{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T15:29:44Z","timestamp":1774020584881,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\/MCTES (Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior, Portugal)","award":["EXPL\/SAU-NUT\/0370\/2021"],"award-info":[{"award-number":["EXPL\/SAU-NUT\/0370\/2021"]}]},{"name":"FCT\/MCTES (Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior, Portugal)","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"FCT\/MCTES (Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior, Portugal)","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}]},{"name":"FCT\/MCTES (Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior, Portugal)","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]},{"name":"European Regional Development Fund","award":["EXPL\/SAU-NUT\/0370\/2021"],"award-info":[{"award-number":["EXPL\/SAU-NUT\/0370\/2021"]}]},{"name":"European Regional Development Fund","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"European Regional Development Fund","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}]},{"name":"European Regional Development Fund","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Olive pomace is a by-product from olive oil production that can be further processed to obtain olive pomace paste. In this work, the influence of different time\/temperature binomials (65 \u00b0C\/30 min; 77 \u00b0C\/1 min; 88 \u00b0C\/15 s; and 120 \u00b0C\/20 min) on the nutritional quality, chemical composition, and efficiency on control\/elimination of natural microbial load of olive pomace paste was ascertained. The treatments significantly impacted the contents of ash, fat, vitamin E, phenolics (including hydroxytyrosol), flavonoids, and antioxidant activity, but not the fatty acids profile. The binomial 88 \u00b0C\/15 s showed the greatest potential since it better preserved the phytochemical and antioxidant properties as well as the protein and fiber contents. This binomial is also faster and easy to be implemented at an industrial level, allowing the obtention of a safe functional ingredient to satisfy consumers\u2019 demands for novel sustainable products, simultaneously, responding to food safety and food security concerns.<\/jats:p>","DOI":"10.3390\/molecules28062876","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T02:35:26Z","timestamp":1679538926000},"page":"2876","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Different Time\/Temperature Binomials on the Chemical Features, Antioxidant Activity, and Natural Microbial Load of Olive Pomace Paste"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-4968","authenticated-orcid":false,"given":"Maria Manuela","family":"Sousa","sequence":"first","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0774-2168","authenticated-orcid":false,"given":"Diana Melo","family":"Ferreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4567-0687","authenticated-orcid":false,"given":"Susana","family":"Machado","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Joana C.","family":"Lobo","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Anabela S. G.","family":"Costa","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-9942","authenticated-orcid":false,"given":"Josman D.","family":"Palmeira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/UCIBIO-i4HB, Laboratory of Microbiology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9383-3663","authenticated-orcid":false,"given":"Maria Ant\u00f3nia","family":"Nunes","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5053-513X","authenticated-orcid":false,"given":"Rita C.","family":"Alves","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8465-6346","authenticated-orcid":false,"given":"Helena","family":"Ferreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/UCIBIO-i4HB, Laboratory of Microbiology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-6596","authenticated-orcid":false,"given":"Maria Beatriz P. P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.scitotenv.2018.06.350","article-title":"Olive pomace as a valuable source of bioactive compounds: A study regarding its lipid- and water-soluble components","volume":"644","author":"Nunes","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_2","unstructured":"Nations, U. (2023, February 07). Transforming Our World: The 2030 Agenda for Sustainable Development. Available online: https:\/\/sdgs.un.org\/sites\/default\/files\/publications\/21252030%20Agenda%20for%20Sustainable%20Development%20web.pdf."},{"key":"ref_3","unstructured":"Commission, E. (2023, February 07). Olive Oil: Detailed Information on the Market Situation, Price Developments, Balance Sheets, Production and Trade. Available online: https:\/\/ec.europa.eu\/info\/food-farming-fisheries\/plants-and-plant-products\/plant-products\/olive-oil_en."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ifset.2016.04.016","article-title":"Olive by-products for functional and food applications: Challenging opportunities to face environmental constraints","volume":"35","author":"Nunes","year":"2016","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.wasman.2019.12.050","article-title":"Fourier transform near infrared spectroscopy as a tool to discriminate olive wastes: The case of monocultivar pomaces","volume":"103","author":"Nunes","year":"2020","journal-title":"Waste Manag."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.jfoodeng.2013.06.027","article-title":"Influence of air temperature on drying kinetics and antioxidant potential of olive pomace","volume":"119","author":"Micol","year":"2013","journal-title":"J. Food Eng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1007\/s11947-012-0802-0","article-title":"Quality Characterization of Waste Olive Cake During Hot Air Drying: Nutritional Aspects and Antioxidant Activity","volume":"6","author":"Uribe","year":"2013","journal-title":"Food Bioprocess Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.foodchem.2013.09.121","article-title":"Influence of process temperature on drying kinetics, physicochemical properties and antioxidant capacity of the olive-waste cake","volume":"147","author":"Uribe","year":"2014","journal-title":"Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1007\/s12649-017-0187-4","article-title":"Influence of Drying on the Recoverable High-Value Products from Olive (cv. Arbequina) Waste Cake","volume":"10","author":"Pasten","year":"2019","journal-title":"Waste Biomass Valorization"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/jsfa.10478","article-title":"Functional compounds from olive pomace to obtain high-added value foods\u2014A review","volume":"101","author":"Difonzo","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ruan, D., Wang, H., Cheng, F., Ruan, D., Wang, H., and Cheng, F. (2018). The Maillard Reaction, Springer.","DOI":"10.1007\/978-3-030-04777-1_1"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Cilla, A., Barber\u00e1, R., L\u00f3pez-Garc\u00eda, G., Blanco-Morales, V., Alegr\u00eda, A., and Garcia-Llatas, G. (2019). Innovative Thermal and Non-Thermal Processing, Bioaccessibility and Bioavailability of Nutrients and Bioactive Compounds, Elsevier.","DOI":"10.1016\/B978-0-12-814174-8.00007-X"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.scitotenv.2018.10.204","article-title":"Valorization of olive pomace by a green integrated approach applying sustainable extraction and membrane-assisted concentration","volume":"652","author":"Nunes","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s13197-011-0365-5","article-title":"Dietary fibre in foods: A review","volume":"49","author":"Dhingra","year":"2012","journal-title":"J. Food Sci. Technol."