.2014.244","article-title":"Antioxidant Activities of Two Sericin Proteins Extracted from Cocoon of Silkworm (Bombyx Mori) Measured by DPPH, Chemiluminescence, ORAC and ESR Methods","volume":"2","author":"Takechi","year":"2014","journal-title":"Biomed. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0734-9750(02)00003-4","article-title":"Applications of Natural Silk Protein Sericin in Biomaterials","volume":"20","author":"Zhang","year":"2002","journal-title":"Biotechnol. Adv."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4948","DOI":"10.1016\/j.actbio.2012.10.025","article-title":"Expanded Applications, Shifting Paradigms and an Improved Understanding of Host\u2013Biomaterial Interactions","volume":"9","author":"Brown","year":"2013","journal-title":"Acta Biomater."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e16683","DOI":"10.1002\/aic.16683","article-title":"The Axial Dispersion of Liquid Solutions and Solid Suspensions in Planar Oscillatory Flow Crystallizers","volume":"65","author":"Cruz","year":"2019","journal-title":"AIChE J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1021\/acsbiomaterials.0c01745","article-title":"In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial","volume":"7","author":"Borges","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Angel, R., and Petrova, E. (2021, July 26). Quant-iTTMPicoGreen\u00ae dsDNA Assay Kit (ThermoFisher). Available online: https:\/\/www.protocols.io\/view\/qant-it-picogreen-dsdna-quantification-bftfjnjn?step=12.","DOI":"10.17504\/protocols.io.b2etqben"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2249\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:46:45Z","timestamp":1760136405000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/7\/2249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,30]]},"references-count":64,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["molecules27072249"],"URL":"https:\/\/doi.org\/10.3390\/molecules27072249","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,30]]}}}