{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:25:35Z","timestamp":1777497935594,"version":"3.51.4"},"reference-count":90,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Science and Technology (FCT)","award":["PRIMA\/0001\/2020"],"award-info":[{"award-number":["PRIMA\/0001\/2020"]}]},{"name":"Foundation for Science and Technology (FCT)","award":["UIDB\/00211\/2020"],"award-info":[{"award-number":["UIDB\/00211\/2020"]}]},{"name":"FCT\/MCTES","award":["PRIMA\/0001\/2020"],"award-info":[{"award-number":["PRIMA\/0001\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDB\/00211\/2020"],"award-info":[{"award-number":["UIDB\/00211\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Flaxseeds, which have been consumed for thousands of years, have recently gained increasing popularity due to their rich composition, including omega-3 fatty acids, lignans, proteins, and fibers. These components are strongly associated with various health benefits, such as improving cardiovascular health, preventing certain types of cancer, controlling diabetes, promoting gastro-intestinal well-being, and aiding in weight management. This monograph explores the role of flaxseeds in nutrition, as well as their potential risks. Despite their numerous health benefits, flaxseeds also represent concerns due to excessive consumption and possible contamination, particularly from cyanogenic glycosides. Therefore, the levels of these compounds must be controlled, and this monograph also analyzes the available methods to detect and reduce these contaminants, ensuring the safety of flaxseed and flaxseed products consumers. Flaxseed is considered a valuable addition when incorporated into the diet, but it is necessary to continue research and promote technological improvements to maximize their benefits and minimize their risks.<\/jats:p>","DOI":"10.3390\/molecules30061335","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T04:29:28Z","timestamp":1742185768000},"page":"1335","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Flaxseed in Diet: A Comprehensive Look at Pros and Cons"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1053-7957","authenticated-orcid":false,"given":"Sara","family":"Duarte","sequence":"first","affiliation":[{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3471-184X","authenticated-orcid":false,"given":"Muhammad Ajmal","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Hazara University, Mansehra 21300, Pakistan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0226-921X","authenticated-orcid":false,"given":"Ana","family":"Sanches Silva","sequence":"additional","affiliation":[{"name":"University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"},{"name":"Centre for Animal Science Studies (CECA), Instituto de Ci\u00eancias e Tecnologias Agr\u00e1rias e Agro-Alimentares (ICETA), University of Porto, 4501-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (Al4AnimalS), 1300-477 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1043-4526(06)51001-0","article-title":"Flaxseed","volume":"51","author":"Hall","year":"2006","journal-title":"Adv. Food Nutr. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Parikh, M., Maddaford, T.G., Austria, J.A., Aliani, M., Netticadan, T., and Pierce, G.N. (2019). Dietary Flaxseed as a Strategy for Improving Human Health. Nutrients, 11.","DOI":"10.3390\/nu11051171"},{"key":"ref_3","unstructured":"(2024, February 28). Canadian Food Inspection Agency The Biology of Linum usitatissimum L. (Flax). Available online: https:\/\/inspection.canada.ca\/en\/plant-varieties\/plants-novel-traits\/applicants\/directive-94-08\/biology-documents\/linum-usitatissimum-flax."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100573","DOI":"10.1016\/j.focha.2023.100573","article-title":"Flaxseed (Linum usitatissimum); Phytochemistry, Pharmacological Characteristics and Functional Food Applications","volume":"4","author":"Kauser","year":"2024","journal-title":"Food Chem. Adv."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1007\/s10722-010-9650-9","article-title":"Genetic Evidence for Early Flax Domestication with Capsular Dehiscence","volume":"58","author":"Fu","year":"2011","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_6","unstructured":"Cabi Digital Library (2019). Linum usitatissimum (Flax). CABI Compendium, CABI Head Office."},{"key":"ref_7","unstructured":"(2024, August 05). FAOSTAT. Available online: https:\/\/www.fao.org\/faostat\/en\/#data\/QCL\/visualize."