{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:28:38Z","timestamp":1760059718807,"version":"build-2065373602"},"reference-count":100,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UID\/50006\/2023"],"award-info":[{"award-number":["UID\/50006\/2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Floridoside (2-O-D-glycerol-\u03b1-D-galactopyranoside) is a natural product typically found in red algae. It serves as the algae\u2019s carbon reserve and is produced as a protective response against osmotic and heat stress. Both floridoside and its acylated derivatives have been associated with modulating redox homeostasis and inflammatory responses. Therefore, we aimed to evaluate whether the newly synthesized floridoside phosphotriesters (1b\u20131d, 1f\u20131h) and acylated floridoside derivative (1e) can modulate the oxidative burst in stimulated human neutrophils. Synthetic strategies included the glycosylation of the thioglycoside donor with glycerol derivatives, having NIS\/TfOH as the promoter. Phosphorylation was achieved with POCl3 in the presence of pyridine. The compounds were analysed for their cytotoxicity, with 1b and 1h being cytotoxic at 50 \u03bcM, while the others showed no cytotoxicity in the tested concentrations. The detection of the neutrophils\u2019 oxidative burst was performed using multiple probes [luminol, aminophenyl fluorescein (APF), and Amplex Red (AR)] to evaluate reactive species levels. Compound 1e prevented the oxidative burst in activated human neutrophils (IC50 = 83 \u00b1 7 \u03bcM). All the other tested compounds were ineffective in inhibiting APF and AR oxidation under the present experimental conditions. These findings highlight the potential of floridoside-based derivatives as candidates for targeting inflammatory pathways.<\/jats:p>","DOI":"10.3390\/molecules30132850","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T09:57:39Z","timestamp":1751536659000},"page":"2850","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Floridoside Phosphotriester Derivatives: Synthesis and Inhibition of Human Neutrophils\u2019 Oxidative Burst"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0224-8045","authenticated-orcid":false,"given":"Lu\u00eds","family":"Pinheiro","sequence":"first","affiliation":[{"name":"LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Campus da Caparica, 2825-149 Caparica, Portugal"}]},{"given":"Catarina","family":"Cipriano","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Campus da Caparica, 2825-149 Caparica, Portugal"}]},{"given":"Filipe","family":"Santos","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Campus da Caparica, 2825-149 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0387-5411","authenticated-orcid":false,"given":"Patr\u00edcia","family":"M\u00e1ximo","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Campus da Caparica, 2825-149 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6424-0976","authenticated-orcid":false,"given":"Eduarda","family":"Fernandes","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9114-9967","authenticated-orcid":false,"given":"Marisa","family":"Freitas","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-8596","authenticated-orcid":false,"given":"Paula S.","family":"Branco","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, Campus da Caparica, 2825-149 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fischer, W. 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