{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T07:54:20Z","timestamp":1768895660764,"version":"3.49.0"},"reference-count":78,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T00:00:00Z","timestamp":1681257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF) under the scope of NORTE 2020, part of the PORTUGAL 2020 Partnership Agreement, through the project ATLANTIDA","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}]},{"name":"European Regional Development Fund (ERDF) under the scope of NORTE 2020, part of the PORTUGAL 2020 Partnership Agreement, through the project ATLANTIDA","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}]},{"name":"European Regional Development Fund (ERDF) under the scope of NORTE 2020, part of the PORTUGAL 2020 Partnership Agreement, through the project ATLANTIDA","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}]},{"name":"European Regional Development Fund (ERDF) under the scope of NORTE 2020, part of the PORTUGAL 2020 Partnership Agreement, through the project ATLANTIDA","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}]},{"name":"European Regional Development Fund (ERDF) under the scope of NORTE 2020, part of the PORTUGAL 2020 Partnership Agreement, through the project ATLANTIDA","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}]},{"name":"R&amp;D Infrastructure TERM RES-Hub PINFRA\/22190\/2016","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}]},{"name":"R&amp;D Infrastructure TERM RES-Hub PINFRA\/22190\/2016","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}]},{"name":"R&amp;D Infrastructure TERM RES-Hub PINFRA\/22190\/2016","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}]},{"name":"R&amp;D Infrastructure TERM RES-Hub PINFRA\/22190\/2016","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}]},{"name":"R&amp;D Infrastructure TERM RES-Hub PINFRA\/22190\/2016","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}]},{"DOI":"10.13039\/501100001871","name":"PhD scholarship of A.L.A.","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"PhD scholarship of A.L.A.","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"PhD scholarship of A.L.A.","doi-asserted-by":"publisher","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"PhD scholarship of A.L.A.","doi-asserted-by":"publisher","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"PhD scholarship of A.L.A.","doi-asserted-by":"publisher","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Doctoral Programme Do*Mar and the PhD scholarship of R.O.S.","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Doctoral Programme Do*Mar and the PhD scholarship of R.O.S.","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Doctoral Programme Do*Mar and the PhD scholarship of R.O.S.","doi-asserted-by":"publisher","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Doctoral Programme Do*Mar and the PhD scholarship of R.O.S.","doi-asserted-by":"publisher","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Doctoral Programme Do*Mar and the PhD scholarship of R.O.S.","doi-asserted-by":"publisher","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Doctoral Programme in Advanced Therapies for Health, PATH","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}]},{"name":"Doctoral Programme in Advanced Therapies for Health, PATH","award":["Norte-01-0145-FEDER-022190"],"award-info":[{"award-number":["Norte-01-0145-FEDER-022190"]}]},{"name":"Doctoral Programme in Advanced Therapies for Health, PATH","award":["CEECIND\/04687\/2017"],"award-info":[{"award-number":["CEECIND\/04687\/2017"]}]},{"name":"Doctoral Programme in Advanced Therapies for Health, PATH","award":["PD\/BD\/127995\/2016"],"award-info":[{"award-number":["PD\/BD\/127995\/2016"]}]},{"name":"Doctoral Programme in Advanced Therapies for Health, PATH","award":["PD\/BD\/150519\/2019"],"award-info":[{"award-number":["PD\/BD\/150519\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Collagen is the major structural protein in extracellular matrix present in connective tissues, including skin, being considered a promising material for skin regeneration. Marine organisms have been attracting interest amongst the industry as an alternative collagen source. In the present work, Atlantic codfish skin collagen was analyzed, to evaluate its potential for skincare. The collagen was extracted from two different skin batches (food industry by-product) using acetic acid (ASColl), confirming the method reproducibility since no significant yield differences were observed. The extracts characterization confirmed a profile compatible with type I collagen, without significant differences between batches or with bovine skin collagen (a reference material in biomedicine). Thermal analyses suggested ASColl\u2019s native structure loss at 25 \u00b0C, and an inferior thermal stability to bovine skin collagen. No cytotoxicity was found for ASColl up to 10 mg\/mL in keratinocytes (HaCaT cells). ASColl was used to develop membranes, which revealed smooth surfaces without significative morphological or biodegradability differences between batches. Their water absorption capacity and water contact angle indicated a hydrophilic feature. The metabolic activity and proliferation of HaCaT were improved by the membranes. Hence, ASColl membranes exhibited attractive characteristics to be applied in the biomedical and cosmeceutical field envisaging skincare.