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1080\/87559129.2023.2209794","article-title":"Risk\/Benefits of the Use of Chia, Quinoa, Sesame and Flax Seeds in Bakery Products. An Update Review","volume":"40","author":"Olombrada","year":"2024","journal-title":"Food Rev. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"21443","DOI":"10.1007\/s13399-023-04297-4","article-title":"Production of Gluten-Free Biscuits with Inulin and Flaxseed Powder: Investigation of Physicochemical Properties and Formulation Optimization","volume":"14","author":"Nasiri","year":"2024","journal-title":"Biomass Convers. Biorefin."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/s10973-011-1638-y","article-title":"Influence of Toasting on the Nutritious and Thermal Properties of Flaxseed","volume":"106","author":"Epaminondas","year":"2011","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_11","unstructured":"(2024, August 02). INSA_pt. Available online: https:\/\/jspapp.test.insa.foodcase-services.com\/foodcomp\/food?23745."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"115011","DOI":"10.1016\/j.indcrop.2022.115011","article-title":"Anticipating Global Warming Effects: A Comprehensive Study of Drought Impact of Both Flax Plants and Fibres","volume":"184","author":"Melelli","year":"2022","journal-title":"Ind. Crops Prod."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zare, S., Mirlohi, A., Sabzalian, M.R., Saeidi, G., Ko\u00e7ak, M.Z., and Hano, C. (2023). Water Stress and Seed Color Interacting to Impact Seed and Oil Yield, Protein, Mucilage, and Secoisolariciresinol Diglucoside Content in Cultivated Flax (Linum usitatissimum L.). Plants, 12.","DOI":"10.3390\/plants12081632"},{"key":"ref_14","first-page":"83","article-title":"Variation in Physico-Chemical\/Analytical Characteristics of Oil among Different Flaxseed (Linum usittatissimum L.) Cultivars","volume":"28","author":"Yaqoob","year":"2016","journal-title":"Ital. J. Food Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ocsci.2020.08.001","article-title":"Comparison of Fatty Acid Composition, Phytochemical Profile and Antioxidant Activity in Four Flax (Linum usitatissimum L.) Varieties","volume":"5","author":"Qiu","year":"2020","journal-title":"Oil Crop Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.plipres.2016.07.002","article-title":"Metabolism and Functional Effects of Plant-Derived Omega-3 Fatty Acids in Humans","volume":"64","author":"Baker","year":"2016","journal-title":"Prog. Lipid Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1002\/fsn3.3165","article-title":"Antioxidant Activity and Phytochemical Analysis of Fennel Seeds and Flaxseed","volume":"11","author":"Noreen","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_18","first-page":"4","article-title":"Nutritional value and functional properties of flaxseed","volume":"81","author":"Martinchik","year":"2012","journal-title":"Vopr. Pitan."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Plaha, N.S., Awasthi, S., Sharma, A., and Kaushik, N. (2022). Distribution, Biosynthesis and Therapeutic Potential of Lignans. 3 Biotech, 12.","DOI":"10.1007\/s13205-022-03318-9"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1007\/s13197-013-1247-9","article-title":"Flax and Flaxseed Oil: An Ancient Medicine & Modern Functional Food","volume":"51","author":"Goyal","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jff.2015.06.053","article-title":"Flaxseed Lignan Secoisolariciresinol Diglucoside Improves Insulin Sensitivity through Upregulation of GLUT4 Expression in Diet-Induced Obese Mice","volume":"18","author":"Wang","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00547-004-1060-4","article-title":"Antihypertensive Activity of Secoisolariciresinol Diglucoside (SDG) Isolated from Flaxseed: Role of Guanylate Cyclase","volume":"13","author":"Prasad","year":"2011","journal-title":"Int. J. Angiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.bcab.2017.11.017","article-title":"Flaxseed: Composition, Detoxification, Utilization, and Opportunities","volume":"13","author":"Bekhit","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/BF00023854","article-title":"Flavonoid Content of Flaxseed. Influence of Cultivar and Environment","volume":"90","author":"Mazza","year":"1996","journal-title":"Euphytica"},{"key":"ref_25","unstructured":"(2024, March 06). Associa\u00e7\u00e3o Portuguesa de Nutri\u00e7\u00e3o\u2014Dislipidemias: Caracteriza\u00e7\u00e3o e Tratamento Nutricional. Available online: https:\/\/www.apn.org.pt\/documentos\/ebooks\/Ebook_Dislipidemias_CaracterizacaoETratamentoNutricional.pdf."