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(OE)","award":["PTDC\/BAA-AGR\/30936\/2017"],"award-info":[{"award-number":["PTDC\/BAA-AGR\/30936\/2017"]}]},{"name":"Portuguese national funds (OE)","award":["46080"],"award-info":[{"award-number":["46080"]}]},{"name":"Portuguese national funds (OE)","award":["POCI-01-0247-FEDER-046080"],"award-info":[{"award-number":["POCI-01-0247-FEDER-046080"]}]},{"name":"Portuguese national funds (OE)","award":["LISBOA-01-0247-FEDER-046080"],"award-info":[{"award-number":["LISBOA-01-0247-FEDER-046080"]}]},{"name":"Portuguese national funds (OE)","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"Portuguese national funds (OE)","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}]},{"name":"Portuguese national funds (OE)","award":["UIDB\/50011\/2020"],"award-info":[{"award-number":["UIDB\/50011\/2020"]}]},{"name":"Portuguese national funds (OE)","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}]},{"name":"Portuguese national funds (OE)","award":["CDL-CTTRI-88-ARH\/2018\u2014REF. 049-88-ARH\/2018"],"award-info":[{"award-number":["CDL-CTTRI-88-ARH\/2018\u2014REF. 049-88-ARH\/2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Brewer\u2019s spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties. The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure\/function relationships. This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources. To achieve this, structure\/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties. Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1\u21924)- and (\u03b21\u21923)-linked glucans, 33 and 12%, respectively. Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and \u03b2-glucans yielded the best emulsions by using ultraturrax stirring. \u03b2-Glucans and O-linked mannoproteins were found to contribute to emulsion stability by preventing Ostwald ripening. When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers. When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1\/3 of their concentration. This shows that BSY alkali soluble mannoproteins and subcritical water extracted \u03b2-glucans can be used as replacers of animal protein and additives in sauces.<\/jats:p>","DOI":"10.3390\/molecules28083540","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T01:36:45Z","timestamp":1681781805000},"page":"3540","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Brewer\u2019s Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6817-0776","authenticated-orcid":false,"given":"Sofia F.","family":"Reis","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4647-5274","authenticated-orcid":false,"given":"Pedro A. R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4887-4167","authenticated-orcid":false,"given":"V\u00edtor J.","family":"Martins","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Sara","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Lu\u00eds P.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0372-2493","authenticated-orcid":false,"given":"V\u00edtor M.","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Diogo","family":"Figueira","sequence":"additional","affiliation":[{"name":"Mendes Gon\u00e7alves SA, Zona Industrial, Lote 6, 2154-909 Goleg\u00e3, Portugal"}]},{"given":"Diogo","family":"Castelo-Branco","sequence":"additional","affiliation":[{"name":"Mendes Gon\u00e7alves SA, Zona Industrial, Lote 6, 2154-909 Goleg\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-3765","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8898-6342","authenticated-orcid":false,"given":"Manuel A.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2083-9634","authenticated-orcid":false,"given":"Elisabete","family":"Coelho","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"McClements, D.J. (2015). Food Emulsions, CRC Press.","DOI":"10.1201\/b18868"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jfoodeng.2014.06.015","article-title":"Formation and Stabilization of Nanoemulsion-Based Vitamin E Delivery Systems Using Natural Surfactants: Quillaja Saponin and Lecithin","volume":"142","author":"Ozturk","year":"2014","journal-title":"J. Food Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev-food-030216-030154","article-title":"Recent Advances in the Utilization of Natural Emulsifiers to Form and Stabilize Emulsions","volume":"8","author":"McClements","year":"2017","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.foodhyd.2014.10.024","article-title":"Influence of Xanthan Gum on Oil-in-Water Emulsion Characteristics Stabilized by OSA Starch","volume":"45","year":"2015","journal-title":"Food Hydrocoll."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"109376","DOI":"10.1016\/j.foodres.2020.109376","article-title":"Recent Advances in Improving Stability of Food Emulsion by Plant Polysaccharides","volume":"137","author":"Shao","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sridhar, K., Bouhallab, S., Croguennec, T., Renard, D., and Lechevalier, V. (2022). Recent Trends in Design of Healthier Plant-Based Alternatives: Nutritional Profile, Gastrointestinal Digestion, and Consumer Perception. Crit. Rev. Food Sci. Nutr., 1\u201316.","DOI":"10.1080\/10408398.2022.2081666"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s43014-023-00129-0","article-title":"Trends and Innovations in the Formulation of Plant-Based Foods","volume":"5","author":"Tachie","year":"2023","journal-title":"Food Prod. Process. