{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T09:31:16Z","timestamp":1774603876312,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["UIDB\/00772\/2020"],"award-info":[{"award-number":["UIDB\/00772\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDB\/00772\/2020"],"award-info":[{"award-number":["UIDB\/00772\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Wine production is one of the most important agricultural activities. The winemaking process generates a considerable volume of different residues characterized as by-products, such as pomace, seeds, stems, and skins. By-products are rich in polyphenols with antioxidant and antibacterial properties and may act as bacteriostatic or bactericidal agents against food-borne pathogens, improving food safety by enhancing antibiotic efficacy and reducing bacterial resistance. The aim of this study was to evaluate the phenolic composition and antioxidant activity of grape components (skins, seeds, and stems) from three red grape varieties (Periquita, Gamay, and Donzelinho Tinto) and determine their antibacterial activity against antibiotic-resistant bacteria, including Escherichia coli in food-producing animals and Listeria monocytogenes from food products and food-related environments. Ten phenolic compounds were quantified in these red grape varieties, with specific compounds found in different parts of the grape, including phenolic acids and flavonoids. Flavonoids are abundant in seeds and stems, malvidin-3-O-glucoside being the main anthocyanin in skins. The ethanolic extract from the seeds showed in vitro concentration-dependent activity against reactive species like \u2022NO and O2\u2022\u2212. Gamay extract was the most effective, followed by Donzelinho Tinto and Periquita. Extracts showed varying antibacterial activity against Gram-positive and Gram-negative bacteria, with stronger effects on Gram-positive bacteria. L. monocytogenes was more susceptible, while E. coli was limited to three strains. Seeds exhibited the strongest antibacterial activity, followed by stems. The results of our study provide evidence of the potential of grape by-products, particularly seeds, as sources of bioactive compounds with antioxidant and antibacterial properties, offering promising avenues for enhancing food safety and combating antibiotic resistance in food production and related environments.<\/jats:p>","DOI":"10.3390\/molecules29194708","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T10:20:52Z","timestamp":1728037252000},"page":"4708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Red Grape By-Products from the Demarcated Douro Region: Chemical Analysis, Antioxidant Potential and Antimicrobial Activity against Food-Borne Pathogens"],"prefix":"10.3390","volume":"29","author":[{"given":"Adriana","family":"Silva","sequence":"first","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2608-4601","authenticated-orcid":false,"given":"Raquel","family":"Martins","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u00e1rio de Farmacognosia, Departamento de Qui\u00e1mica, Faculdade de Farma\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9406-8433","authenticated-orcid":false,"given":"Vanessa","family":"Silva","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-0512","authenticated-orcid":false,"given":"F\u00e1tima","family":"Fernandes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u00e1rio de Farmacognosia, Departamento de Qui\u00e1mica, Faculdade de Farma\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Rosa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-8413","authenticated-orcid":false,"given":"Alfredo","family":"Aires","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-0735","authenticated-orcid":false,"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2742-0622","authenticated-orcid":false,"given":"Virg\u00edlio","family":"Falco","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u00e1rio de Farmacognosia, Departamento de Qui\u00e1mica, Faculdade de Farma\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0740-4396","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Valent\u00e3o","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u00e1rio de Farmacognosia, Departamento de Qui\u00e1mica, Faculdade de Farma\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"},{"name":"CECAV\u2014Veterinary and Animal Research Centre, University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Veterinary and Animal Research