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"H146","DOI":"10.1152\/ajpheart.00400.2017","article-title":"Flaxseed: Its Bioactive Components and Their Cardiovascular Benefits","volume":"314","author":"Parikh","year":"2018","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"H903","DOI":"10.1152\/ajpheart.00201.2017","article-title":"Dietary Modulation of Oxylipins in Cardiovascular Disease and Aging","volume":"313","author":"Caligiuri","year":"2017","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1161\/HYPERTENSIONAHA.113.02094","article-title":"Potent Antihypertensive Action of Dietary Flaxseed in Hypertensive Patients","volume":"62","author":"Weighell","year":"2013","journal-title":"Hypertension"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"749","DOI":"10.3945\/jn.114.204594","article-title":"Dietary Flaxseed Independently Lowers Circulating Cholesterol and Lowers It beyond the Effects of Cholesterol-Lowering Medications Alone in Patients with Peripheral Artery Disease","volume":"145","author":"Edel","year":"2015","journal-title":"J. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3945\/ajcn.2009.27469","article-title":"Meta-Analysis of the Effects of Flaxseed Interventions on Blood Lipids","volume":"90","author":"Pan","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"H2394","DOI":"10.1152\/ajpheart.01104.2006","article-title":"Dietary Flaxseed Inhibits Atherosclerosis in the LDL Receptor-Deficient Mouse in Part through Antiproliferative and Anti-Inflammatory Actions","volume":"293","author":"Dupasquier","year":"2007","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"H1743","DOI":"10.1152\/ajpheart.00606.2012","article-title":"Effects of Dietary Flaxseed on Atherosclerotic Plaque Regression","volume":"304","author":"Francis","year":"2013","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"H2987","DOI":"10.1152\/ajpheart.01179.2005","article-title":"Effects of Dietary Flaxseed on Vascular Contractile Function and Atherosclerosis during Prolonged Hypercholesterolemia in Rabbits","volume":"291","author":"Dupasquier","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1017\/S0007114507871649","article-title":"Dietary Flaxseed Lignan Extract Lowers Plasma Cholesterol and Glucose Concentrations in Hypercholesterolaemic Subjects","volume":"99","author":"Zhang","year":"2008","journal-title":"Br. J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1080\/07315724.2013.791147","article-title":"Lignan Content of the Flaxseed Influences Its Biological Effects in Healthy Men and Women","volume":"32","author":"Almario","year":"2013","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1161\/ATVBAHA.111.226118","article-title":"Dietary \u03b1-Linolenic Acid Inhibits Arterial Thrombus Formation, Tissue Factor Expression, and Platelet Activation","volume":"31","author":"Holy","year":"2011","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1093\/jn\/134.12.3250","article-title":"Dietary Flaxseed Protects against Ventricular Fibrillation Induced by Ischemia-Reperfusion in Normal and Hypercholesterolemic Rabbits","volume":"134","author":"Ander","year":"2004","journal-title":"J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.arcmed.2011.04.008","article-title":"\u03b1-Linolenic Acid Intake Attenuates Myocardial Ischemia\/Reperfusion Injury through Anti-Inflammatory and Anti-Oxidative Stress Effects in Diabetic but Not Normal Rats","volume":"42","author":"Xie","year":"2011","journal-title":"Arch. Med. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jjcc.2015.08.002","article-title":"Cardioprotective Mechanism of Omega-3 Polyunsaturated Fatty Acids","volume":"67","author":"Endo","year":"2016","journal-title":"J. Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105816","DOI":"10.1016\/j.jff.2023.105816","article-title":"The Effect of Flaxseed Supplementation on Glycemic Control in Adults: An Updated Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"110","author":"Kavyani","year":"2023","journal-title":"J. Funct. Foods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s12944-019-1167-4","article-title":"Dietary Flaxseed Oil Rich in Omega-3 Suppresses Severity of Type 2 Diabetes Mellitus via Anti-Inflammation and Modulating Gut Microbiota in Rats","volume":"19","author":"Zhu","year":"2020","journal-title":"Lipids Health Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.nutres.2022.12.008","article-title":"Flaxseed Supplementation Significantly Reduces Hemoglobin A1c in Patients with Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis","volume":"110","author":"Xi","year":"2023","journal-title":"Nutr. Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Calado, A., Neves, P.M., Santos, T., and Ravasco, P. (2018). The Effect of Flaxseed in Breast Cancer: A Literature Review. Front. Nutr., 5.","DOI":"10.3389\/fnut.2018.00004"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1207\/S15327914NC330211","article-title":"Effect of Flaxseed Consumption on Urinary Estrogen Metabolites in Postmenopausal Women","volume":"33","author":"Haggans","year":"1999","journal-title":"Nutr. Cancer"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1158\/1078-0432.CCR-04-2326","article-title":"Dietary Flaxseed Alters Tumor Biological Markers in Postmenopausal Breast Cancer","volume":"11","author":"Thompson","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1080\/01635581.2014.894097","article-title":"A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers","volume":"66","author":"McCann","year":"2014","journal-title":"Nutr. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Mueed, A., Shibli, S., Korma, S.A., Madjirebaye, P., Esatbeyoglu, T., and Deng, Z. (2022). Flaxseed Bioactive Compounds: Chemical Composition, Functional Properties, Food Applications and Health Benefits-Related Gut Microbes. Foods, 11.","DOI":"10.3390\/foods11203307"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.jff.2018.05.042","article-title":"Flaxseed Gum Reduces Body Weight by Regulating Gut Microbiota","volume":"47","author":"Luo","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.2147\/JMDH.S379708","article-title":"Influence of Flax Seeds on the Gut Microbiota of Elderly Patients with Constipation","volume":"15","author":"Ma","year":"2022","journal-title":"J. Multidiscip. Healthc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/07315724.2008.10719693","article-title":"Bioavailability of Alpha-Linolenic Acid in Subjects after Ingestion of Three Different Forms of Flaxseed","volume":"27","author":"Austria","year":"2008","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3923\/ajpnft.2011.1.22","article-title":"Phytic Acid in Cereal Grains: Structure, Healthy or Harmful Ways to Reduce Phytic Acid in Cereal Grains and Their Effects on Nutritional Quality","volume":"1","author":"Coulibaly","year":"2010","journal-title":"Am. J. Plant Nutr. Fertil. Technol."},{"key":"ref_52","unstructured":"Nissar, J., Ahad, T., Naik, H.R., and Hussain, S.K. (2024, May 15). A Review Phytic Acid: As Antinutrient or Nutraceutical. Available online: https:\/\/www.phytojournal.com\/archives\/2017\/vol6issue6\/PartV\/6-6-208-319.pdf."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e15344","DOI":"10.1111\/jfpp.15344","article-title":"Influence of Sprouting on Proximate Composition, Dietary Fiber, Nutrient Availability, Antinutrient, and Antioxidant Activity of Flaxseed Varieties","volume":"45","author":"Khare","year":"2021","journal-title":"J. Food Process. Preserv."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/1750-3841.13985","article-title":"Inactivation Methods of Trypsin Inhibitor in Legumes: A Review","volume":"83","year":"2018","journal-title":"J. Food Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1093\/jn\/116.5.920","article-title":"Trypsin Inhibitors: Concern for Human Nutrition or Not?","volume":"116","author":"Liener","year":"1986","journal-title":"J. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Dzuvor, C.K.O., Taylor, J.T., Acquah, C., Pan, S., and Agyei, D. (2018). Bioprocessing of Functional Ingredients from Flaxseed. Molecules, 23.","DOI":"10.3390\/molecules23102444"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Vetter, J. (2017). Plant Cyanogenic Glycosides. Plant Toxins, Springer.","DOI":"10.1007\/978-94-007-6464-4_19"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1007\/s13197-014-1293-y","article-title":"Flaxseed-a Potential Functional Food Source","volume":"52","author":"Kajla","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"127305","DOI":"10.1016\/j.foodchem.2020.127305","article-title":"Development and Validation