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1128\/aem.54.6.1420-1425.1988","article-title":"The Mannoprotein of Saccharomyces cerevisiae Is an Effective Bioemulsifier","volume":"54","author":"Cameron","year":"1988","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"112273","DOI":"10.1016\/j.lwt.2021.112273","article-title":"Red and White Wine Lees as a Novel Source of Emulsifiers and Foaming Agents","volume":"152","author":"Marangon","year":"2021","journal-title":"LWT"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"124867","DOI":"10.1016\/j.foodchem.2019.05.141","article-title":"Characterization of the Composition and the Techno-Functional Properties of Mannoproteins from Saccharomyces cerevisiae Yeast Cell Walls","volume":"297","author":"Li","year":"2019","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"126264","DOI":"10.1016\/j.foodchem.2020.126264","article-title":"Cell Surface Changes That Advance the Application of Using Yeast as a Food Emulsifier","volume":"315","author":"Nerome","year":"2020","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1534\/genetics.112.144485","article-title":"Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall","volume":"192","author":"Orlean","year":"2012","journal-title":"Genetics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"118826","DOI":"10.1016\/j.carbpol.2021.118826","article-title":"Brewer\u2019s Yeast Polysaccharides\u2014A Review of Their Exquisite Structural Features and Biomedical Applications","volume":"277","author":"Bastos","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.carbpol.2014.03.010","article-title":"Valuation of Brewers Spent Yeast Polysaccharides: A Structural Characterization Approach","volume":"116","author":"Pinto","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109352","DOI":"10.1016\/j.foodres.2020.109352","article-title":"Current and Future Strategies for Wine Yeast Lees Valorization","volume":"137","author":"Lomolino","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1074505","DOI":"10.3389\/frfst.2022.1074505","article-title":"\u03b2-Glucan from Brewer\u2019s Spent Yeast as a Techno-Functional Food Ingredient","volume":"2","author":"Egea","year":"2022","journal-title":"Front. Food Sci. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.carbpol.2015.02.031","article-title":"Modifications of Saccharomyces pastorianus Cell Wall Polysaccharides with Brewing Process","volume":"124","author":"Bastos","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.foodchem.2019.05.006","article-title":"Revisiting the Chemistry of Apple Pomace Polyphenols","volume":"294","author":"Fernandes","year":"2019","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"120325","DOI":"10.1016\/j.carbpol.2022.120325","article-title":"Structural Differences on Cell Wall Polysaccharides of Brewer\u2019s Spent Saccharomyces and Microarray Binding Profiles with Immune Receptors","volume":"301","author":"Reis","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"113284","DOI":"10.1016\/j.lwt.2022.113284","article-title":"Effect of Preparation Methods on Physiochemical and Functional Properties of Yeast \u03b2-Glucan","volume":"160","author":"Fu","year":"2022","journal-title":"LWT"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.ijbiomac.2010.06.013","article-title":"Characterization of an Unexpected Bioemulsifier from Spent Yeast Obtained from Thai Traditional Liquor Distillation","volume":"47","author":"Dikit","year":"2010","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"106321","DOI":"10.1016\/j.foodhyd.2020.106321","article-title":"Identification of Yeast-Derived Emulsification Proteins through Analyses of Proteins Distributed into the Emulsified Phase","volume":"112","author":"Onishi","year":"2021","journal-title":"Food Hydrocoll."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"113898","DOI":"10.1016\/j.lwt.2022.113898","article-title":"Structural Characterization and Emulsifier Property of Yeast Mannoprotein Enzymatically Prepared with a \u03b2-1,6-Glucanase","volume":"168","author":"Qiao","year":"2022","journal-title":"LWT"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1111\/1750-3841.15584","article-title":"Stress Response and Characterization of Oil-in-Water Emulsions Stabilized with Kluyveromyces marxianus Mannoprotein","volume":"86","author":"Hajhosseini","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0268-005X(01)00091-1","article-title":"Hydrocolloids in Emulsions: Particle Size Distribution and Interfacial Activity","volume":"15","author":"Huang","year":"2001","journal-title":"Food Hydrocoll."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1006\/fstl.1996.0114","article-title":"Preparation and Characterisation of Bioemulsifier From Saccharomyces cerevisiae and Its Application in Food Products","volume":"29","author":"Torabizadeh","year":"1996","journal-title":"LWT Food Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.ifset.2013.12.013","article-title":"Followed Extraction of \u03b2-Glucan and Mannoprotein from Spent Brewer\u2019s Yeast (Saccharomyces uvarum) and Application of the Obtained Mannoprotein as a Stabilizer in Mayonnaise","volume":"23","author":"Costa","year":"2014","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.lwt.2014.06.050","article-title":"Stability, Nutritional and Sensory Characteristics of French Salad Dressing Made with Mannoprotein from Spent Brewer\u2019s Yeast","volume":"62","author":"Magnani","year":"2015","journal-title":"LWT Food Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1111\/j.1365-2621.1995.tb06289.x","article-title":"Nitrogen-to-Protein Conversion Factors for Some Common Edible Mushrooms","volume":"60","author":"Fujihara","year":"1995","journal-title":"J. Food Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Linskens, H.F., and Jackson, J.F. (1996). Plant Cell Wall Analysis, Springer.","DOI":"10.1007\/978-3-642-60989-3"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0008-6215(84)85242-8","article-title":"A Simple and Rapid Method for the Permethylation of Carbohydrates","volume":"131","author":"Ciucanu","year":"1984","journal-title":"Carbohydr. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/j.lwt.2006.11.007","article-title":"Rheological, Texture and Sensory Properties of Low-Fat Mayonnaise with Different Fat Mimetics","volume":"40","author":"Liu","year":"2007","journal-title":"LWT Food Sci. Technol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/8\/3540\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:17:39Z","timestamp":1760123859000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/8\/3540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,17]]},"references-count":32,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["molecules28083540"],"URL":"https:\/\/doi.org\/10.3390\/molecules28083540","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,17]]}}}