{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T06:29:29Z","timestamp":1775024969221,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Northern Regional Operational Program","award":["NORTE 2020"],"award-info":[{"award-number":["NORTE 2020"]}]},{"name":"Northern Regional Operational Program","award":["NORTE-08-5369-FSE-000028"],"award-info":[{"award-number":["NORTE-08-5369-FSE-000028"]}]},{"name":"Portugal 2020 and the European Social Fund (ESF)","award":["NORTE 2020"],"award-info":[{"award-number":["NORTE 2020"]}]},{"name":"Portugal 2020 and the European Social Fund (ESF)","award":["NORTE-08-5369-FSE-000028"],"award-info":[{"award-number":["NORTE-08-5369-FSE-000028"]}]},{"name":"Chemical and Biological Engineering (PDEQB)","award":["NORTE 2020"],"award-info":[{"award-number":["NORTE 2020"]}]},{"name":"Chemical and Biological Engineering (PDEQB)","award":["NORTE-08-5369-FSE-000028"],"award-info":[{"award-number":["NORTE-08-5369-FSE-000028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Bioactive compounds from olive mill pomace (OMP) were extracted through a two-step solid-liquid extraction procedure considering four factors at five levels of a central composite rotatable response surface design. The influence of the process variables time of the primary extraction (2.0\u20134.0 h), solvent-to-sample ratio during the primary extraction (5.0\u201310.0 mL\/g), time of the secondary extraction (1.0\u20132.0 h), and the solvent-to-sample ratio during the secondary extraction (3.0\u20135.0 mL\/g) were examined. The content of bioactive compounds was determined spectrophotometrically, and the individual phenolic compounds were evaluated by reserved-phase high-performance liquid chromatography (RP-HPLC). The Derringer\u2019s function was used to optimize the extraction process, and the best conditions were found to be 3.2 h for the primary extraction, 10.0 mL\/g for the solvent-to-sample ratio and 1.3 h for the secondary extraction associated with a solvent-to-sample ratio of 3.0 mL\/g, obtaining a total phenolic content of 50.0 (expressed as mg gallic acid equivalents (GAE)\/g dry weight (dw). The response surface methodology proved to be a great alternative for reducing the number of tests, allowing the optimization of the extraction of phenolic antioxidants from OMP with a reduced number of experiments, promoting reductions in cost and analysis time.<\/jats:p>","DOI":"10.3390\/molecules27238620","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T04:38:54Z","timestamp":1670387934000},"page":"8620","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Response Surface Modeling and Optimization of the Extraction of Phenolic Antioxidants from Olive Mill Pomace"],"prefix":"10.3390","volume":"27","author":[{"given":"Filipa","family":"Paulo","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2112-4702","authenticated-orcid":false,"given":"Loleny","family":"Tavares","sequence":"additional","affiliation":[{"name":"ESAN\u2014School of Design, Management and Production Technologies Northern Aveiro, University of Aveiro, Estrada do Cercal 449, Oliveira de Azem\u00e9is, 3720-509 Santiago de Riba-Ul, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-0622","authenticated-orcid":false,"given":"L\u00facia","family":"Santos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.3390\/nu9101063","article-title":"Adherence to Mediterranean Diet and Risk of Cancer: An Updated Systematic Review and Meta-Analysis","volume":"9","author":"Schwingshackl","year":"2017","journal-title":"Nutrients"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1080\/10408398.2020.1748563","article-title":"Deriving valorization of phenolic compounds from olive oil by-products for food applications through microencapsulation approaches: A comprehensive review","volume":"61","author":"Paulo","year":"2021","journal-title":"Crit. 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