<\/jats:p>","DOI":"10.3390\/molecules28083394","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T02:09:21Z","timestamp":1681351761000},"page":"3394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Potential of Atlantic Codfish (Gadus morhua) Skin Collagen for Skincare Biomaterials"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5937-2253","authenticated-orcid":false,"given":"Cristina V.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3943-9996","authenticated-orcid":false,"given":"Rita O.","family":"Sousa","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-5372","authenticated-orcid":false,"given":"Ana C.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1552-4056","authenticated-orcid":false,"given":"Ana L.","family":"Alves","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3650-1830","authenticated-orcid":false,"given":"Catarina F.","family":"Marques","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2430-7758","authenticated-orcid":false,"given":"Mariana T.","family":"Cerqueira","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4295-6129","authenticated-orcid":false,"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8520-603X","authenticated-orcid":false,"given":"Tiago H.","family":"Silva","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark\u2014Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4806-909 Braga\/Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.ejphar.2017.08.003","article-title":"In vivo efficiency of the collagen coated nanofibrous scaffold and their effect on growth factors and pro-inflammatory cytokines in wound healing","volume":"814","author":"Ramanathan","year":"2017","journal-title":"Eur. J. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Alves, A.L., Marques, A.L.P., Martins, E., Silva, T.H., and Reis, R.L. (2017). Cosmetic Potential of Marine Fish Skin Collagen. Cosmetics, 4.","DOI":"10.3390\/cosmetics4040039"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ijbiomac.2015.02.050","article-title":"Marine-derived biological macromolecule-based biomaterials for wound healing and skin tissue regeneration","volume":"77","author":"Chandika","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Carvalho, A.M., Marques, A.P., Silva, T.H., and Reis, R.L. (2018). Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications. Mar. Drugs, 16.","DOI":"10.3390\/md16120495"},{"key":"ref_5","first-page":"549","article-title":"The role of extracellular matrix components in pin bone attachments during storage\u2014A comparison between farmed Atlantic salmon (Salmo salar) and cod (Gadus morhua L.)","volume":"43","author":"Krasnov","year":"2016","journal-title":"Fish Physiol. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"98","DOI":"10.5582\/irdr.2019.01064","article-title":"Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen","volume":"8","author":"Lu","year":"2019","journal-title":"Intractable Rare Dis. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Arseni, L., Lombardi, A., and Orioli, D. (2018). From Structure to Phenotype: Impact of Collagen Alterations on Human Health. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051407"},{"key":"ref_8","unstructured":"Madhukar, S. (2015). Research Trends in Biochemistry, Molecular Biology and Microbiology, SM Group."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rajendran, S. (2009). Advanced Textiles for Wound Care, Woodhead Publishing.","DOI":"10.1533\/9781845696306"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1146\/annurev-food-031414-111800","article-title":"Collagen and Gelatin","volume":"6","author":"Liu","year":"2015","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lim, Y.-S., Ok, Y.-J., Hwang, S.-Y., Kwak, J.-Y., and Yoon, S. (2019). Marine Collagen as a Promising Biomaterial for Biomedical Applications. Mar. Drugs, 17.","DOI":"10.3390\/md17080467"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5881","DOI":"10.3390\/md12125881","article-title":"Marine Origin Collagens and Its Potential Applications","volume":"12","author":"Silva","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Venkatesan, J., Anil, S., Kim, S.-K., and Shim, M.S. (2017). Marine Fish Proteins and Peptides for Cosmeceuticals: A Review. Mar. Drugs, 15.","DOI":"10.3390\/md15050143"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1515\/rams-2018-0002","article-title":"Collagen as a Potential Biomaterial in Biomedical Applications","volume":"53","author":"Muthukumar","year":"2018","journal-title":"Rev. Adv. Mater. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1007\/s13197-011-0440-y","article-title":"Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization","volume":"50","author":"Muralidharan","year":"2011","journal-title":"J. Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Moreira-Silva, J., Diogo, G.S., Marques, A.L.P., Silva, T.H., and Reis, R.L. (2016). Marine Collagen Isolation and Processing Envisaging Biomedical Applications, John Wiley & Sons.","DOI":"10.1002\/9781119126218.ch2"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"100315","DOI":"10.1016\/j.scp.2020.100315","article-title":"Collagen extraction and recent biological activities of collagen peptides derived from sea-food waste: A review","volume":"18","author":"Ahmed","year":"2020","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"110852","DOI":"10.1016\/j.colsurfb.2020.110852","article-title":"Optimization of the collagen extraction from Nile tilapia skin (Oreochromis niloticus) and its hydrogel with hyaluronic acid","volume":"189","author":"Menezes","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"127489","DOI":"10.1016\/j.foodchem.2020.127489","article-title":"Physicochemical and functional properties of Maillard reaction products derived from cod (Gadus morhua L.) skin collagen peptides and xylose","volume":"333","author":"Chen","year":"2020","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sanchez, A., Blanco, M., Correa, B., Perez-Martin, R.I., and Sotelo, C.G. (2018). Effect of Fish Collagen Hydrolysates on Type I Collagen mRNA Levels of Human Dermal Fibroblast Culture. Mar. Drugs, 16.","DOI":"10.3390\/md16050144"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1002\/term.3005","article-title":"Wound healing grafts: Omega-3 fatty acid lipid content differentiates the lipid profiles of acellular Atlantic cod skin from traditional dermal substitutes","volume":"14","author":"Kotronoulas","year":"2019","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/978-981-13-0947-2_21","article-title":"Collagen Type I: A Versatile Biomaterial","volume":"1077","author":"Chowdhury","year":"2018","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ge, B., Wang, H., Li, J., Liu, H., Yin, Y., Zhang, N., and Qin, S. (2020). Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings. Mar. Drugs, 18.","DOI":"10.3390\/md18040178"},{"key":"ref_24","first-page":"20","article-title":"Collagen: A review on its sources and potential cosmetic applications","volume":"17","author":"Barroso","year":"2017","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s10965-020-02048-x","article-title":"Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare","volume":"27","author":"Sousa","year":"2020","journal-title":"J. Polym. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e1700557","DOI":"10.1002\/cbdv.201700557","article-title":"Collagen from Marine Biological Sources and Medical Applications","volume":"15","author":"Felician","year":"2018","journal-title":"Chem. Biodivers."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.joca.2012.04.008","article-title":"Symptomatic and chondroprotective treatment with collagen derivatives in osteoarthritis: A systematic review","volume":"20","author":"Luijsterburg","year":"2012","journal-title":"Osteoarthr. Cartil."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Fassini, D., Oliveira, S.M., Silva, T.H., and Reis, R.L. (2020). Biotechnological Valorization of Marine Collagens, John Wiley & Sons.","DOI":"10.1002\/9781119143802.ch35"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1002\/mame.201500028","article-title":"Biodegradable Polymeric Films and Membranes Processing and Forming for Tissue Engineering","volume":"300","author":"Suntornnond","year":"2015","journal-title":"Macromol. Mater. Eng."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1177\/0885328210394470","article-title":"Heterofunctional nanosheet controlling cell adhesion properties by collagen coating","volume":"27","author":"Niwa","year":"2011","journal-title":"J. Biomater. Appl."},{"key":"ref_31","unstructured":"\u00c5gren, M.S. (2016). Wound Healing Biomaterials, Woodhead Publishing."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2892","DOI":"10.1002\/jps.21210","article-title":"Wound Healing Dressings and Drug Delivery Systems: A Review","volume":"97","author":"Boateng","year":"2008","journal-title":"J. Pharm. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1002\/bip.22486","article-title":"Collagen-based biomaterials for wound healing","volume":"101","author":"Chattopadhyay","year":"2014","journal-title":"Biopolymers"},{"key":"ref_34","first-page":"135","article-title":"Collagen-based formulations for wound healing applications","volume":"2","author":"Lee","year":"2016","journal-title":"Wound Health Biomater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e1801651","DOI":"10.1002\/adma.201801651","article-title":"The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development","volume":"31","author":"Sorushanova","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_36","unstructured":"Pal, K., Banerjee, I., Sarkar, P., Kim, D., Deng, W.-P., Dubey, N.K., and Majumder, K. (2020). Biopolymer-Based Formulations, Elsevier."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.1021\/la903073v","article-title":"Nanomechanical Properties of Thin Films of Type I Collagen Fibrils","volume":"26","author":"Chung","year":"2010","journal-title":"Langmuir"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1080\/09205063.2019.1669313","article-title":"Acid and enzymatic extraction of collagen from Atlantic cod (Gadus morhua) swim bladders envisaging health-related applications","volume":"31","author":"Sousa","year":"2019","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/jsfa.7154","article-title":"Comparative study on acid-soluble and pepsin-soluble collagens from skin and swim bladder of grass carp (Ctenopharyngodon idella)","volume":"96","author":"Wu","year":"2015","journal-title":"J. Sci. Food Agric."