{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:50:11Z","timestamp":1762300211112,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,23]],"date-time":"2022-10-23T00:00:00Z","timestamp":1666483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"],"award-info":[{"award-number":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"],"award-info":[{"award-number":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"],"award-info":[{"award-number":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"]}]},{"name":"LEAF-Linking Landscape, Environment, Agriculture and Food, Research Unit","award":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"],"award-info":[{"award-number":["SFRH\/BD\/140829\/201","SFRH\/BD\/131947\/2017","UIDP\/04378\/2020","LA\/P\/0140\/2020","UIDB\/04129\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>FucoPol is an acylated polysaccharide with demonstrated valuable functional properties that include a shear thinning fluid behaviour, a film-forming capacity, and an emulsion forming and stabilizing capacity. In this study, the different conditions (concentration, temperature, and time) for alkaline treatment were investigated to deacylate FucoPol. Complete deacetylation and desuccinylation was achieved with 0.02 M NaOH, at 60 \u00b0C for 15 min, with no significant impact on the biopolymer\u2019s sugar composition, pyruvate content, and molecular mass distribution. FucoPol depyruvylation by acid hydrolysis was attempted, but it resulted in a very low polymer recovery. The effect of the ionic strength, pH, and temperature on the deacetylated\/desuccinylated polysaccharide, d-FucoPol, was evaluated, as well as its emulsion and film-forming capacity. d-FucoPol aqueous solutions maintained the shear thinning behaviour characteristic of FucoPol, but the apparent viscosity decreased significantly. Moreover, contrary to FucoPol, whose solutions were not affected by the media\u2019s ionic strength, the d-FucoPol solutions had a significantly higher apparent viscosity for a higher ionic strength. On the other hand, the d-FucoPol solutions were not affected by the pH in the range of 3.6\u201311.5, while FucoPol had a decreased viscosity for acidic pH values and for a pH above 10.5. Although d-FucoPol displayed an emulsification activity for olive oil similar to that of FucoPol (98 \u00b1 0%) for an oil-to-water ratio of 2:3, the emulsions were less viscous. The d-FucoPol films were flexible, with a higher Young\u2032s modulus (798 \u00b1 152 MPa), a stress at the break (22.5 \u00b1 2.5 MPa), and an elongation at the break (9.3 \u00b1 0.7%) than FucoPol (458 \u00b1 32 MPa, 15.5 \u00b1 0.3 MPa and 8.1 \u00b1 1.0%, respectively). Given these findings, d-FucoPol arises as a promising novel biopolymer, with distinctive properties that may render it useful for utilization as a suspending or emulsifier agent, and as a barrier in coatings and packaging films.<\/jats:p>","DOI":"10.3390\/molecules27217165","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T08:19:06Z","timestamp":1666599546000},"page":"7165","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol: Impact on Biopolymer Physical and Chemical Properties"],"prefix":"10.3390","volume":"27","author":[{"given":"S\u00edlvia","family":"Baptista","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"73100, Lda. Edif\u00edcio Arcis, Rua Ivone Silva, 6, 4\u00b0 piso, 1050-124 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4376-6471","authenticated-orcid":false,"given":"Diana","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"i3N\/CENIMAT, Department of Materials Science, Faculty of Sciences and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, 2829-516 Almada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6143-9170","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Conc\u00f3rdio-Reis","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6878-1040","authenticated-orcid":false,"given":"Ana C.","family":"Marques","sequence":"additional","affiliation":[{"name":"i3N\/CENIMAT, Department of Materials Science, Faculty of Sciences and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, 2829-516 Almada, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"i3N\/CENIMAT, Department of Materials Science, Faculty of Sciences and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, 2829-516 Almada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4117-5582","authenticated-orcid":false,"given":"V\u00edtor D.","family":"Alves","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-4640","authenticated-orcid":false,"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Almada, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.carbpol.2010.07.034","article-title":"Fucose-Containing Exopolysaccharide Produced by the Newly Isolated Enterobacter strain A47 DSM 23139","volume":"83","author":"Freitas","year":"2011","journal-title":"Carbohydr. 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