of Eight Cyanogenic Glucosides via Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry in Agri-Food","volume":"331","author":"Zhong","year":"2020","journal-title":"Food Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.chroma.2019.04.072","article-title":"Development and Validation of an Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry Method for Simultaneous Quantification of Cyanogenic Glycosides and Secoisolariciresinol Diglucoside in Flaxseed (Linum usitatissimum L.)","volume":"1601","author":"Zhao","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"7011","DOI":"10.1007\/s00216-017-0637-7","article-title":"Use of qNMR for Speciation of Flaxseeds (Linum usitatissimum) and Quantification of Cyanogenic Glycosides","volume":"409","author":"Roulard","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"131962","DOI":"10.1016\/j.foodchem.2021.131962","article-title":"Highly Sensitive Analysis of Cyanogenic Glycosides in Cold-Pressed Flaxseed Oil by Employing Cigarette Filter Fiber-Based SPE Coupled with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry","volume":"377","author":"Cai","year":"2022","journal-title":"Food Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1021\/jf950838j","article-title":"Determination of Cyanogenic Glycosides in Flaxseed by Barbituric Acid\u2212Pyridine, Pyridine\u2212Pyrazolone, and High-Performance Liquid Chromatography Methods","volume":"44","author":"Kobaisy","year":"1996","journal-title":"J. Agric. Food Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1021\/jf00020a010","article-title":"Cyanogenic Compounds in Flaxseed","volume":"40","author":"Oomah","year":"1992","journal-title":"J. Agric. Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Shahwar, D., Young, L.W., Shim, Y.Y., and Reaney, M.J.T. (2019). Extractive Silylation Method for High Throughput GC Analysis of Flaxseed Cyanogenic Glycosides. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 1132.","DOI":"10.1016\/j.jchromb.2019.121816"},{"key":"ref_66","unstructured":"(2024, July 12). EURL-MP-Method_010 Cyanogenic Glucosides (CNGs) in Food and Feed by LC-MSMS. WFSR Wageningen University & Research, 2023, Version 1. Available online: https:\/\/www.wur.nl\/en\/show\/eurlmp-method_010-cyanogenic-glucosides-cngs-in-food-and-feed-by-lc-msms-v1.htm."},{"key":"ref_67","unstructured":"(2024, September 02). Water Corporation\u2014Acquity UPLC Columns. Available online: https:\/\/imchem.fr\/assets\/files\/Waters\/ACQUITY_UPLC_%202012%20720001140en.pdf."},{"key":"ref_68","unstructured":"(2024, September 02). Agilent\u2014ZORBAX Part Number 959757-902. Available online: https:\/\/www.agilent.com\/store\/pt_BR\/Prod-959757-902\/959757-902."},{"key":"ref_69","unstructured":"(2024, September 02). Waters Corporation\u2014How to Choose a Column. Available online: https:\/\/www.waters.com\/webassets\/cms\/library\/docs\/lcHowToChooseAColumn.pdf."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"143","DOI":"10.5487\/TR.2013.29.2.143","article-title":"Determination of Cyanogenic Compounds in Edible Plants by Ion Chromatography","volume":"29","author":"Cho","year":"2013","journal-title":"Toxicol. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e23059","DOI":"10.1590\/s2175-97902023e23059","article-title":"Determination of Cyanide in Whole Seeds and Brans of Linseed (Linum usitatissimum Linn) by Molecular Spectrophotometry","volume":"59","author":"Pereira","year":"2023","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Erkekoglu, P., and Ogawa, T. (2021). Toxicity Potential of Cyanogenic Glycosides in Edible Plants. Medical Toxicology, IntechOpen.","DOI":"10.5772\/intechopen.77665"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM), Schrenk, D., Bignami, M., Bodin, L., Chipman, J.K., Del Mazo, J., Grasl-Kraupp, B., Hogstrand, C., Hoogenboom, L.R., and Leblanc, J.-C. (2019). Evaluation of the Health Risks Related to the Presence of Cyanogenic Glycosides in Foods Other than Raw Apricot Kernels. EFSA J., 17, e05662.","DOI":"10.2903\/j.efsa.2019.5662"},{"key":"ref_74","unstructured":"(2024, July 21). Regulation-2023\/915-EN-EUR-Lex. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2023\/915\/oj."},{"key":"ref_75","unstructured":"(2024, August 06). RASFF. Available online: https:\/\/food.ec.europa.eu\/safety\/rasff_en."