Centre, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tra\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Silva, A., Silva, V., Igrejas, G., Gaiv\u00e3o, I., Aires, A., Klibi, N., Enes Dapkevicius, M.D.L., Valent\u00e3o, P., Falco, V., and Poeta, P. (2021). Valorization of Winemaking By-Products as a Novel Source of Antibacterial Properties: New Strategies to Fight Antibiotic Resistance. Molecules, 26.","DOI":"10.3390\/molecules26082331"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Baiano, A. (2021). An Overview on Sustainability in the Wine Production Chain. Beverages, 7.","DOI":"10.3390\/beverages7010015"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ferrer-Gallego, R., and Silva, P. (2022). The Wine Industry By-Products: Applications for Food Industry and Health Benefits. Antioxidants, 11.","DOI":"10.3390\/antiox11102025"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Maicas, S., and Mateo, J.J. (2020). Sustainability of Wine Production. Sustainability, 12.","DOI":"10.3390\/su12020559"},{"key":"ref_5","first-page":"63","article-title":"Quantifying the sustainability of the wine sector through life cycle assessment (LCA)","volume":"7","author":"Bugaian","year":"2022","journal-title":"Econ. Contemp."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.fbp.2020.10.003","article-title":"Antioxidant and Antimicrobial Extracts Obtained from Agricultural By-Products: Strategies for a Sustainable Recovery and Future Perspectives","volume":"124","author":"Guerrini","year":"2020","journal-title":"Food Bioprod. Process."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s12576-022-00845-1","article-title":"Antioxidative Properties of Phenolic Compounds and Their Effect on Oxidative Stress Induced by Severe Physical Exercise","volume":"72","author":"Kruk","year":"2022","journal-title":"J. Physiol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"149","DOI":"10.17795\/jjnpp-15380","article-title":"Phenolic Compounds as Potential Antioxidant","volume":"8","author":"Pourreza","year":"2013","journal-title":"Jundishapur J. Nat. Pharm. Prod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7195125","DOI":"10.1155\/2021\/7195125","article-title":"Antioxidant Activity In Vitro Guided Screening and Identification of Flavonoids Antioxidants in the Extract from Tetrastigma hemsleyanum Diels et Gilg","volume":"2021","author":"Ding","year":"2021","journal-title":"Int. J. Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Gulcin, \u0130., and Alwasel, S.H. (2023). DPPH Radical Scavenging Assay. Processes, 11.","DOI":"10.3390\/pr11082248"},{"key":"ref_11","first-page":"609","article-title":"Antimicrobial, Antioxidant and Anticancer Properties of Globe Artichoke and Grape by-Products as a Source of the Bio-Active Phenolic Compounds","volume":"65","author":"Moselhy","year":"2023","journal-title":"Egypt. J. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Silva, A., Silva, V., Igrejas, G., Aires, A., Falco, V., Valent\u00e3o, P., and Poeta, P. (2023). Phenolic Compounds Classification and Their Distribution in Winemaking By-Products, Springer.","DOI":"10.1007\/s00217-022-04163-z"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sateriale, D., Forgione, G., Di Rosario, M., Pagliuca, C., Colicchio, R., Salvatore, P., Paolucci, M., and Pagliarulo, C. (2024). Vine-Winery Byproducts as Precious Resource of Natural Antimicrobials: In Vitro Antibacterial and Antibiofilm Activity of Grape Pomace Extracts against Foodborne Pathogens. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030437"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.3390\/molecules17021571","article-title":"Anthocyanins and Their Variation in Red Wines I. Monomeric Anthocyanins and Their Color Expression","volume":"17","author":"He","year":"2012","journal-title":"Molecules"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9057","DOI":"10.3390\/molecules15129057","article-title":"Biosynthesis of Anthocyanins and Their Regulation in Colored Grapes","volume":"15","author":"He","year":"2010","journal-title":"Molecules"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/S0031-9422(03)00438-2","article-title":"Analysis and Biological Activities of Anthocyanins","volume":"64","author":"Kong","year":"2003","journal-title":"Phytochemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/j.bcp.2013.06.010","article-title":"Malvidin-3-O-\u03b2 Glucoside, Major Grape Anthocyanin, Inhibits Human Macrophage-Derived Inflammatory Mediators and Decreases Clinical Scores in Arthritic Rats","volume":"86","author":"Decendit","year":"2013","journal-title":"Biochem. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6336","DOI":"10.1016\/j.bmcl.2013.09.071","article-title":"Gallic Acid is the Major Component of Grape Seed Extract That Inhibits Amyloid Fibril Formation","volume":"23","author":"Liu","year":"2013","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_19","first-page":"225","article-title":"Pharmacological Effects of Gallic Acid in Health and Disease: A Mechanistic Review","volume":"22","author":"Kahkeshani","year":"2019","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1080\/09637486.2016.1204595","article-title":"Vitis vinifera L. Cv Pinot Noir Pomace and Lees as Potential Sources of Bioactive Compounds","volume":"67","author":"Reis","year":"2016","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"De Luca, M., Restuccia, D., Spizzirri, U.G., Crupi, P., Ioele, G., Gorelli, B., Clodoveo, M.L., Saponara, S., and Aiello, F. (2023). Wine Lees as Source of Antioxidant Molecules: Green Extraction Procedure and Biological Activity. Antioxidants, 12.","DOI":"10.3390\/antiox12030622"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Padilla-Gonz\u00e1lez, G.F., Grosskopf, E., Sadgrove, N.J., and Simmonds, M.S.J. (2022). Chemical Diversity of Flavan-3-Ols in Grape Seeds: Modulating Factors and Quality Requirements. Plants, 11.","DOI":"10.3390\/plants11060809"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1007\/s10681-017-2032-z","article-title":"Flavan-3-Ols and Procyanidins in Grape Seeds: Biodiversity and Relationships among Wild and Cultivated Vines","volume":"213","author":"Trad","year":"2017","journal-title":"Euphytica"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1071\/FP03076","article-title":"UV Screening by Phenolics in Berries of Grapevine (Vitis vinifera)","volume":"30","author":"Kolb","year":"2003","journal-title":"Funct. Plant Biol."},{"key":"ref_25","first-page":"79","article-title":"Suicidality and Side Effects of Antidepressants and Antipsychotics","volume":"22","author":"Bodor","year":"2010","journal-title":"Psychiatr. Danub."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.foodchem.2017.08.117","article-title":"Antioxidant Properties of Catechins: Comparison with Other Antioxidants","volume":"241","author":"Grzesik","year":"2018","journal-title":"Food Chem."},{"key":"ref_27","first-page":"88","article-title":"Evaluation of Polyphenols Antioxidant Activity of Grape Seeds (V. vinifera)","volume":"31","author":"Gougoulias","year":"2008","journal-title":"Oxid. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chatterjee, S. (2016). Oxidative Stress, Inflammation, and Disease, Elsevier Inc.","DOI":"10.1016\/B978-0-12-803269-5.00002-4"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.foodcont.2018.05.031","article-title":"Chemical Composition, Antioxidant and Antimicrobial Activity of Phenolic Compounds Extracted from Wine Industry by-Products","volume":"92","author":"Silva","year":"2018","journal-title":"Food Control"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Pozzo, L., Grande, T., Raffaelli, A., Longo, V., Weidner, S., Amarowicz, R., and Karama\u0107, M. (2023). Characterization of Antioxidant and Antimicrobial Activity and Phenolic Compound Profile of Extracts from Seeds of Different Vitis Species. Molecules, 28.","DOI":"10.3390\/molecules28134924"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1007\/s11694-022-01289-2","article-title":"Biological Activities of Egyptian Grape and Mulberry By-Products and Their Potential Use as Natural Sources of Food Additives and Nutraceuticals Foods","volume":"16","author":"Mattar","year":"2022","journal-title":"J. Food Meas. Charact."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"de Andrade, R.B., Machado, B.A.S., Barreto, G.d.A., Nascimento, R.Q., Corr\u00eaa, L.C., Leal, I.L., Tavares, P.P.L.G., Ferreira, E.d.S., and Umsza-Guez, M.A. (2021). Syrah Grape Skin Residues Has Potential as Source of Antioxidant and Anti-Microbial Bioactive Compounds. Biology, 10.","DOI":"10.3390\/biology10121262"},{"key":"ref_33","first-page":"39","article-title":"Antimicrobial Effects of Grape and Pomegranate Waste Extracts against Two Foodborne Pathogens","volume":"10","author":"Javanmard","year":"2020","journal-title":"J. Food Biosci. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Caponio, G.R., Noviello, M., Calabrese, F.M., Gambacorta, G., Giannelli, G., and De Angelis, M. (2022). Effects of Grape Pomace Polyphenols and In Vitro Gastrointestinal Digestion on Antimicrobial Activity: Recovery of Bioactive Compounds. Antioxidants, 11.","DOI":"10.3390\/antiox11030567"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Krasteva, D., Ivanov, Y., Chengolova, Z., and Godjevargova, T. (2023). Antimicrobial Potential, Antioxidant Activity, and Phenolic Content of Grape Seed Extracts from Four Grape Varieties. