{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:28:25Z","timestamp":1772774905030,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gio (CNPq)","doi-asserted-by":"publisher","award":["560714\/2010-6"],"award-info":[{"award-number":["560714\/2010-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gio (CNPq)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gio (CNPq)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","doi-asserted-by":"publisher","award":["560714\/2010-6"],"award-info":[{"award-number":["560714\/2010-6"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Universidade Federal do Estado do Rio de Janeiro (UNIRIO)","doi-asserted-by":"publisher","award":["560714\/2010-6"],"award-info":[{"award-number":["560714\/2010-6"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Universidade Federal do Estado do Rio de Janeiro (UNIRIO)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Universidade Federal do Estado do Rio de Janeiro (UNIRIO)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF). A factorial design was performed to determine the influence variables and two rotational central composite designs were executed. The validated experimental result was of 7.1 U mL\u22121 using 50% PFRF (w\/w), pH 5, 30 \u00b0C for 24 h, under static SSF. Polygalacturonase, pectin methyl esterase, pectin\u2013lyase and pectate\u2013lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL\u22121, respectively. Shotgun proteomics analysis of the crude extract enabled the identification of two pectin\u2013lyases, one pectate\u2013lyase and a glucosidase. The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 \u00b0C, respectively, over 60 min incubation. Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases. Statistical optimization led to fermentation conditions wherein pectin active proteins predominated. To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.<\/jats:p>","DOI":"10.3390\/molecules27154981","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T22:33:11Z","timestamp":1659997991000},"page":"4981","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach"],"prefix":"10.3390","volume":"27","author":[{"given":"Matheus Mikio","family":"Takeyama","sequence":"first","affiliation":[{"name":"Food and Nutrition Graduate Program (PPGAN), Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"}]},{"given":"M\u00e1rcia Corr\u00eaa","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Food and Nutrition Graduate Program (PPGAN), Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"}]},{"given":"Helena Sacco","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Nutrition School, Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6306-4998","authenticated-orcid":false,"given":"Cristiane Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Food and Nutrition Graduate Program (PPGAN), Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3992-8474","authenticated-orcid":false,"given":"Ana Paula Trovatti","family":"Uetanabaro","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Santa Cruz State University (UESC), Ilh\u00e9us 45662-900, BA, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2823-1305","authenticated-orcid":false,"given":"Andrea Miura","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Santa Cruz State University (UESC), Ilh\u00e9us 45662-900, BA, Brazil"}]},{"given":"Joseph A. Medeiros","family":"Evaristo","sequence":"additional","affiliation":[{"name":"Laboratory of Proteomics\/LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-598, RJ, Brazil"}]},{"given":"F\u00e1bio C\u00e9sar Sousa","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Laboratory of Proteomics\/LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Proteomics Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8594-2667","authenticated-orcid":false,"given":"Ana Elizabeth Cavalcante","family":"Fai","sequence":"additional","affiliation":[{"name":"Food and Nutrition Graduate Program (PPGAN), Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"},{"name":"Department of Basic and Experimental Nutrition, Rio de Janeiro State University (UERJ), Rio de Janeiro 20550-013, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5558-570X","authenticated-orcid":false,"given":"Maria Gabriela Bello","family":"Koblitz","sequence":"additional","affiliation":[{"name":"Food and Nutrition Graduate Program (PPGAN), Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro 22290-240, RJ, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.ijbiomac.2018.09.048","article-title":"Recent advances in the production strategies of microbial pectinases\u2014A review","volume":"122","author":"Amin","year":"2019","journal-title":"Int. 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