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Amongst several known fluorophores, 3-difluoroborodipyrromethene (BODIPY) derivatives have become a cornerstone for innovative fluorescent labelling applications, mainly due to their advantageous features including their facile synthesis, structural versatility and exceptional photophysical properties. In this context, we report a BODIPY-based fluorescent probe for imaging of lysosomes in living cells. The BODIPY derivative displayed a remarkable fluorescence enhancement at low pH values with a pKa* of 3.1. In vitro studies by confocal microscopy in HeLa cells demonstrated that the compound was able to permeate cell membrane and selectively label lysosome whilst remaining innocuous to the cell culture at the maximum concentration tested. Herein, the BODIPY derivative holds the promise of investigating lysosomal dynamics and function in living cells through fluorescence imaging.<\/jats:p>","DOI":"10.3390\/molecules27228065","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T04:33:32Z","timestamp":1669005212000},"page":"8065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Bioimaging of Lysosomes with a BODIPY pH-Dependent Fluorescent Probe"],"prefix":"10.3390","volume":"27","author":[{"given":"Raquel C. R.","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, International Iberian Nanotechnology Laboratory, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"given":"Efres","family":"Belmonte-Reche","sequence":"additional","affiliation":[{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, International Iberian Nanotechnology Laboratory, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1848-1167","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Pina","sequence":"additional","affiliation":[{"name":"Coimbra Chemistry Centre-Institute of Molecular Sciences, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6951-2504","authenticated-orcid":false,"given":"Milene","family":"Costa da Silva","sequence":"additional","affiliation":[{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, International Iberian Nanotechnology Laboratory, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"given":"S\u00f3nia C. S.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-3234","authenticated-orcid":false,"given":"Juan","family":"Gallo","sequence":"additional","affiliation":[{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, International Iberian Nanotechnology Laboratory, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7915-4720","authenticated-orcid":false,"given":"Susana P. G.","family":"Costa","sequence":"additional","affiliation":[{"name":"Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7996-1626","authenticated-orcid":false,"given":"M. Manuela M.","family":"Raposo","sequence":"additional","affiliation":[{"name":"Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.1021\/cr900263j","article-title":"New Strategies for Fluorescent Probe Design in Medical Diagnostic Imaging","volume":"110","author":"Kobayashi","year":"2010","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13641","DOI":"10.1039\/C8CC08107G","article-title":"Advances in the Design of Cell-Permeable Fluorescent Probes for Applications in Live Cell Imaging","volume":"54","author":"Alamudi","year":"2018","journal-title":"Chem. 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