{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:02:49Z","timestamp":1762074169494,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT; Lisbon, Portugal)","doi-asserted-by":"publisher","award":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"],"award-info":[{"award-number":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT through a fellowship","doi-asserted-by":"publisher","award":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"],"award-info":[{"award-number":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Stimulus of Scientific Employment Program","doi-asserted-by":"publisher","award":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"],"award-info":[{"award-number":["PTDC\/CAL-ZOO\/30238\/2017","UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/04129\/2020","SFRH\/BPD\/116816\/2016","DL57\/2016\/CP1438\/CT0007"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The effect of a high incorporation level of Ulva lactuca, individually and supplemented with a Carbohydrate-Active enZyme (CAZyme) on broilers\u2019 plasma parameters and liver composition is assessed here. Twenty one-day-old Ross 308 male broilers were randomly assigned to one of four treatments (n = 10): corn\/soybean meal based-diet (Control); based-diet with 15% U. lactuca (UL); UL diet with 0.005% of commercial carbohydrase mixture; and UL diet with 0.01% of recombinant ulvan lyase. Supplementing U. lactuca with the recombinant CAZyme slightly compromised broilers\u2019 growth by negatively affecting final body weight and average daily gain. The combination of U. lactuca with ulvan lyase also increased systemic lipemia through an increase in total lipids, triacylglycerols and VLDL-cholesterol (p &lt; 0.001). Moreover, U. lactuca, regardless of the CAZyme supplementation, enhanced hepatic n-3 PUFA (mostly 20:5n-3) with positive decrease in n-6\/n-3 ratio. However, broilers fed with U. lactuca with ulvan lyase reduced hepatic \u03b1- and \u03b3-tocopherol concentrations relative to the control. Conversely, the high amount of pigments in macroalga diets led to an increase in hepatic \u03b2-carotene, chlorophylls and total carotenoids. Furthermore, U. lactuca, alone or combined with CAZymes, enhanced hepatic total microminerals, including iron and manganese. Overall, plasma metabolites and liver composition changed favorably in broilers that were fed 15% of U. lactuca, regardless of enzyme supplementation.<\/jats:p>","DOI":"10.3390\/molecules27217425","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T08:15:12Z","timestamp":1667376912000},"page":"7425","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Plasma Metabolites and Liver Composition of Broilers in Response to Dietary Ulva lactuca with Ulvan Lyase or a Commercial Enzyme Mixture"],"prefix":"10.3390","volume":"27","author":[{"given":"Cristina M.","family":"Alfaia","sequence":"first","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3030-7789","authenticated-orcid":false,"given":"M\u00f3nica M.","family":"Costa","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1667-7871","authenticated-orcid":false,"given":"Rui M. A.","family":"Pinto","sequence":"additional","affiliation":[{"name":"iMed.UL, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal"},{"name":"JCS-Laborat\u00f3rio de An\u00e1lises Cl\u00ednicas Dr. Joaquim Chaves, Rua An\u00edbal Bettencourt, n\u00b0 3, Outurela, 2790-224 Carnaxide, Portugal"}]},{"given":"Jos\u00e9 M.","family":"Pestana","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4843-1064","authenticated-orcid":false,"given":"Miguel","family":"Mourato","sequence":"additional","affiliation":[{"name":"LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"given":"Patr\u00edcia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1017-3269","authenticated-orcid":false,"given":"C\u00e1tia F.","family":"Martins","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6755-4572","authenticated-orcid":false,"given":"Paula A.","family":"Lopes","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"}]},{"given":"Madalena M.","family":"Lordelo","sequence":"additional","affiliation":[{"name":"LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-5987","authenticated-orcid":false,"given":"Jos\u00e9 A. M.","family":"Prates","sequence":"additional","affiliation":[{"name":"CIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"},{"name":"Laborat\u00f3rio Associado para Ci\u00eancia Animal e Veterin\u00e1ria (AL4AnimalS), Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"ref_1","unstructured":"(2021). Meat, OECD-FAO Agricultural Outlook 2021\u20132030."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1111\/jpn.13509","article-title":"Current knowledge and future perspectives of the use of seaweeds for livestock production and meat quality: A systematic review","volume":"105","author":"Costa","year":"2021","journal-title":"J. Anim. Physiol. Anim. 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