},{"key":"ref_15","first-page":"127535","article-title":"Polyphenols and their applications: An approach in food chemistry and innovation potential","volume":"338","author":"Pastore","year":"2020","journal-title":"Food Chem."},{"key":"ref_16","first-page":"e157","article-title":"The role of vitamin E in human health and some diseases","volume":"14","author":"Rizvi","year":"2014","journal-title":"Sultan Qaboos Univ. Med. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"109326","DOI":"10.1016\/j.foodres.2020.109326","article-title":"Microencapsulation of vitamins in food applications to prevent losses in processing and storage: A review","volume":"137","author":"Dhakal","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Shahidi, F., and Camargo, A.C.D. (2016). Tocopherols and Tocotrienols in Common and Emerging Dietary Sources: Occurrence, Applications, and Health Benefits. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17101745"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"360438","DOI":"10.1155\/2014\/360438","article-title":"Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal","volume":"2014","author":"Ayala","year":"2014","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1007\/s11130-012-0315-z","article-title":"Comprehensive Analysis of Polyphenols in 55 Extra Virgin Olive Oils by HPLC-ECD and Their Correlation with Antioxidant Activities","volume":"67","author":"Bayram","year":"2012","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"100802","DOI":"10.1016\/j.fbio.2020.100802","article-title":"Thermal treatment of apple puree under oxygen-free condition: Effect on phenolic compounds, ascorbic acid, antioxidant activities, color, and enzyme activities","volume":"39","author":"Kim","year":"2021","journal-title":"Food Biosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.foodchem.2016.08.102","article-title":"Antioxidant properties of selected fruit cultivars grown in Sri Lanka","volume":"238","author":"Silva","year":"2018","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., and Rupasinghe, H.P.V. (2018). Effect of Different Cooking Methods on Polyphenols, Carotenoids and Antioxidant Activities of Selected Edible Leaves. Antioxidants, 7.","DOI":"10.3390\/antiox7090117"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.lwt.2012.03.029","article-title":"The fate of olive fruit phenols during commercial olive oil processing: Traditional press versus continuous two- and three-phase centrifuge","volume":"49","author":"Klen","year":"2012","journal-title":"LWT Food Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Leonardis, A.D., Macciola, V., and Iacovino, S. (2020). Delivery Systems for Hydroxytyrosol Supplementation: State of the Art. Colloids Interfaces, 4.","DOI":"10.3390\/colloids4020025"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.foodchem.2016.03.011","article-title":"Hydroxytyrosol in functional hydroxytyrosol-enriched biscuits is highly bioavailable and decreases oxidised low density lipoprotein levels in humans","volume":"205","author":"Mateos","year":"2016","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.foodchem.2017.03.106","article-title":"Nutritional, chemical and antioxidant\/pro-oxidant profiles of silverskin, a coffee roasting by-product","volume":"267","author":"Costa","year":"2018","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"109683","DOI":"10.1016\/j.foodres.2020.109683","article-title":"Valorization of by-products from olive oil industry and added-value applications for innovative functional foods","volume":"137","author":"Astray","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_29","unstructured":"Ratnayaka, D.D., Brandt, M.J., and Johnson, K.M. (2009). Water Supply, Butterworth-Heinemann. [6th ed.]."},{"key":"ref_30","unstructured":"Lelieveld, Y.M.H. (2014). Food Safety Management, Academic Press."},{"key":"ref_31","unstructured":"Berk, Z. (2018). Food Process Engineering and Technology, Academic Press. [3rd ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Fellows, P.J. (2009). Food Processing Technology: Principles and Practice, Elsevier.","DOI":"10.1533\/9781845696344"},{"key":"ref_33","unstructured":"AOAC (2019). Official Methods of Analysis, Association of Official Analytical Chemists. [21st ed.]."},{"key":"ref_34","unstructured":"Tontisirin, K. (2003). Chapter 2: Methods of Food Analysis. Food Energy: Methods of Analysis and Conversion Factors: Report of a Technical Workshop, Food and Agriculture Organization of the United Nations."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1177\/1082013208102695","article-title":"Determination of vitamin E in coffee beans by HPLC using a micro-extraction method","volume":"15","author":"Alves","year":"2009","journal-title":"Food Sci. Technol. Int."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ferreira, D.M., Nunes, M.A., Santo, L.E., Machado, S., Costa, A.S.G., \u00c1lvarez-Ort\u00ed, M., Pardo, J.E., Oliveira, M.B.P.P., and Alves, R.C. (2023). Characterization of Chia Seeds, Cold-Pressed Oil, and Defatted Cake: An Ancient Grain for Modern Food Production. Molecules, 28.","DOI":"10.3390\/molecules28020723"},{"key":"ref_37","unstructured":"(2017). Animal and Vegetable Fats and Oils\u2014Gas Chromatography of Fatty Acid Methyl Esters: Part 2: Preparation of Methyl Esters of Fatty Acids (Standard No. ISO 12966)."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2876\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:00:57Z","timestamp":1760122857000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,22]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["molecules28062876"],"URL":"https:\/\/doi.org\/10.3390\/molecules28062876","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,22]]}}}