{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:50:01Z","timestamp":1771037401929,"version":"3.50.1"},"reference-count":72,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["407015\/2023"],"award-info":[{"award-number":["407015\/2023"]}]},{"name":"National Council for Scientific and Technological Development (CNPq)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"National Council for Scientific and Technological Development (CNPq)","award":["407015\/2023"],"award-info":[{"award-number":["407015\/2023"]}]},{"name":"Foundation for Research and Technological Innovation Support of the State of Sergipe (FAPITEC\/SE)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Foundation for Research and Technological Innovation Support of the State of Sergipe (FAPITEC\/SE)","award":["407015\/2023"],"award-info":[{"award-number":["407015\/2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study explored the antioxidant and anticancer potential of extracts obtained from the mangaba, cambu\u00ed, and red propolis blend. The extracts were obtained using ultrasound-assisted pressurized fluid extraction (UAPFE) at 50 bar, 60 \u00b0C, and a flow rate of 2 mL\/min. Both sequential extraction with solvents of increasing polarity (propane followed by ethanol\/water) and one-step extraction were employed for 30 min. Extracts were characterized by ultra-high-resolution mass spectrometry, total phenolic content, antioxidant activity (via DPPH and FRAP assays), and cytotoxicity using the sulforhodamine B colorimetric method. Among the tested conditions, the sequential extraction with ethanol\/water (UAPFE-SE) yielded 16.2 \u00b1 3.0% (overall extraction yield), with high phenolic content (24.1 \u00b1 0.4 \u00b5g\/mg). Mass spectrometry revealed the presence of antiproliferative phenolics. The UAPFE-SE extract demonstrated moderate antioxidant activity, with FRAP values of 394.0 \u00b1 6.0 \u00b5g Fe2+\/mg and DPPH scavenging capacity of 28.5 \u00b1 0.3 \u00b5g Trolox equivalents\/mg. Additionally, it exhibited cytotoxic inhibition of 82.3 \u00b1 1.7% against lung carcinoma cells at a concentration of 100 \u03bcg\/mL. The results suggest that the antioxidant properties and cytotoxic effect against lung cancer cells in vitro warrant further investigation to assess therapeutic potential.<\/jats:p>","DOI":"10.3390\/molecules30193857","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T15:54:54Z","timestamp":1758642894000},"page":"3857","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ultrasound-Assisted Pressurized Fluid Extraction of Antioxidant and Anticancer Molecules from a Mangaba, Cambu\u00ed and Red Propolis Blend"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5621-8270","authenticated-orcid":false,"given":"Diego S. de","family":"Oliveira","sequence":"first","affiliation":[{"name":"Center for Study on Colloidal Systems (NUESC), Institute of Research and Technology and (ITP), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9396-9846","authenticated-orcid":false,"given":"Mar\u00edlia R.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Center for Study on Colloidal Systems (NUESC), Institute of Research and Technology and (ITP), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2866-2052","authenticated-orcid":false,"given":"Glenda A. da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Sergipe (UFS), S\u00e3o Crist\u00f3v\u00e3o 49100-000, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8954-6285","authenticated-orcid":false,"given":"Cristiane B.","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Sergipe (UFS), S\u00e3o Crist\u00f3v\u00e3o 49100-000, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4177-9335","authenticated-orcid":false,"given":"Ana Veruska C. da","family":"Silva","sequence":"additional","affiliation":[{"name":"Empresa Brasileira de Pesquisa Agropecu\u00e1ria (EMBRAPA), Av. Beira-Mar, 3.250, Aracaju 49025-040, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9789-4551","authenticated-orcid":false,"given":"Jhonattas de C.","family":"Carregosa","sequence":"additional","affiliation":[{"name":"Center for Study on Colloidal Systems (NUESC), Institute of Research and Technology and (ITP), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6815-0265","authenticated-orcid":false,"suffix":"Jr.","given":"Alberto","family":"Wisniewski","sequence":"additional","affiliation":[{"name":"Petroleum and Energy from Biomass Research Group (PEB), Department of Chemistry, Federal University of Sergipe (UFS), S\u00e3o Crist\u00f3v\u00e3o 49100-000, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-6596","authenticated-orcid":false,"given":"Maria Beatriz P. P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5239-9039","authenticated-orcid":false,"given":"Claudio","family":"Dariva","sequence":"additional","affiliation":[{"name":"Center for Study on Colloidal Systems (NUESC), Institute of Research and Technology and (ITP), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5772-9634","authenticated-orcid":false,"given":"Klebson S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Center for Study on Colloidal Systems (NUESC), Institute of Research and Technology and (ITP), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49032-490, SE, Brazil"},{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dias, A.L.B., de Aguiar, A.C., and Rostagno, M.A. (2021). Extraction of Natural Products Using Supercritical Fluids and Pressurized Liquids Assisted by Ultrasound: Current Status and Trends. Ultrason. Sonochem., 74.","DOI":"10.1016\/j.ultsonch.2021.105584"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Aldana-Mej\u00eda, J.A., Ribeiro, V.P., Meepagala, K.M., Bastos, J.K., and Ross, S.A. (2025). Bioactive Metabolites of Brazilian Red Propolis: Cytotoxic, Antimalarial, and Antimicrobial Properties. 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