{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:50:23Z","timestamp":1762066223295,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T00:00:00Z","timestamp":1662422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["SFRH\/BD\/149360\/2019","DL 57\/2016","UIDB\/00709\/2020","UIDP\/00709\/2020"],"award-info":[{"award-number":["SFRH\/BD\/149360\/2019","DL 57\/2016","UIDB\/00709\/2020","UIDP\/00709\/2020"]}]},{"name":"CICS-UBI","award":["SFRH\/BD\/149360\/2019","DL 57\/2016","UIDB\/00709\/2020","UIDP\/00709\/2020"],"award-info":[{"award-number":["SFRH\/BD\/149360\/2019","DL 57\/2016","UIDB\/00709\/2020","UIDP\/00709\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ayahuasca is an Amazonian drink, which contains \u03b2-carboline alkaloids and N,N-dimethyltryptamine. The aim of this study was to evaluate the healing potential of decoctions of a commercial mixture, four individual plants and four mixtures of two plants used in the ayahuasca preparation. Thus, the cytotoxic potential of the samples was evaluated and a wound-healing assay was performed with a NHDF cell line. Subsequently, a parallel artificial membrane permeability assay was also performed, to verify if any psychoactive compound could be absorbed by skin fibroblasts. The integrity and permeability of the cell layer were also evaluated, using the transepithelial electrical resistance assay and Lucifer yellow permeability assay, respectively. The compounds absorbed by the cell layer were quantified by high-performance liquid chromatography coupled to a diode array detector. The results showed that only one sample showed cytotoxicity and all the others promoted the migration of skin fibroblasts. Additionally, it was also verified that \u03b2-carbolynic alkaloids and N,N-dimethyltriptamine were not absorbed by the cell layer, and in general, did not interfere with its permeability and integrity. To the best of our knowledge, this is the first study where ayahuasca\u2019s wound-healing potential was evaluated.<\/jats:p>","DOI":"10.3390\/molecules27185760","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T01:51:57Z","timestamp":1662515517000},"page":"5760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the In Vitro Wound-Healing Potential of Ayahuasca"],"prefix":"10.3390","volume":"27","author":[{"given":"Joana","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade (CICS-UBI), Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Laborat\u00f3rio de F\u00e1rmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0712-6522","authenticated-orcid":false,"given":"\u00c2ngelo","family":"Lu\u00eds","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade (CICS-UBI), Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Laborat\u00f3rio de F\u00e1rmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1802-8998","authenticated-orcid":false,"given":"Eugenia","family":"Gallardo","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade (CICS-UBI), Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Laborat\u00f3rio de F\u00e1rmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3333-5977","authenticated-orcid":false,"given":"Ana Paula","family":"Duarte","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade (CICS-UBI), Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Laborat\u00f3rio de F\u00e1rmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102206","DOI":"10.1016\/j.tmaid.2021.102206","article-title":"Ayahuasca and the traveller: A scoping review of risks and possible benefits","volume":"44","author":"Houle","year":"2021","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, J., Lu\u00eds, \u00c2., Gradillas, A., Garc\u00eda, A., Restolho, J., Fern\u00e1ndez, N., Domingues, F., Gallardo, E., and Duarte, A.P. (2020). Ayahuasca Beverages: Phytochemical Analysis and Biological Properties. Antibiotics, 9.","DOI":"10.3390\/antibiotics9110731"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, J., Lu\u00eds, \u00c2., Gallardo, E., and Duarte, A.P. (2021). Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples. Molecules, 26.","DOI":"10.3390\/molecules26051397"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"898","DOI":"10.3389\/fpsyt.2021.687615","article-title":"Ceremonial Ayahuasca in Amazonian Retreats-Mental Health and Epigenetic Outcomes from a Six-Month Naturalistic Study","volume":"12","author":"Ruffell","year":"2021","journal-title":"Front. Psychiatry"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.4155\/bio-2020-0148","article-title":"Recent bionalytical methods for the determination of new psychoactive substances in biological specimens","volume":"12","author":"Antunes","year":"2020","journal-title":"Bioanalysis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1590\/1516-4446-2020-0913","article-title":"Risk assessment of ayahuasca use in a religious context: Self-reported risk factors and adverse effects","volume":"43","author":"Durante","year":"2021","journal-title":"Rev. Bras. Psiquiatr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.9740\/mhc.2017.01.039","article-title":"Ayahuasca: An ancient sacrament for treatment of contemporary psychiatric illness?","volume":"7","author":"Malcolm","year":"2017","journal-title":"Ment. Health Clin."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"748131","DOI":"10.3389\/fpsyg.2021.748131","article-title":"The Power of Social Attribution: Perspectives on the Healing Efficacy of Ayahuasca","volume":"12","year":"2021","journal-title":"Front. Psychol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, J., Castilho, M., Rosado, T., Lu\u00eds, \u00c2., Restolho, J., Fern\u00e1ndez, N., Gallardo, E., and Duarte, A.P. (2021). In Vitro Study of the Bioavailability and Bioaccessibility of the Main Compounds Present in Ayahuasca Beverages. Molecules, 26.","DOI":"10.3390\/molecules26185555"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.pharmthera.2004.03.002","article-title":"Clinical investigations of the therapeutic potential of ayahuasca: Rationale and regulatory challenges","volume":"102","author":"Mckenna","year":"2004","journal-title":"Pharmacol. