{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T23:11:28Z","timestamp":1776813088667,"version":"3.51.2"},"reference-count":62,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["GHTMUID\/04413\/2020"],"award-info":[{"award-number":["GHTMUID\/04413\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA-REAL-LA\/P\/0117\/2020"],"award-info":[{"award-number":["LA-REAL-LA\/P\/0117\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of aqueous extracts of BITC and T. majus seeds, leaves, and stems. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of the chloroquine-susceptible Plasmodium falciparum 3D7 \u2212 GFP strain. Extracts and\/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7 \u2212 GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity that was higher in seeds than in leaves or stems. BITC\u2019s GI was comparable to chloroquine\u2019s. BITC\u2019s IC50 was similar in both strains; thus, a cross-resistance absence with aminoquinolines was found (RI &lt; 1). BITC presented features that could open new avenues for malaria drug discovery.<\/jats:p>","DOI":"10.3390\/molecules29102316","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T06:14:42Z","timestamp":1715753682000},"page":"2316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2301-5358","authenticated-orcid":false,"given":"Ana Maria","family":"Pint\u00e3o","sequence":"first","affiliation":[{"name":"Egas Moniz School of Health & Science, University Campus, Quinta da Granja Monte da Caparica, 2829-511 Caparica, Portugal"},{"name":"Egas Moniz Center for Interdisciplinary Research (CiiEM), Egas Moniz School of Health & Science, University Campus, Quinta da Granja Monte da Caparica, 2829-511 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3945-1880","authenticated-orcid":false,"given":"Tiago","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0313-0778","authenticated-orcid":false,"given":"F\u00e1tima","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"},{"name":"LAQV-REQUIMTE, MolSyn, IHMT, Universidade NOVA de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/5584_2019_428","article-title":"Quinolines and Quinolones as Antibacterial, Antifungal, Anti-Virulence, Antiviral and Anti-Parasitic Agents","volume":"Volume 1282","author":"Senerovic","year":"2020","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107658","DOI":"10.1016\/j.pharmthera.2020.107658","article-title":"The Birth of Artemisinin","volume":"216","author":"Ma","year":"2020","journal-title":"Pharmacol. 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