{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:15:19Z","timestamp":1760058919171,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Comis\u00e3o Coordenadora da Regi\u00e3o Centro\u201d (Portugal)","award":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"]}]},{"name":"FCT-Portugal","award":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"]}]},{"name":"National Funds by FCT-Portugal","award":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000013","007491","ROTEIRO\/0031\/2013-PINFRA\/22161\/2016","SFRH\/BD\/06028\/2020","SFRH\/BD\/148695\/2019","UID\/Multi\/00709"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Hypertension is a highly prevalent medical condition that occurs when blood pressure is too high, which greatly increases the risk of developing other cardiovascular diseases and is generally associated with higher rates of morbidity and mortality. Due to the silent\/asymptomatic nature of hypertension, although the methods currently available to diagnose it are easy, they generally do not allow for an early diagnosis and an efficient prognosis to avoid irreversible damage in the medium or long term. In fact, an early diagnosis of hypertension would be crucial to decrease hypertension-associated mortality. Since metabolomics using NMR can provide a global measurement of various serum metabolites, it is very suitable for detecting novel biomarkers. We therefore analyzed serum metabolomic profiles among normotensive and hypertensive elderly individuals by NMR and identified new potential biomarkers for hypertension and associated diseases. We found higher levels of acetate, formate, and glycerol, and lower levels of glutamine, glycine, and sarcosine in individuals with hypertension. Therefore, these metabolites could be used for early diagnosis of hypertension to avoid comorbidities derived from hypertension and associated mortality.<\/jats:p>","DOI":"10.3390\/molecules30102145","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T09:26:48Z","timestamp":1747128408000},"page":"2145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nuclear Magnetic Resonance Analysis Seeking for Metabolic Markers of Hypertension in Human Serum"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1480-9279","authenticated-orcid":false,"given":"Adriana","family":"Sousa","sequence":"first","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Faculty of Health Sciences, University of Beira Interior (UBI), Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"given":"N\u00e1dia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Faculty of Health Sciences, University of Beira Interior (UBI), Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-081X","authenticated-orcid":false,"given":"Ricardo","family":"Conde","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"},{"name":"Center for Cooperative Research in Biosciences (CIC-BioGUNE), Bizkaia Science and Technology Park, 48160 Derio, Spain"}]},{"given":"Elisabete","family":"Morais","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1887-5027","authenticated-orcid":false,"given":"Ana Paula","family":"Amaral","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9878-3290","authenticated-orcid":false,"given":"Nieves","family":"Embade","sequence":"additional","affiliation":[{"name":"Center for Cooperative Research in Biosciences (CIC-BioGUNE), Bizkaia Science and Technology Park, 48160 Derio, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8748-4105","authenticated-orcid":false,"given":"Oscar","family":"Millet","sequence":"additional","affiliation":[{"name":"Center for Cooperative Research in Biosciences (CIC-BioGUNE), Bizkaia Science and Technology Park, 48160 Derio, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3492-5725","authenticated-orcid":false,"given":"Ignacio","family":"Verde","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS-UBI), University of Beira Interior (UBI), 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Faculty of Health Sciences, University of Beira Interior (UBI), Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2023). Global Report on Hypertension: The Race Against a Silent Killer, World Health Organization."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Louca, P., Nogal, A., Moskal, A., Goulding, N.J., Shipley, M.J., Alkis, T., Lindbohm, J.V., Hu, J., Kifer, D., and Wang, N. (2022). Cross-sectional blood metabolite markers of hypertension: A multicohort analysis of 44,306 Individuals from the Consortium of Metabolomics Studies. Metabolites, 12.","DOI":"10.3390\/metabo12070601"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ameta, K., Gupta, A., Kumar, S., Sethi, R., Kumar, D., and Mahdi, A.A. (2017). Essential hypertension: A filtered serum based metabolomics study. