{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:11:36Z","timestamp":1760058696809,"version":"build-2065373602"},"reference-count":70,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Funds by FCT\u2014Portuguese Foundation for Science and Technology","award":["UID\/04033","LA\/P\/0126\/2020"],"award-info":[{"award-number":["UID\/04033","LA\/P\/0126\/2020"]}]},{"name":"Centro de Investiga\u00e7\u00e3o e de Tecnologias Agro-Ambientais e Biol\u00f3gicas","award":["UID\/04033","LA\/P\/0126\/2020"],"award-info":[{"award-number":["UID\/04033","LA\/P\/0126\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Honey is a multifaceted substance whose composition is intricately affected by various biotic and abiotic elements generated in the bee colony\u2019s surroundings, including botanical and geographical origins, climatic conditions, soil characteristics, and beekeeping techniques. Monofloral honeys are identified by pollen analysis and are derived from the nectar of a predominant plant species, exhibiting rich sensory and nutritional profiles, making them food matrices with unique characteristics and excellent qualities. To explore the monofloral honey potential harvested in different regions of Portugal, a comprehensive study was conducted including the determination of phenolic composition and the assessment of biological activities. In addition to this evaluation, the inter simple sequence repeat (ISSR) was used to help differentiate honeys by botanical origin. The phenolic content and the antioxidant capacity were evaluated by spectrophotometric methods, observing, in general, differences between monofloral honeys. The honey from Citrus sinensis (Silves) exhibited the lowest phenolic content, including total phenols, ortho-diphenols, and flavonoids, whereas honeydew (Vinhais) showed the highest values. Regarding the antioxidant capacity, honey from Lavandula stoechas (Almod\u00f4var) presented the lowest values, while honeydew (Vinhais) displayed the highest values for both DPPH and FRAP assays. In relation to the ABTS assay, the honey from Metrosideros excelsa (Aveiro) exhibited the lowest values, whereas the honey from Eucalyptus spp. (Arouca) showed the highest. The ISSR marker analysis allows the distribution of the samples based on the honey\u2019s botanical origin, suggesting its potential role in honey authentication.<\/jats:p>","DOI":"10.3390\/molecules30081808","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T20:05:56Z","timestamp":1744920356000},"page":"1808","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Portuguese Monofloral Honeys: Molecular Insights and Biochemical Characterization"],"prefix":"10.3390","volume":"30","author":[{"given":"Mariana","family":"Silva","sequence":"first","affiliation":[{"name":"Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2215-5682","authenticated-orcid":false,"given":"Miguel","family":"Maia","sequence":"additional","affiliation":[{"name":"APISMAIA, Produtos & Servi\u00e7os, 4490-463 P\u00f3voa de Varzim, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1108-8389","authenticated-orcid":false,"given":"M\u00e1rcia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5834-6141","authenticated-orcid":false,"given":"Ana Novo","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.jscs.2012.11.009","article-title":"Mineral Content and Physical Properties of Local and Imported Honeys in Saudi Arabia","volume":"18","author":"Alqarni","year":"2014","journal-title":"J. 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