{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:35:20Z","timestamp":1761845720936,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European EC Horizon2020 TIMB3","award":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"],"award-info":[{"award-number":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation program","award":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"],"award-info":[{"award-number":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"]}]},{"name":"FC\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (FCT)","award":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"],"award-info":[{"award-number":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"]}]},{"name":"LS4FUTURE Associated Laboratory","award":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"],"award-info":[{"award-number":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"]}]},{"name":"CERM\/CIRMMP Italy Centre","award":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"],"award-info":[{"award-number":["810856","955740","UIDB\/04612\/2020","UIDP\/04612\/2020","AAC 01\/SAICT\/2016","PD\/BD\/135187\/2017","LA\/P\/0087\/2020","4509"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Rhodopseudomonas palustris is an alphaproteobacterium with impressive metabolic versatility, capable of oxidizing ferrous iron to fix carbon dioxide using light energy. Photoferrotrophic iron oxidation is one of the most ancient metabolisms, sustained by the pio operon coding for three proteins: PioB and PioA, which form an outer-membrane porin\u2013cytochrome complex that oxidizes iron outside of the cell and transfers the electrons to the periplasmic high potential iron\u2013sulfur protein (HIPIP) PioC, which delivers them to the light-harvesting reaction center (LH-RC). Previous studies have shown that PioA deletion is the most detrimental for iron oxidation, while, the deletion of PioC resulted in only a partial loss. The expression of another periplasmic HiPIP, designated Rpal_4085, is strongly upregulated in photoferrotrophic conditions, making it a strong candidate for a PioC substitute. However, it is unable to reduce the LH-RC. In this work we used NMR spectroscopy to map the interactions between PioC, PioA, and the LH-RC, identifying the key amino acid residues involved. We also observed that PioA directly reduces the LH-RC, and this is the most likely substitute upon PioC deletion. By contrast, Rpal_4085 demontrated significant electronic and structural differences from PioC. These differences likely explain its inability to reduce the LH-RC and highlight its distinct functional role. Overall, this work reveals the functional resilience of the pio operon pathway and further highlights the use of paramagnetic NMR for understanding key biological processes.<\/jats:p>","DOI":"10.3390\/molecules28124733","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T02:01:40Z","timestamp":1686708100000},"page":"4733","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protein Interactions in Rhodopseudomonas palustris TIE-1 Reveal the Molecular Basis for Resilient Photoferrotrophic Iron Oxidation"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6746-8455","authenticated-orcid":false,"given":"In\u00eas B.","family":"Trindade","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0577-9335","authenticated-orcid":false,"given":"Maria O.","family":"Firmino","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6709-9277","authenticated-orcid":false,"given":"Sander J.","family":"Noordam","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0501-3022","authenticated-orcid":false,"given":"Alexandra S.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]},{"given":"Bruno M.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9882-9754","authenticated-orcid":false,"given":"Mario","family":"Piccioli","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center, Department of Chemistry, University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2392-6450","authenticated-orcid":false,"given":"Ricardo O.","family":"Louro","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida da Rep\u00fablica (EAN), 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1472-4669.2005.00055.x","article-title":"The Potential Significance of Microbial Fe(III) Reduction during Deposition of Precambrian Banded Iron Formations","volume":"3","author":"Konhauser","year":"2005","journal-title":"Geobiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3389\/fmicb.2017.00323","article-title":"Photoferrotrophy: Remains of an Ancient Photosynthesis in Modern Environments","volume":"8","author":"Camacho","year":"2017","journal-title":"Front. 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