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-02289-9"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/iub.1885","article-title":"Metabolomic study for essential hypertension patients based on dried blood spot mass spectrometry approach","volume":"70","author":"Bai","year":"2018","journal-title":"IUBMB Life"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s11306-018-1408-y","article-title":"Metabolomic characterization of hypertension and dyslipidemia","volume":"14","author":"Ke","year":"2018","journal-title":"Metabolomics"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1161\/CIRCRESAHA.118.311342","article-title":"Omics of blood pressure and hypertension","volume":"122","author":"Arnett","year":"2018","journal-title":"Circ. Res."},{"key":"ref_7","unstructured":"Islam, M.S. (2017). Metabolomics, lipidomics and pharmacometabolomics of human hypertension. Hypertension: From Basic Research to Clinical Practice, Springer International Publishing."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Touyz, R.M., and Schiffrin, E.L. (2017). Urine metabolomics in hypertension research. Hypertension: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-6625-7"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11906-018-0877-8","article-title":"An Overview of metabolic phenotyping in blood pressure research","volume":"20","author":"Tzoulaki","year":"2018","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1186\/s12967-023-04176-6","article-title":"Alterations of gut microbiota are associated with blood pressure: A cross-sectional clinical trial in Northwestern China","volume":"21","author":"Lv","year":"2023","journal-title":"J. Transl. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1161\/HYPERTENSIONAHA.115.05315","article-title":"Gut dysbiosis is linked to hypertension","volume":"65","author":"Yang","year":"2015","journal-title":"Hypertension"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Li, J., Zhao, F., Wang, Y., Chen, J., Tao, J., Tian, G., Wu, S., Liu, W., Cui, Q., and Geng, B. (2017). Gut microbiota dysbiosis contributes to the development of hypertension. Microbiome, 5.","DOI":"10.1186\/s40168-016-0222-x"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/nature06882","article-title":"Human metabolic phenotype diversity and its association with diet and blood pressure","volume":"453","author":"Holmes","year":"2008","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1161\/HYPERTENSIONAHA.113.01166","article-title":"Metabolomics and incident hypertension among blacks","volume":"62","author":"Zheng","year":"2013","journal-title":"Hypertension"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Hao, Y., Wang, Y., Xi, L., Li, G., Zhao, F., Qi, Y., Liu, J., and Zhao, D. (2016). A nested case-control study of association between metabolome and hypertension risk. Biomed Res. Int., 2016.","DOI":"10.1155\/2016\/7646979"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Krzemi\u0144ska, J., Wronka, M., M\u0142ynarska, E., Franczyk, B., and Rysz, J. (2022). Arterial hypertension-oxidative stress and inflammation. Antioxidants, 11.","DOI":"10.3390\/antiox11010172"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1161\/HYPERTENSIONAHA.116.07292","article-title":"Identification of serum metabolites associated with incident hypertension in the european prospective investigation into cancer and nutrition\u2013potsdam study","volume":"68","author":"Dietrich","year":"2016","journal-title":"Hypertension"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.aca.2014.11.009","article-title":"Reconstruction and analysis of correlation networks based on GC-MS metabolomics data for young hypertensive men","volume":"854","author":"Wang","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1097\/HJH.0000000000002040","article-title":"Central systolic pressure and a nonessential amino acid metabolomics profile: The African Prospective study on the Early Detection and Identification of Cardiovascular disease and Hypertension","volume":"37","author":"Mels","year":"2019","journal-title":"J. Hypertens."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.amjmed.2005.09.010","article-title":"Glucose metabolism in patients with essential hypertension","volume":"119","author":"Ruilope","year":"2006","journal-title":"Am. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/HJH.0000000000003051","article-title":"Comprehensive biomarker profiling of hypertension in 36985 Finnish individuals","volume":"40","author":"Palmu","year":"2022","journal-title":"J. Hypertens."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1136\/pgmj.2006.048371","article-title":"Blood pressure and ageing","volume":"83","author":"Pinto","year":"2007","journal-title":"Postgrad. Med. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1007\/s11906-012-0260-0","article-title":"Hypertension in postmenopausal women","volume":"14","author":"Lima","year":"2012","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_24","first-page":"427","article-title":"Prevalence of cardiovascular risk factors and other comorbidities in patients with hypertension in Portuguese primary health care populations: The PRECISE study","volume":"38","author":"Lima","year":"2019","journal-title":"Rev. Port. Cardiol. (Engl. Ed.)"