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Resection, radiation therapy, and temozolomide (TMZ) are insufficient to increase survival, making the treatment limited. Thus, the search for more effective and specific treatments is essential, making plants a promising source for elucidating new anti-glioblastoma compounds. Accordingly, this study investigated the effects of four fractions of hexane and ethyl acetate extract of Annona coriacea Mart., enriched with acetogenins, against GBM cell lines. All four fractions were selectively cytotoxic to GBM cells when compared to TMZ. Moreover, A. coriacea fractions delayed cell migration; reduced cytoplasmic projections, the metalloproteinase 2 (MMP-2) activity; and induced morphological changes characteristic of necroptosis, possibly correlated with the increase in receptor-interacting protein kinase 1 and 3 (RIP-1 and RIP-3), apoptosis-inducing factor (AIF), and the non-activation of cleaved caspase 8. The present findings reinforce that fractions of A. coriacea Mart. should be considered for more studies focusing treatment of GBM.<\/jats:p>","DOI":"10.3390\/molecules28093809","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T12:12:11Z","timestamp":1682943131000},"page":"3809","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acetogenins-Rich Fractions of Annona coriacea Suppress Human Glioblastoma Viability and Migration by Regulating Necroptosis and MMP-2 Activity In Vitro"],"prefix":"10.3390","volume":"28","author":[{"given":"Lorena R.","family":"Sousa","sequence":"first","affiliation":[{"name":"Experimental Pathology Laboratory, Federal University of S\u00e3o Jo\u00e3o del Rei (UFSJ), 400, Sebasti\u00e3o Gon\u00e7alves Coelho, Chanadour, Divin\u00f3polis 35501-296, MG, Brazil"}]},{"given":"Ana Gabriela S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratory, Federal University of S\u00e3o Jo\u00e3o del Rei (UFSJ), 400, Sebasti\u00e3o Gon\u00e7alves Coelho, Chanadour, Divin\u00f3polis 35501-296, MG, Brazil"}]},{"given":"Ant\u00f4nio","family":"Arantes","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratory, Federal University of S\u00e3o Jo\u00e3o del Rei (UFSJ), 400, Sebasti\u00e3o Gon\u00e7alves Coelho, Chanadour, Divin\u00f3polis 35501-296, MG, Brazil"}]},{"given":"Jo\u00e3o Gabriel M.","family":"Junqueira","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Federal University of Goi\u00e1s (UFG), University Campus, Goi\u00e2nia 74968-755, GO, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9418-3706","authenticated-orcid":false,"given":"Gerso P.","family":"Alexandre","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Federal University of Goi\u00e1s (UFG), University Campus, Goi\u00e2nia 74968-755, GO, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5384-6657","authenticated-orcid":false,"given":"Vanessa G. 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Subj."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.3892\/or.2017.5391","article-title":"Modulation of MMP-2 and MMP-9 Secretion by Cytokines, Inducers and Inhibitors in Human Glioblastoma T-98G Cells","volume":"37","author":"Roomi","year":"2017","journal-title":"Oncol. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"109390","DOI":"10.1016\/j.cbi.2021.109390","article-title":"Phytochemicals and Biological Properties of Annona Coriacea Mart. (Annonaceae): A Systematic Review from 1971 to 2020","volume":"336","author":"Rocha","year":"2021","journal-title":"Chem. Biol. Interact."},{"key":"ref_5","first-page":"113","article-title":"Acetogenins from Annonaceae","volume":"101","author":"Liaw","year":"2016","journal-title":"Prog. Chem. Org. Nat. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"21140","DOI":"10.18632\/oncotarget.15502","article-title":"Annonaceous Acetogenins Mediated Up-Regulation of Notch2 Exerts Growth Inhibition in Human Gastric Cancer Cells in Vitro","volume":"8","author":"Li","year":"2017","journal-title":"Oncotarget"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1039\/B500186M","article-title":"Acetogenins from Annonaceae: Recent Progress in Isolation, Synthesis and Mechanisms of Action","volume":"22","author":"Bermejo","year":"2005","journal-title":"Nat. Prod. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"783","DOI":"10.3389\/fphar.2019.00783","article-title":"Selective Acetogenins and Their Potential as Anticancer Agents","volume":"10","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1080\/14786419.2020.1803312","article-title":"Qualitative Analysis of the Acetogenins from Annona coriacea (Annonaceae) Leaves by HPLC-Q-Orbitrap and Their Antibacterial Potential against Oral Pathogens","volume":"36","author":"Terezan","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez, M.T., Dur\u00e1n, A.G., Mej\u00edas, F.J.R., Molinillo, J.M.G., Megias, D., Valdivia, M.M., and Mac\u00edas, F.A. (2020). Bio-Guided Isolation of Acetogenins from Annona cherimola Deciduous Leaves: Production of Nanocarriers to Boost the Bioavailability Properties. Molecules, 25.","DOI":"10.3390\/molecules25204861"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s13596-020-00517-8","article-title":"Assessment of Genotoxic Potential of Annonacin and Annona muricata L. Extracts on Human Breast Cancer (MCF-7) Cells","volume":"21","author":"Naik","year":"2020","journal-title":"Adv. Tradit. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.4103\/pr.pr_19_17","article-title":"Annonacin Exerts Antitumor Activity through Induction of Apoptosis and Extracellular Signal-Regulated Kinase Inhibition","volume":"9","author":"Yap","year":"2017","journal-title":"Pharmacogn. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1038\/s41419-018-0772-x","article-title":"Annonacin Promotes Selective Cancer Cell Death via NKA-Dependent and SERCA-Dependent Pathways","volume":"9","author":"Yiallouris","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1139\/v94-044","article-title":"Parvifloracin and Parviflorin: Cytotoxic Bistetrahydrofuran Acetogenins with 35 Carbons from Asimina parviflora (Annonaceae)","volume":"72","author":"Ratnayake","year":"2011","journal-title":"Can. J. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1021\/np50123a015","article-title":"Additional Bioactive Acetogenins, Annomutacin and (2,4-Trans and Cis)-10R-Annonacin-A-Ones, from the Leaves of Annona muricata","volume":"58","author":"Wu","year":"1995","journal-title":"J. Nat. Prod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/0031-9422(93)85024-L","article-title":"Biologically Active Acetogenins from Stem Bark of Asimina Triloba","volume":"33","author":"Zhao","year":"1993","journal-title":"Phytochemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1055\/s-2004-832622","article-title":"Nine New Cytotoxic Monotetrahydrofuranic Annonaceous Acetogenins from Annona montana","volume":"70","author":"Liaw","year":"2004","journal-title":"Planta Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1021\/np960037q","article-title":"Five Novel Mono-Tetrahydrofuran Ring Acetogenins from the Seeds of Annona muricata","volume":"59","author":"Rieser","year":"1996","journal-title":"J. Nat. Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1021\/np970563x","article-title":"Glacins A and B: Two Novel Bioactive Mono-Tetrahydrofuran Acetogenins from Annona glabra","volume":"61","author":"Liu","year":"1998","journal-title":"J. Nat. Prod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S0031-9422(98)00632-3","article-title":"Asitrilobins A and B: Cytotoxic Mono-THF Annonaceous Acetogenins from the Seeds of Asimina Triloba","volume":"50","author":"Woo","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1021\/np000180q","article-title":"Asitrocin, (2,4)-Cis- and Trans-Asitrocinones:\u2009 Novel Bioactive Mono-Tetrahydrofuran Acetogenins from Asimina Triloba Seeds","volume":"63","author":"Kim","year":"2000","journal-title":"J. Nat. Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.phymed.2010.10.010","article-title":"Cytotoxic Activity of Acetogenins and Styryl Lactones Isolated from Goniothalamus undulatus Ridl. Root Extracts against a Lung Cancer Cell Line (COR-L23)","volume":"18","author":"Tantithanaporn","year":"2011","journal-title":"Phytomedicine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1021\/np050422s","article-title":"A Pyrimidine-\u03b2-Carboline and Other Alkaloids from Annona foetida with Antileishmanial Activity","volume":"69","author":"Costa","year":"2006","journal-title":"J. Nat. Prod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1002\/hlca.201400034","article-title":"New Acetogenins from the Seeds of Annona coriacea","volume":"97","author":"Alves","year":"2014","journal-title":"Helv. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1021\/np50040a023","article-title":"Desacetyluvaricin from Uvaria accuminata, Configuration of Uvaricin at C-36","volume":"48","author":"Jolad","year":"1985","journal-title":"J. Nat. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"138","DOI":"10.2174\/1871520615666150629101827","article-title":"Acetogenins as Potential Anticancer Agents","volume":"16","author":"Mangal","year":"2015","journal-title":"Anticancer Agents Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jep.2003.09.014","article-title":"In Vitro Cytotoxic Activity of Thai Medicinal Plants Used Traditionally to Treat Cancer","volume":"90","author":"Itharat","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1038\/nm.3394","article-title":"Microenvironmental Regulation of Tumor Progression and Metastasis","volume":"19","author":"Quail","year":"2013","journal-title":"Nat. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"32","DOI":"10.5530\/pj.2012.32.7","article-title":"Acetogenins from Annona muricata","volume":"4","author":"Ragasa","year":"2012","journal-title":"Pharmacogn. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5571","DOI":"10.1016\/00404-0399(50)1063N-","article-title":"New Calcium-Selective Electrodes Based on Annonaceous Acetogenins and Their Analogs with Neighboring Bistetrahydrofuran","volume":"36","author":"Sasaki","year":"1995","journal-title":"Tetrahedron Lett."