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8940","DOI":"10.1039\/D1GC01432C","article-title":"Uncovering the potential of aqueous solutions of deep eutectic solvents on the extraction and purification of collagen type I from Atlantic codfish (Gadus morhua)","volume":"23","author":"Bisht","year":"2021","journal-title":"Green Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1016\/j.foodchem.2007.04.030","article-title":"The influence of different acids and pepsin on the extractability of collagen from the skin of Baltic cod (Gadus morhua)","volume":"105","author":"Skierka","year":"2007","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jafari, H., Lista, A., Siekapen, M.M., Ghaffari-Bohlouli, P., Nie, L., Alimoradi, H., and Shavandi, A. (2020). Fish Collagen: Extraction, Characterization, and Applications for Biomaterials Engineering. Polymers, 12.","DOI":"10.3390\/polym12102230"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.ijbiomac.2017.08.044","article-title":"A comparative study of the properties and self-aggregation behavior of collagens from the scales and skin of grass carp (Ctenopharyngodon idella)","volume":"106","author":"Liu","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Simard, M., Grenier, A., Rioux, G., Tremblay, A., Blais, I., Flamand, N., and Pouliot, R. (2022). Remodeling of the Dermal Extracellular Matrix in a Tissue-Engineered Psoriatic Skin Model by n-3 Polyunsaturated Fatty Acids. Biomedicines, 10.","DOI":"10.3390\/biomedicines10051078"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15572","DOI":"10.1002\/jcb.28824","article-title":"Biodegradable collagen from Scomberomorus lineolatus skin for wound healing dressings and its application on antibiofilm properties","volume":"120","author":"Abinaya","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10989-012-9291-2","article-title":"Wound Healing Properties of Collagen from the Bone of Two Marine Fishes","volume":"18","author":"Kumar","year":"2012","journal-title":"Int. J. Pept. Res. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"126775","DOI":"10.1016\/j.foodchem.2020.126775","article-title":"Correlation of proline, hydroxyproline and serine content, denaturation temperature and circular dichroism analysis of type I collagen with the physiological temperature of marine teleosts","volume":"329","author":"Akita","year":"2020","journal-title":"Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"112238","DOI":"10.1016\/j.colsurfb.2021.112238","article-title":"Typical structure, biocompatibility, and cell proliferation bioactivity of collagen from Tilapia and Pacific cod","volume":"210","author":"Song","year":"2021","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_49","first-page":"818","article-title":"Preparation and Characterisation of Collagen from Freshwater Fish Scales","volume":"02","author":"Zhang","year":"2011","journal-title":"Food Nutr. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.bbapap.2005.06.005","article-title":"How to study proteins by circular dichroism","volume":"1751","author":"Kelly","year":"2005","journal-title":"Biochim. Biophys. Acta Proteins Proteom."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1038\/nprot.2006.204","article-title":"Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions","volume":"1","author":"Greenfield","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.foodchem.2004.02.006","article-title":"Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus)","volume":"88","author":"Ogawa","year":"2004","journal-title":"Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1002\/jsfa.5854","article-title":"Isolation and characterization of pepsin-solubilized collagen from the integument of sea cucumber (Stichopus vastus)","volume":"93","author":"Abedin","year":"2012","journal-title":"J. Sci. Food Agric."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"302932","DOI":"10.1155\/2014\/302932","article-title":"Potency of Fish Collagen as a Scaffold for Regenerative Medicine","volume":"2014","author":"Yamada","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.fbio.2016.12.002","article-title":"Effect of chemical and biological cross-linkers on mechanical and functional properties of shark catfish skin collagen films","volume":"17","author":"Elango","year":"2017","journal-title":"Food Biosci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"E450","DOI":"10.1111\/j.1750-3841.2007.00478.x","article-title":"Isolation and Characterization of Collagen from the Skin of Deep-Sea Redfish (Sebastes mentella)","volume":"72","author":"Wang","year":"2007","journal-title":"J. Food Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.ijbiomac.2015.07.032","article-title":"Isolation and characterization of collagen from the skin of Brama australis","volume":"80","author":"Sionkowska","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/j.foodchem.2008.06.020","article-title":"Properties of collagen from skin, scale and bone of carp (Cyprinus carpio)","volume":"112","author":"Duan","year":"2009","journal-title":"Food Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.procbio.2018.07.009","article-title":"Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering","volume":"74","author":"Li","year":"2018","journal-title":"Process