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1093\/eurheartj\/ehac395","article-title":"Hypertension management in patients with cardiovascular comorbidities","volume":"44","author":"Lauder","year":"2022","journal-title":"Eur. Heart J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.repc.2018.10.012","article-title":"Sociodemographic disparities in hypertension prevalence: Results from the first Portuguese National Health Examination Survey","volume":"38","author":"Rodrigues","year":"2019","journal-title":"Rev. Port. Cardiol. (Engl. Ed.)"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Yang, M., Yu, Z., Deng, S., Chen, X., Chen, L., Guo, Z., Zheng, H., Chen, L., Cai, D., and Wen, B. (2016). A targeted metabolomics mrm-ms study on identifying potential hypertension biomarkers in human plasma and evaluating acupuncture effects. Sci. Rep., 6.","DOI":"10.1038\/srep25871"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.cca.2018.08.006","article-title":"Identification of essential hypertension biomarkers in human urine by non-targeted metabolomics based on UPLC-Q-TOF\/MS","volume":"486","author":"Zhao","year":"2018","journal-title":"Clin. Chim. Acta"},{"key":"ref_29","first-page":"3673","article-title":"Study on plasmatic metabolomics of Uygur patients with essential hypertension based on nuclear magnetic resonance technique","volume":"18","author":"Zhong","year":"2014","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1007\/s00216-011-4880-z","article-title":"An ultrasonication-assisted extraction and derivatization protocol for GC\/TOFMS-based metabolite profiling","volume":"400","author":"Liu","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e181271","DOI":"10.1001\/jamanetworkopen.2018.1271","article-title":"Association of body mass index with blood pressure among 1.7 million chinese adults","volume":"1","author":"Linderman","year":"2018","journal-title":"JAMA Netw. Open"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jash.2014.11.007","article-title":"Use of metabolomics to elucidate the metabolic perturbation associated with hypertension in a black South African male cohort: The SABPA study","volume":"9","author":"Lindeque","year":"2015","journal-title":"J. Am. Soc. Hypertens."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"R\u00edos-Covi\u00e1n, D., Ruas-Madiedo, P., Margolles, A., Gueimonde, M., de Los Reyes-Gavil\u00e1n, C.G., and Salazar, N. (2016). Intestinal short chain fatty acids and their link with diet and human health. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00185"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s44161-022-00204-8","article-title":"Gut microbial metabolites lower blood pressure in patients with hypertension","volume":"2","author":"Jama","year":"2023","journal-title":"Nat. Cardiovasc. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1161\/CIRCULATIONAHA.116.024545","article-title":"High-fiber diet and acetate supplementation change the gut microbiota and prevent the development of hypertension and heart failure in hypertensive mice","volume":"135","author":"Marques","year":"2017","journal-title":"Circulation"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1515\/cclm-2012-0552","article-title":"Formate: An essential metabolite, a biomarker, or more?","volume":"51","author":"Lamarre","year":"2013","journal-title":"Clin Chem Lab Med"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1186\/s12933-024-02363-3","article-title":"MetSCORE: A molecular metric to evaluate the risk of metabolic syndrome based on serum NMR metabolomics","volume":"23","author":"Conde","year":"2024","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Oliveira, N., Sousa, A., Amaral, A., Gra\u00e7a, G., and Verde, I. (2023). Searching for metabolic markers of stroke in human plasma via NMR analysis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242216173"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3732","DOI":"10.2337\/dc13-0800","article-title":"Glycerol and fatty acids in serum predict the development of hyperglycemia and type 2 diabetes in Finnish men","volume":"36","author":"Mahendran","year":"2013","journal-title":"Diabetes Care"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12012-010-9096-5","article-title":"Glutathione-related antioxidant defense system in elderly patients treated for hypertension","volume":"11","author":"Rybka","year":"2011","journal-title":"Cardiovasc. Toxicol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lin, C., Sun, Z., Mei, Z., Zeng, H., Zhao, M., Hu, J., Xia, M., Huang, T., Wang, C., and Gao, X. (2022). The causal associations of circulating amino acids with blood pressure: A Mendelian randomization study. BMC Med., 20.","DOI":"10.1186\/s12916-022-02612-w"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1161\/HYPERTENSIONAHA.123.22649","article-title":"Interplay between plasma glycine and branched-chain amino acids contributes to the