{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T00:57:47Z","timestamp":1768093067783,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Portuguese \u201cFunda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\u201d (FCT, Lisbon)","award":["UIDB\/000081\/2020"],"award-info":[{"award-number":["UIDB\/000081\/2020"]}]},{"name":"The Portuguese \u201cFunda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\u201d (FCT, Lisbon)","award":["LA\/P\/0056\/2020"],"award-info":[{"award-number":["LA\/P\/0056\/2020"]}]},{"name":"Associated Laboratory IMS","award":["UIDB\/000081\/2020"],"award-info":[{"award-number":["UIDB\/000081\/2020"]}]},{"name":"Associated Laboratory IMS","award":["LA\/P\/0056\/2020"],"award-info":[{"award-number":["LA\/P\/0056\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The present work was designed to investigate the effects of different extraction processes, namely ultrasonic-assisted, supercritical fluid, microwave-assisted and Soxhlet applied to carob pods. The total phenolic quantification and the antioxidant activity were assessed by the means of rapid in vitro spectrophotometric assays; the phenolic profile was identified using ultra-high performance liquid chromatography coupled to mass spectrometry. The results revealed that the phenolic compounds and the antioxidant capacity varied significantly with the nature of the extraction process. The content of total phenolic compounds ranged from 11.55 to 34.38 mg GAE\/g DW; the content of total flavonoids varied from 3.50 to 10.53 mg QE\/g DW, and the content of condensed tannins fluctuated from 3.30 to 6.55 mg CE\/ g DW. All extracts performed differently on antioxidant activity when determined by the DPPH assay producing a dose-dependent response, with IC50 extended from 11.33 to 6.07 \u00b5g\/mL. HPLC analysis enabled the identification of nine compounds. As a function of the studied extraction methods, the phenolic compound contents were positively correlated with antioxidant activity.<\/jats:p>","DOI":"10.3390\/molecules27196163","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T22:36:44Z","timestamp":1663713404000},"page":"6163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Evaluation of Different Extraction Methods on the Phenolic Profile and the Antioxidant Potential of Ceratonia siliqua L. Pods Extracts"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8112-1474","authenticated-orcid":false,"given":"Fouad El","family":"Mansouri","sequence":"first","affiliation":[{"name":"Research Team: Materials, Environment and Sustainable Development (MEDD), Faculty of Sciences and Techniques of Tangier, Abdelmalek Essa\u00e2di University, Tangier BP 416, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-3441","authenticated-orcid":false,"given":"Joaquim C. G. Esteves","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Qu\u00edmica (CIQUP), Instituto de Ci\u00eancias Moleculares (IMS), Departamento de Geoci\u00eancias, Ambiente e Ordenamento do Territ\u00f3rio, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-7633","authenticated-orcid":false,"given":"Francesco","family":"Cacciola","sequence":"additional","affiliation":[{"name":"Department of Biomedical Dental Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy"}]},{"given":"Fadoua","family":"Asraoui","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biology and Pathologies, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essa\u00e2di University, Tetouan 93000, Morocco"}]},{"given":"Hatim","family":"Tayeq","sequence":"additional","affiliation":[{"name":"MAE2D Laboratory, Polydisciplinary Faculty of Larache, Abdelmalek Essa\u00e2di University, Tetouan 93000, Morocco"}]},{"given":"Yasmine Mttougui","family":"Ben Amar","sequence":"additional","affiliation":[{"name":"Research Team: Materials, Environment and Sustainable Development (MEDD), Faculty of Sciences and Techniques of Tangier, Abdelmalek Essa\u00e2di University, Tangier BP 416, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8509-4226","authenticated-orcid":false,"given":"Miguel Palma","family":"Lovillo","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigaci\u00f3n Vitivin\u00edcola y Agroalimentaria (IVAGRO), University of Cadiz, Campus del Rio San Pedro, 11510 Cadiz, Spain"}]},{"given":"Noureddine","family":"Chouaibi","sequence":"additional","affiliation":[{"name":"Laboratory of LAMSE, Faculty of Sciences and Techniques of Tangier, Tangier BP 416, Morocco"}]},{"given":"Jamal","family":"Brigui","sequence":"additional","affiliation":[{"name":"Research Team: Materials, Environment and Sustainable Development (MEDD), Faculty of Sciences and Techniques of Tangier, Abdelmalek Essa\u00e2di University, Tangier BP 416, Morocco"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mariod, A.A. 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