Biochem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104711","DOI":"10.1016\/j.supflu.2019.104711","article-title":"Supercritical impregnation of mesoglycan into calcium alginate aerogel for wound healing","volume":"157","author":"Franco","year":"2019","journal-title":"J. Supercrit. Fluids"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Rajendran, S. (2019). Advanced Textiles for Wound Care, Woodhead Publishing. [2nd ed.].","DOI":"10.1016\/B978-0-08-102192-7.00006-0"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Hayashi, Y., Yamada, S., Ikeda, T., and Yanagiguchi, K. (2011). Fish Collagen and Tissue Repair, CRC Press.","DOI":"10.1201\/b10120-12"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1016\/j.biomaterials.2006.01.015","article-title":"Collagen scaffolds derived from a marine source and their biocompatibility","volume":"27","author":"Song","year":"2006","journal-title":"Biomaterials"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"667","DOI":"10.3390\/jfb6030667","article-title":"Compositional and in Vitro Evaluation of Nonwoven Type I Collagen\/Poly-dl-lactic Acid Scaffolds for Bone Regeneration","volume":"6","author":"Qiao","year":"2015","journal-title":"J. Funct. Biomater."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.msec.2016.05.021","article-title":"Evaluation of epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes and concerns on osteoblasts","volume":"67","author":"Chu","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_66","unstructured":"Grumezescu, A.M. (2017). Nano- and Microscale Drug Delivery Systems, Elsevier."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.ijpharm.2017.07.027","article-title":"Evaluation of three-layered doxycycline-collagen loaded nanofiber wound dressing","volume":"529","author":"Tort","year":"2017","journal-title":"Int. J. Pharm."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"103","DOI":"10.2165\/00063030-200418020-00004","article-title":"The Application of Recombinant Human Collagen in Tissue Engineering","volume":"18","author":"Yang","year":"2004","journal-title":"Biodrugs"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"767","DOI":"10.2298\/VSP160117116B","article-title":"The use of collagen membranes in guided tissue regeneration","volume":"74","author":"Bubalo","year":"2017","journal-title":"Vojn. Pregl."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"172","DOI":"10.3349\/ymj.2001.42.2.172","article-title":"Characterization of UV-irradiated dense\/porous collagen membranes: Morphology, enzymatic degradation, and mechanical properties","volume":"42","author":"Lee","year":"2001","journal-title":"Yonsei Med. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1002\/(SICI)1097-4636(19980605)40:3<358::AID-JBM3>3.0.CO;2-E","article-title":"A study on the grafting reaction of isocyanates with hydroxyapatite particles","volume":"40","author":"Liu","year":"1998","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Diogo, G.S., L\u00f3pez-Senra, E.L., Pirraco, R.P., Canadas, R.F., Fernandes, E.M., Serra, J., P\u00e9rez-Mart\u00edn, R.I., Sotelo, C.G., Marques, A.P., and Gonz\u00e1lez, P. (2018). Marine Collagen\/Apatite Composite Scaffolds Envisaging Hard Tissue Applications. Mar. Drugs, 16.","DOI":"10.3390\/md16080269"},{"key":"ref_73","unstructured":"International Organization for Standardization (2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity, International Organization for Standardization."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.colsurfb.2016.03.052","article-title":"Electrospun tilapia collagen nanofibers accelerating wound healing via inducing keratinocytes proliferation and differentiation","volume":"143","author":"Zhou","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.msec.2018.08.026","article-title":"Accelerated wound healing and its promoting effects of biomimetic collagen matrices with siderophore loaded gelatin microspheres in tissue engineering","volume":"93","author":"Ramanathan","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.3390\/molecules15042845","article-title":"Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment","volume":"15","author":"Asadinezhad","year":"2010","journal-title":"Molecules"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"105059","DOI":"10.1016\/j.reactfunctpolym.2021.105059","article-title":"Review of advances in polymeric wound dressing films","volume":"168","author":"Savencu","year":"2021","journal-title":"React. Funct. Polym."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"113220","DOI":"10.1016\/j.jep.2020.113220","article-title":"In vitro antibacterial activity of extracts from Samoan medicinal plants and their effect on proliferation and migration of human fibroblasts","volume":"264","author":"Frankova","year":"2020","journal-title":"J. Ethnopharmacol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/8\/3394\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:14:34Z","timestamp":1760123674000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/8\/3394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,12]]},"references-count":78,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["molecules28083394"],"URL":"https:\/\/doi.org\/10.3390\/molecules28083394","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,12]]}}}