{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T23:01:42Z","timestamp":1777762902385,"version":"3.51.4"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P., Portugal","award":["UIDB\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P., Portugal","award":["UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDP\/04129\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food Research Centre","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food Research Centre","doi-asserted-by":"publisher","award":["UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDP\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, low-calorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with medium-chain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05\u201328.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 \u00b0C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30\u201360 and the specific productivity ranged 0.96\u20131.87 g NewTAG\/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining.<\/jats:p>","DOI":"10.3390\/molecules28062637","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T06:57:11Z","timestamp":1678777031000},"page":"2637","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils"],"prefix":"10.3390","volume":"28","author":[{"given":"Joana","family":"Souza-Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Instituto Superior de Agronomia, Universidade de Lisboa, LEAF\u2014Linking Landscape, Environment, Agriculture and Food\u2014Research Center, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-5787","authenticated-orcid":false,"given":"Ars\u00e9nio","family":"Fialho","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal"},{"name":"iBB\u2014Institute for Bioengineering and Biosciences and i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5932-9842","authenticated-orcid":false,"given":"Cleide M. F.","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (ITP), Avenida Murilo Dantas 300\u2014Farolandia, Aracaju 49032-490, Brazil"},{"name":"Tiradentes University (UNIT), Avenida Murilo Dantas 300\u2014Farolandia, Aracaju 49032-490, Brazil"}]},{"given":"Nat\u00e1lia M.","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico de Set\u00fabal, Escola Superior de Tecnologia do Barreiro, 2839-001 Lavradio, Portugal"},{"name":"Instituto Superior de Agronomia, Universidade de Lisboa, Centro de Estudos Florestais, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3655-8201","authenticated-orcid":false,"given":"Suzana","family":"Ferreira-Dias","sequence":"additional","affiliation":[{"name":"Instituto Superior de Agronomia, Universidade de Lisboa, LEAF\u2014Linking Landscape, Environment, Agriculture and Food\u2014Research Center, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal"},{"name":"Instituto Superior de Agronomia, Universidade de Lisboa, Laborat\u00f3rio de Estudos T\u00e9cnicos, 1349-017 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"ref_1","unstructured":"(2023, January 10). 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