},{"key":"ref_76","unstructured":"(2024, August 08). European Commission\u2014RASFF Window. Notification 2022.2641: Increased Cyanide Content in Organic Flaxseed from Germany. Available online: https:\/\/webgate.ec.europa.eu\/rasff-window\/screen\/notification\/547009."},{"key":"ref_77","unstructured":"(2024, August 08). European Commission\u2014RASFF Window. Notification 2023.0750: Hydrocyanic Acid (HCN) in Organic Blond Flaxseed from T\u00fcrkiye. Available online: https:\/\/webgate.ec.europa.eu\/rasff-window\/screen\/notification\/593771."},{"key":"ref_78","unstructured":"(2024, August 08). European Commission\u2014RASFF Window. Notification 2024.2851: Hydrocyanic Acid in Flaxseed from Poland. Available online: https:\/\/webgate.ec.europa.eu\/rasff-window\/screen\/notification\/677295."},{"key":"ref_79","unstructured":"(2024, August 08). European Commission\u2014RASFF Window. Notification 2024.3101: Hydrocyanic Acid in Flaxseed from Belgium. Available online: https:\/\/webgate.ec.europa.eu\/rasff-window\/screen\/notification\/679173."},{"key":"ref_80","unstructured":"(2024, August 08). European Commission\u2014RASFF Window. Notification 2024.3166: Hydrocyanic Acid in Organic Brown Flaxseed from India. Available online: https:\/\/webgate.ec.europa.eu\/rasff-window\/screen\/notification\/679478."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"e13406","DOI":"10.1111\/jfpe.13406","article-title":"Structural and Functional Properties of Raw and Defatted Flaxseed Flour and Degradation of Cynogenic Contents Using Different Processing Methods","volume":"43","author":"Safdar","year":"2020","journal-title":"J. Food Process Eng."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.seppur.2007.09.016","article-title":"Extrusion Detoxification Technique on Flaxseed by Uniform Design Optimization","volume":"61","author":"Wu","year":"2008","journal-title":"Sep. Purif. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1080\/19440049.2012.680202","article-title":"An Efficient Fermentation Method for the Degradation of Cyanogenic Glycosides in Flaxseed","volume":"29","author":"Wu","year":"2012","journal-title":"Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1007\/s11746-015-2729-x","article-title":"Effect of Extraction Method on the Phenolic and Cyanogenic Glucoside Profile of Flaxseed Extracts and Their Antioxidant Capacity","volume":"92","author":"Waszkowiak","year":"2015","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM), Knutsen, H.K., Alexander, J., Barreg\u00e5rd, L., Bignami, M., Br\u00fcschweiler, B., Ceccatelli, S., Cottrill, B., Dinovi, M., and Edler, L. (2017). Assessment of a Decontamination Process for Hydrocyanic Acid in Linseed Intended for Use in Animal Feed. EFSA J., 15, e05004.","DOI":"10.2903\/j.efsa.2017.5004"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1111\/ijfs.14098","article-title":"Effects of Germination on Tocopherol, Secoisolarlciresinol Diglucoside, Cyanogenic Glycosides and Antioxidant Activities in Flaxseed (Linum usitatissimum L.)","volume":"54","author":"Li","year":"2019","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"134441","DOI":"10.1016\/j.foodchem.2022.134441","article-title":"Depletion of Cyanogenic Glycosides in Whole Flaxseed via Lactobacillaceae Fermentation","volume":"403","author":"Huang","year":"2023","journal-title":"Food Chem."},{"key":"ref_88","first-page":"1456","article-title":"Variation in the Content of Cyanogenic Glycosides in Flaxseed Meal from Twenty-One Varieties","volume":"5","author":"Russo","year":"2014","journal-title":"Food Nutr. Sci."},{"key":"ref_89","first-page":"127","article-title":"Results of Linseed Breeding in the Czech Republic","volume":"61","author":"Pavelek","year":"2011","journal-title":"Tag. Ver. Pfl Anzenz\u00fcchter Saatgutkaufl Eute Osterr."},{"key":"ref_90","first-page":"214","article-title":"Application of a Comprehensive Metabolomics Approach for the Selection of Flaxseed Varieties with the Highest Nutritional and Medicinal Attributes","volume":"29","author":"Salem","year":"2021","journal-title":"J. Food Drug Anal."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/6\/1335\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:54:40Z","timestamp":1760028880000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/6\/1335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,16]]},"references-count":90,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["molecules30061335"],"URL":"https:\/\/doi.org\/10.3390\/molecules30061335","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,16]]}}}