development of hypertension and coronary heart disease","volume":"81","author":"Dziedzic","year":"2024","journal-title":"Hypertension"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1139\/cjpp-2012-0341","article-title":"Oral supplementation with glycine reduces oxidative stress in patients with metabolic syndrome, improving their systolic blood pressure","volume":"91","author":"Cruz","year":"2013","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e002621","DOI":"10.1161\/JAHA.115.002621","article-title":"Plasma glycine and risk of acute myocardial infarction in patients with suspected stable angina pectoris","volume":"5","author":"Ding","year":"2015","journal-title":"J. Am. Heart Assoc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1007\/s00125-018-4599-x","article-title":"Metabolomics insights into early type 2 diabetes pathogenesis and detection in individuals with normal fasting glucose","volume":"61","author":"Merino","year":"2018","journal-title":"Diabetologia"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s00726-023-03236-x","article-title":"Accurately quantified plasma free glycine concentration as a biomarker in patients with acute ischemic stroke","volume":"55","author":"Wang","year":"2023","journal-title":"Amino Acids"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Durante, W. (2019). The emerging role of L-glutamine in cardiovascular health and disease. Nutrients, 11.","DOI":"10.3390\/nu11092092"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Yang, L., Xu, L., Li, J., Wang, H., Sun, J., Yu, Z., Zhao, X., Zhao, M., and Xi, B. (2022). The association of dietary glutamine supplementation with the development of high salt-induced hypertension in rats. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1011739"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1161\/CIRCULATIONAHA.111.067827","article-title":"Metabolite profiling identifies pathways associated with metabolic risk in humans","volume":"125","author":"Cheng","year":"2012","journal-title":"Circulation"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Go\u00efta, Y., Chao de la Barca, J.M., Ke\u00efta, A., Diarra, M.B., Demb\u00e9l\u00e9, K.C., Chabrun, F., Dram\u00e9, B.S.I., Kassogu\u00e9, Y., Diakit\u00e9, M., and Mirebeau-Prunier, D. (2020). Sexual dimorphism of metabolomic profile in arterial hypertension. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-64329-1"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"L810","DOI":"10.1152\/ajplung.00167.2020","article-title":"Protein biomarkers and risk scores in pulmonary arterial hypertension associated with ventricular septal defect: Integration of multi-omics and validation","volume":"319","author":"Yuan","year":"2020","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e97000","DOI":"10.5812\/jkums.97000","article-title":"Serum and urine levels of sarcosine in benign prostatic hyperplasia and newly diagnosed prostate cancer patients","volume":"24","author":"Yousefi","year":"2020","journal-title":"J. Kermanshah Univ. Med. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1038\/nature07762","article-title":"Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression","volume":"457","author":"Sreekumar","year":"2009","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1176\/ajp.139.9.1136","article-title":"The global deterioration scale for assessment of primary degenerative dementia","volume":"139","author":"Reisberg","year":"1982","journal-title":"Am. J. Psychiatry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1212\/01.wnl.0000434309.85312.19","article-title":"A brief cognitive test battery to differentiate Alzheimer\u2019s disease and frontotemporal dementia","volume":"55","author":"Mathuranath","year":"2000","journal-title":"Neurology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"781","DOI":"10.17219\/acem\/68975","article-title":"Validation of the Portuguese version of Addenbrooke\u2019s Cognitive Examination III in mild cognitive impairment and dementia","volume":"27","author":"Peixoto","year":"2018","journal-title":"Adv. Clin. Exp. Med. Off. Organ. Wroc. Med. Univ."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Bizkarguenaga, M., Bruzzone, C., Gil-Redondo, R., SanJuan, I., Martin-Ruiz, I., Barriales, D., Palacios, A., Pasco, S.T., Gonz\u00e1lez-Valle, B., and La\u00edn, A. (2022). Uneven metabolic and lipidomic profiles in recovered COVID-19 patients as investigated by plasma NMR metabolomics. NMR Biomed., 35.","DOI":"10.1002\/nbm.4637"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/10\/2145\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:31:52Z","timestamp":1760031112000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/10\/2145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,13]]},"references-count":57,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["molecules30102145"],"URL":"https:\/\/doi.org\/10.3390\/molecules30102145","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2025,5,13]]}}}