{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:02:17Z","timestamp":1760241737661,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T00:00:00Z","timestamp":1536105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGRCFL\/71437\/2006"],"award-info":[{"award-number":["PTDC\/AGRCFL\/71437\/2006"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.<\/jats:p>","DOI":"10.3390\/mps1030034","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T02:55:07Z","timestamp":1536202507000},"page":"34","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots"],"prefix":"10.3390","volume":"1","author":[{"given":"Carla","family":"Ragonezi","sequence":"first","affiliation":[{"name":"Banco de Germoplasma ISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9640-8227","authenticated-orcid":false,"given":"Maria Amely","family":"Zavattieri","sequence":"additional","affiliation":[{"name":"Banco de Germoplasma ISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"},{"name":"Departamento de Biologia, P\u00f3lo da Mitra Apartado 94, 7002-554 \u00c9vora, Portugal"},{"name":"Instituto de Ci\u00eancias da Terra (ICT), Col\u00e9gio Lu\u00eds Ant\u00f3nio Verney, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/B978-0-12-407679-2.00003-X","article-title":"Polymicrobial multi-functional approach for enhancement crop productivity","volume":"82","author":"Reddy","year":"2013","journal-title":"Adv. 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