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020395"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kalli, E., Lappa, I., Bouchagier, P., Tarantilis, P.A., and Skotti, E. (2018). Novel Application and Industrial Exploitation of Winery By-Products. Bioresour. Bioprocess., 5.","DOI":"10.1186\/s40643-018-0232-6"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, L.A., Lorenzo, J.M., and Trindade, M.A. (2021). Fruit and Agro-Industrial Waste Extracts as Potential Antimicrobials in Meat Products: A Brief Review. Foods, 10.","DOI":"10.3390\/foods10071469"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Costa, M.M., Alfaia, C.M., Lopes, P.A., Pestana, J.M., and Prates, J.A.M. (2022). Grape By-Products as Feedstuff for Pig and Poultry Production. Animals, 12.","DOI":"10.3390\/ani12172239"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Simitzis, P.E., and Deligeorgis, S.G. (2018). Agroindustrial By-Products and Animal Products: A Great Alternative for Improving Food-Quality Characteristics and Preserving Human Health, Elsevier Inc.","DOI":"10.1016\/B978-0-12-811442-1.00008-0"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tifs.2019.02.010","article-title":"Pomegranate and Grape By-Products and Their Active Compounds: Are They a Valuable Source for Food Applications?","volume":"86","author":"Andrade","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.foodchem.2015.07.142","article-title":"Identification of Vitis vinifera L. Grape Berry Skin Color Mutants and Polyphenolic Profile","volume":"194","author":"Ferreira","year":"2016","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1016\/j.talanta.2008.01.012","article-title":"Principal Components of Phenolics to Characterize Red Vinho Verde Grapes: Anthocyanins or Non-Coloured Compounds?","volume":"75","author":"Fique","year":"2008","journal-title":"Talanta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"127456","DOI":"10.1016\/j.foodchem.2020.127456","article-title":"In Vitro Multifunctionality of Phlorotannin Extracts from Edible Fucus Species on Targets Underpinning Neurodegeneration","volume":"333","author":"Barbosa","year":"2020","journal-title":"Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1007\/s10811-019-01816-7","article-title":"Phlorotannins from Fucales: Potential to Control Hyperglycemia and Diabetes-Related Vascular Complications","volume":"31","author":"Lopes","year":"2019","journal-title":"J. Appl. Phycol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Pereira, R.B., Pereira, D.M., Jim, C., Rodr, J., Nieto, R.M., Videira, R.A., Silva, O., Andrade, P.B., and Valent\u00e3o, P. (2019). Anti-inflammatory effects of 5\u03b1, 8\u03b1-epidioxycholest-6-en-3\u03b2-ol, a steroidal endoperoxide isolated from Aplysia depilans, based on bioguided fractionation and NMR analysis. Mar. Drugs, 17.","DOI":"10.3390\/md17060330"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Silva, A., Silva, V., Dapkevicius, M.d.L.E., Azevedo, M., Cordeiro, R., Pereira, J.E., Valent\u00e3o, P., Falco, V., Igrejas, G., and Cani\u00e7a, M. (2024). Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in E. coli Isolated from Healthy Swine in Portugal. Pathogens, 13.","DOI":"10.3390\/pathogens13040305"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Silva, A., Silva, V., Tavares, T., L\u00f3pez, M., Rojo-Bezares, B., Pereira, J.E., Falco, V., Valent\u00e3o, P., Igrejas, G., and S\u00e1enz, Y. (2024). Rabbits as a Reservoir of Multidrug-Resistant Escherichia coli: Clonal Lineages and Public Health Impact. Antibiotics, 13.","DOI":"10.3390\/antibiotics13040376"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Silva, A., Silva, V., Gomes, J.P., Coelho, A., Batista, R., Saraiva, C., Esteves, A., Martins, \u00c2., Contente, D., and Diaz-Formoso, L. (2024). Listeria Monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights. Antibiotics, 13.","DOI":"10.3390\/antibiotics13050447"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/19\/4708\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:10:45Z","timestamp":1760112645000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/19\/4708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,4]]},"references-count":48,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["molecules29194708"],"URL":"https:\/\/doi.org\/10.3390\/molecules29194708","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,4]]}}}