{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:03:27Z","timestamp":1774321407893,"version":"3.50.1"},"reference-count":109,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study investigated the treatment of unsterilized, undiluted, and unfiltered liquid digestate in a large-scale photobioreactor over a period of 33 weeks using a consortium of microalgae and bacteria. The generated biomass was analyzed for a wide spectrum of value-added compounds. The impact of organic loading rates (OLR) on the microbial culture was determined, and the influence of the biomass storage method on its qualitative composition was also analyzed. The experiment showed optimal growth of microalgae at OLR = 0.1 gCOD\/L\/day (where COD is Chemical Oxygen Demand), while a higher OLR value led to culture destabilization. Microglena sp., an algae not commonly applied for digestate treatment, showed low tolerance to changes in process conditions (OLR increase) but high readaptation potential when the OLR was lowered to its initial value. Significant changes in the microbial community were observed during the treatment. In Phases 1 and 2, Desmodesmus subspicatus and Actinomycetota phylum dominated in the community, while in Phase 3, Microglena sp. and Firmicutes were the most abundant. Total nitrogen, orthophosphates, and soluble COD were reduced by 89\u201399%. The biomass storage method had a notable impact on the content of lipids, fatty acids, and pigments. The protein amount was 32.75\u201333.59% of total solids (TS), while total lipid content was 15.76\u201319.00% TS, with stearic and palmitic acid being dominant. The effect of the storage regime on the potential biomass valorization was also discussed.<\/jats:p>","DOI":"10.3390\/molecules30132778","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T11:37:51Z","timestamp":1751283471000},"page":"2778","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous Liquid Digestate Treatment and High-Value Microalgal Biomass Production: Influence of Post-Harvest Storage on Biochemical Profiles"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0554-0578","authenticated-orcid":false,"given":"Ewelina","family":"Sobolewska","sequence":"first","affiliation":[{"name":"Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, W\u00f3lcza\u0144ska 171\/173, 90-530 Lodz, Poland"},{"name":"Interdisciplinary Doctoral School, Lodz University of Technology, \u017beromskiego 116, 90-924 Lodz, Poland"}]},{"given":"Micha\u0142","family":"Komar","sequence":"additional","affiliation":[{"name":"Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, W\u00f3lcza\u0144ska 171\/173, 90-530 Lodz, Poland"},{"name":"Interdisciplinary Doctoral School, Lodz University of Technology, \u017beromskiego 116, 90-924 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8867-3327","authenticated-orcid":false,"given":"Sebastian","family":"Borowski","sequence":"additional","affiliation":[{"name":"Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, W\u00f3lcza\u0144ska 171\/173, 90-530 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7722-9132","authenticated-orcid":false,"given":"Paulina","family":"Nowicka-Krawczyk","sequence":"additional","affiliation":[{"name":"Department of Algology and Mycology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12\/16, 90-237 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-6345","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Portugal","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"TERRA\u2014Associate Laboratory for Sustainable Land Use and Ecosystem Services, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"FitoLab\u2014Laboratory for Phytopathology, Instituto Pedro Nunes, Rua Pedro Nunes, 3030-199 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1748-6364","authenticated-orcid":false,"given":"Nuno","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4798-9381","authenticated-orcid":false,"given":"Mariana F. G.","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1607-6001","authenticated-orcid":false,"given":"Berk","family":"Aksoy","sequence":"additional","affiliation":[{"name":"Coimbra Collection of Algae (ACOI), Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5244-221X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Cotas","sequence":"additional","affiliation":[{"name":"Marine Resources, Conservation and Technology, Marine Algae Lab, Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6819-0619","authenticated-orcid":false,"given":"Leonel","family":"Pereira","sequence":"additional","affiliation":[{"name":"TERRA\u2014Associate Laboratory for Sustainable Land Use and Ecosystem Services, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"Marine Resources, Conservation and Technology, Marine Algae Lab, Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"114670","DOI":"10.1016\/j.rser.2024.114670","article-title":"Public Acceptance of Biomass for Bioenergy: The Need for Feedstock Differentiation and Communicating a Waste Utilization Frame","volume":"202","author":"Goedegebure","year":"2024","journal-title":"Renew. 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