{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T09:29:58Z","timestamp":1770283798244,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT-Portuguese Foundation for Science and Technology","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"FCT-Portuguese Foundation for Science and Technology","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"FCT-Portuguese Foundation for Science and Technology","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]},{"name":"FCT-Portuguese Foundation for Science and Technology","award":["VA148P23"],"award-info":[{"award-number":["VA148P23"]}]},{"name":"AgrifoodXXI","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"AgrifoodXXI","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"AgrifoodXXI","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]},{"name":"AgrifoodXXI","award":["VA148P23"],"award-info":[{"award-number":["VA148P23"]}]},{"name":"Junta de Castilla y Le\u00f3n","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"Junta de Castilla y Le\u00f3n","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"Junta de Castilla y Le\u00f3n","award":["NORTE-01-0145-FEDER-000041"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000041"]}]},{"name":"Junta de Castilla y Le\u00f3n","award":["VA148P23"],"award-info":[{"award-number":["VA148P23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In recent years, nutmeg (Myristica fragans Houtt.) has attracted considerable attention in the field of phytochemistry due to its diverse array of bioactive compounds. However, the potential application of nutmeg as a biorational for crop protection has been insufficiently explored. This study investigated the constituents of a nutmeg hydroethanolic extract via gas chromatography-mass spectrometry and vibrational spectroscopy. The research explored the extract\u2019s activity against phytopathogenic fungi and oomycetes, elucidating its mechanism of action. The phytochemical profile revealed fatty acids (including tetradecanoic acid, 9-octadecenoic acid, n-hexadecanoic acid, dodecanoic acid, and octadecanoic acid), methoxyeugenol, and elemicin as the main constituents. Previously unreported phytochemicals included veratone, gelsevirine, and montanine. Significant radial growth inhibition of mycelia was observed against Botrytis cinerea, Colletotrichum acutatum, Diplodia corticola, Phytophthora cinnamomi, and especially against Fusarium culmorum. Mode of action investigation, involving Saccharomyces cerevisiae labeled positively with propidium iodide, and a mutant strain affected in ERG6, encoding sterol C-24 methyltransferase, suggested that the extract induces a necrotic type of death and targets ergosterol biosynthesis. The evidence presented underscores the potential of nutmeg as a source of new antimicrobial agents, showing particular promise against F. culmorum.<\/jats:p>","DOI":"10.3390\/molecules29020471","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T03:50:56Z","timestamp":1705549856000},"page":"471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Phytoconstituents and Ergosterol Biosynthesis-Targeting Antimicrobial Activity of Nutmeg (Myristica fragans Houtt.) against Phytopathogens"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9833-8550","authenticated-orcid":false,"given":"Adriana","family":"Cruz","sequence":"first","affiliation":[{"name":"Department of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Centre of Molecular and Environmental Biology (CBMA), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-3954","authenticated-orcid":false,"given":"Eva","family":"S\u00e1nchez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Forestry Engineering, ETSIIAA, University of Valladolid, Avenida de Madrid 44, 34004 Palencia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1854-0753","authenticated-orcid":false,"given":"Ana","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3989-8925","authenticated-orcid":false,"given":"Rui","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Centre of Molecular and Environmental Biology (CBMA), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0822-8248","authenticated-orcid":false,"given":"Ana","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Centre of Molecular and Environmental Biology (CBMA), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2713-2786","authenticated-orcid":false,"given":"Pablo","family":"Mart\u00edn-Ramos","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Forestry Engineering, ETSIIAA, University of Valladolid, Avenida de Madrid 44, 34004 Palencia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Brauer, V.S., Rezende, C.P., Pessoni, A.M., De Paula, R.G., Rangappa, K.S., Nayaka, S.C., Gupta, V.K., and Almeida, F. 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