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11207","DOI":"10.1073\/pnas.1902174116","article-title":"Chemical evidence for the use of multiple psychotropic plants in a 1,000-year-old ritual bundle from South America","volume":"116","author":"Miller","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1590\/1516-4446-2018-0140","article-title":"Acute effects of ayahuasca in a juvenile non-human primate model of depression","volume":"41","author":"Silva","year":"2019","journal-title":"Braz. J. Psychiatry"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/02791072.2009.10400530","article-title":"A six-month prospective evaluation of personality traits, psychiatric symptoms and quality of life in ayahuasca-na\u00efve subjects","volume":"41","author":"Barbosa","year":"2009","journal-title":"J. Psychoact. Drugs"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1017\/S0033291718001356","article-title":"Rapid antidepressant effects of the psychedelic ayahuasca in treatment-resistant depression: A randomized placebo-controlled trial","volume":"49","author":"Barreto","year":"2019","journal-title":"Psychol. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1590\/1516-4446-2014-1496","article-title":"Antidepressant effects of a single dose of ayahuasca in patients with recurrent depression: A preliminary report","volume":"37","author":"Sanches","year":"2015","journal-title":"Rev. Bras. Psiquiatr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/02791072.2005.10399801","article-title":"Altered States of Consciousness and Short-Term Psychological After-Effects Induced by the First Time Ritual Use of Ayahuasca in an Urban Context in Brazil","volume":"37","author":"Barbosa","year":"2011","journal-title":"J. Psychoact. Drugs"},{"key":"ref_17","first-page":"129","article-title":"Ayahuasca in Adolescence: A Preliminary Psychiatric Assessment","volume":"37","author":"Grob","year":"2011","journal-title":"J. Psychoact. Drugs"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Afonso, A.M., Gon\u00e7alves, J., Lu\u00eds, \u00c2., Gallardo, E., and Duarte, A.P. (2020). Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey. Appl. Sci., 10.","DOI":"10.3390\/app10051845"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cjtee.2018.10.004","article-title":"The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum","volume":"22","author":"Felician","year":"2019","journal-title":"Chin. J. Traumatol. = Zhonghua chuang shang za zhi"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Santos, E.S., Lu\u00eds, \u00c2., Gon\u00e7alves, J., Rosado, T., Pereira, L., Gallardo, E., and Duarte, A.P. (2020). Julbernardia paniculata and Pterocarpus angolensis: From Ethnobotanical Surveys to Phytochemical Characterization and Bioactivities Evaluation. Molecules, 25.","DOI":"10.3390\/molecules25081828"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"113003","DOI":"10.1016\/j.bbr.2020.113003","article-title":"Anti-inflammatory activity of ayahuasca: Therapeutical implications in neurological and psychiatric diseases","volume":"400","author":"Daros","year":"2021","journal-title":"Behav. Brain Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-12779-5","article-title":"Short term changes in the proteome of human cerebral organoids induced by 5-meo-DMT","volume":"7","author":"Dakic","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_23","first-page":"469","article-title":"Analysis of Cell Viability by the MTT Assay","volume":"2018","author":"Kumar","year":"2018","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.jep.2009.10.030","article-title":"Banisteriopsis caapi, a unique combination of MAO inhibitory and antioxidative constituents for the activities relevant to neurodegenerative disorders and Parkinson\u2019s disease","volume":"127","author":"Samoylenko","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"112743","DOI":"10.1016\/j.jep.2020.112743","article-title":"Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: Applicability to Parkinson\u2019s disease","volume":"255","author":"Santos","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Santos, B.W.L., Moreira, D.C., Borges, T.K.D.S., and Caldas, E.D. (2022). Components of Banisteriopsis caapi, a Plant Used in the Preparation of the Psychoactive Ayahuasca, Induce Anti-Inflammatory Effects in Microglial Cells. Molecules, 27.","DOI":"10.3390\/molecules27082500"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sim\u00e3o, A.Y., Gon\u00e7alves, J., Gradillas, A., Garc\u00eda, A., Restolho, J., Fern\u00e1ndez, N., Rodilla, J.M., Barroso, M., Duarte, A.P., and Crist\u00f3v\u00e3o, A.C. (2020). Evaluation of the Cytotoxicity of Ayahuasca Beverages. Molecules, 25.","DOI":"10.3390\/molecules25235594"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jaim.2016.11.007","article-title":"Studies on wound healing potential of polyherbal formulation using in vitro and in vivo assays","volume":"8","author":"Talekar","year":"2017","journal-title":"J. Ayurveda Integr. Med."},{"key":"ref_29","first-page":"46","article-title":"Transepithelial\/endothelial Electrical Resistance (TEER) theory and ap-plications for microfluidic body-on-a-chip devices Keywords TEER Body-on-a-chip Barrier tissue Blood-brain barrier Organ Endothelial cells Epithelial cells Human-on-a-chip","volume":"1","author":"Elbrecht","year":"2016","journal-title":"J. Rare Dis Res. Treat."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1039\/c3fo60099h","article-title":"Evaluation of a possible direct effect by casein phosphopeptides on paracellular and vitamin D controlled transcellular calcium transport mechanisms in intestinal human HT-29 and Caco2 cell lines","volume":"4","author":"Colombini","year":"2013","journal-title":"Food Funct."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1023\/A:1008136103927","article-title":"The changes in the neuronal PC12 and the intestinal epithelial Caco-2 cells during the coculture. The functional analysis using an in vitro coculture system","volume":"35","author":"Satsu","year":"2001","journal-title":"Cytotechnology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1523\/JNEUROSCI.04-10-02477.1984","article-title":"Decrease of gap junction permeability induced by dopamine and cyclic adenosine 3\u2032:5\u2032-monophosphate in horizontal cells of turtle retina","volume":"4","author":"Piccolino","year":"1984","journal-title":"J. Neurosci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/18\/5760\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:24:34Z","timestamp":1760142274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/18\/5760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,6]]},"references-count":32,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["molecules27185760"],"URL":"https:\/\/doi.org\/10.3390\/molecules27185760","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2022,9,6]]}}}