},{"key":"ref_31","first-page":"1701","article-title":"Modulation of Matrix Metalloproteinase Activity by EDTA Prevents Posterior Capsular Opacification","volume":"18","author":"Hazra","year":"2012","journal-title":"Mol. Vis"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Beliz\u00e1rio, J., Vieira-Cordeiro, L., and Enns, S. (2019, January 30). Necroptotic Cell Death Signaling and Execution Pathway: Lessons from Knockout Mice. Available online: https:\/\/www.hindawi.com\/journals\/mi\/2015\/128076\/ref\/.","DOI":"10.1155\/2015\/128076"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4196\/kjpp.2020.24.6.493","article-title":"Apigenin Causes Necroptosis by Inducing ROS Accumulation, Mitochondrial Dysfunction, and ATP Depletion in Malignant Mesothelioma Cells","volume":"24","author":"Lee","year":"2020","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jcrpr.2017.12.001","article-title":"In Vitro Anticancer Assessments of Annona muricata Fractions and in vitro Antioxidant Profile of Fractions and Isolated Acetogenin (15-Acetyl Guanacone)","volume":"5","author":"Agu","year":"2018","journal-title":"J. Cancer Res. Pract."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10863-012-9489-1","article-title":"Mitochondrial Complex I Inhibitors, Acetogenins, Induce HepG2 Cell Death through the Induction of the Complete Apoptotic Mitochondrial Pathway","volume":"45","author":"Cautain","year":"2013","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Gomes, I.N.F., Silva-Oliveira, R.J., Oliveira Silva, V.A., Rosa, M.N., Vital, P.S., Barbosa, M.C.S., dos Santos, F.V., Junqueira, J.G.M., Severino, V.G.P., and Oliveira, B.G. (2019). Annona coriacea Mart. Fractions Promote Cell Cycle Arrest and Inhibit Autophagic Flux in Human Cervical Cancer Cell Lines. Molecules, 24.","DOI":"10.3390\/molecules24213963"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.canlet.2012.03.031","article-title":"Graviola: A Novel Promising Natural-Derived Drug That Inhibits Tumorigenicity and Metastasis of Pancreatic Cancer Cells in Vitro and in Vivo through Altering Cell Metabolism","volume":"323","author":"Torres","year":"2012","journal-title":"Cancer Lett."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Han, B., Wang, T.-D., Shen, S.-M., Yu, Y., Mao, C., Yao, Z.-J., and Wang, L.-S. (2015). Annonaceous Acetogenin Mimic AA005 Induces Cancer Cell Death via Apoptosis Inducing Factor through a Caspase-3-Independent Mechanism. BMC Cancer, 15.","DOI":"10.1186\/s12885-015-1133-0"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.biotechadv.2018.02.003","article-title":"In Vivo Therapeutic Applications of Cell Spheroids","volume":"36","author":"Ong","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e20201844","DOI":"10.1590\/0001-3765202220201844","article-title":"Dereplication by HPLC-ESI-MS and Antioxidant Activity of Phenolic Compounds from Banisteriopsis laevifolia (Malpighiaceae)","volume":"94","author":"Alexandre","year":"2022","journal-title":"An. Acad. Bras. Ci\u00eancias"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.tiv.2019.02.009","article-title":"WIN55,212-2 Induces Caspase-Independent Apoptosis on Human Glioblastoma Cells by Regulating HSP70, P53 and Cathepsin D","volume":"57","author":"Silva","year":"2019","journal-title":"Toxicol. Vitr."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ogbole, O.O., Segun, P.A., and Adeniji, A.J. (2017). In Vitro Cytotoxic Activity of Medicinal Plants from Nigeria Ethnomedicine on Rhabdomyosarcoma Cancer Cell Line and HPLC Analysis of Active Extracts. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-2005-8"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ismail, N., Ismail, M., Imam, M.U., Azmi, N.H., Fathy, S.F., Foo, J.B., and Abu Bakar, M.F. (2014). Mechanistic Basis for Protection of Differentiated SH-SY5Y Cells by Oryzanol-Rich Fraction against Hydrogen Peroxide-Induced Neurotoxicity. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-467"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"584","DOI":"10.14393\/BJ-v31n2a2015-23477","article-title":"Inhibition of Gelatinase Activity of MMP-2 and MMP-9 by Extracts of Bauhinia ungulata L.","volume":"31","author":"Nunes","year":"2015","journal-title":"Biosci. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1007\/s10637-019-00878-1","article-title":"Matteucinol, Isolated from Miconia Chamissois, Induces Apoptosis in Human Glioblastoma Lines via the Intrinsic Pathway and Inhibits Angiogenesis and Tumor Growth in Vivo","volume":"38","author":"Silva","year":"2020","journal-title":"Investig. New Drugs"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/0076-6879(67)10099-2","article-title":"[92a] Microsomal Lipid Peroxidation","volume":"Volume 10","author":"Ernster","year":"1967","journal-title":"Methods in Enzymology"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Foty, R. (2011). A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids. J. Vis. Exp., e2720.","DOI":"10.3791\/2720-v"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/9\/3809\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:26:24Z","timestamp":1760124384000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/9\/3809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,29]]},"references-count":47,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["molecules28093809"],"URL":"https:\/\/doi.org\/10.3390\/molecules28093809","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,29]]}}}