{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T21:55:31Z","timestamp":1769550931504,"version":"3.49.0"},"reference-count":5,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T00:00:00Z","timestamp":1546819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGR-CFL\/71437\/2006"],"award-info":[{"award-number":["PTDC\/AGR-CFL\/71437\/2006"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released\/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.<\/jats:p>","DOI":"10.3390\/mps2010005","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T03:22:31Z","timestamp":1547090551000},"page":"5","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds"],"prefix":"10.3390","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8543-4574","authenticated-orcid":false,"given":"M\u00e1rio Rui da Costa Bas\u00edlio e","family":"Castro","sequence":"first","affiliation":[{"name":"Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM). Col\u00e9gio da Mitra, Ap. 94, 7002-554 \u00c9vora, Portugal"}]},{"given":"Carla","family":"Ragonezi","sequence":"additional","affiliation":[{"name":"Banco de GermoplasmaISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]},{"given":"Paulo Guilherme Leandro de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, P\u00f3lo da Mitra Apartado 94, 7002-554 \u00c9vora, Portugal"},{"name":"Research Centre in Biodiversity and Genetic Resources \u00c9vora (CIBIO-UE), Ap. 94, 7002-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9640-8227","authenticated-orcid":false,"given":"Maria Amely","family":"Zavattieri","sequence":"additional","affiliation":[{"name":"Banco de GermoplasmaISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"},{"name":"Departamento de Biologia, P\u00f3lo da Mitra Apartado 94, 7002-554 \u00c9vora, Portugal"},{"name":"Instituto de Ci\u00eancias da Terra (ICT), Col\u00e9gio Lu\u00eds Ant\u00f3nio Verney, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/forestry\/76.5.579","article-title":"Sustained in vitro root development obtained in Pinus pinea inoculated with ectomycorrhizal fungi","volume":"76","author":"Oliveira","year":"2003","journal-title":"Forestry"},{"key":"ref_2","unstructured":"Ragonezi, C.A. (2013). In Vitro Plantlet Regeneration from Mature Zygotic Embryos of Pinus pinea L.: Overcoming the Rooting Problems. [Ph.D. Thesis, University of \u00c9vora]. Available online: http:\/\/hdl.handle.net\/10174\/16017."},{"key":"ref_3","unstructured":"Castro, M.R.B., Ragonezi Gomes Lopes, C.A., de Oliveira, P.G.L., and Zavattieri, M.A. (2012). Sistema e M\u00e9todo de Cultura de Plantas In Vitro Para An\u00e1lise de Metabolitos Libertados pelo Sistema Radicular. (n\u00ba 105239), Portuguese Patent, Available online: https:\/\/tinyurl.com\/y77axlnf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1080\/17429145.2013.831489","article-title":"O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arrhizus symbiosis established in vitro","volume":"9","author":"Ragonezi","year":"2014","journal-title":"J. Plant Interact."},{"key":"ref_5","unstructured":"Hartmann, H.T., Kester, D.E., Davis, F.T., and Geneve, R.L. (2002). Plant Propagation: Principles and Practices, Prentice-Hall Inc.. [7th ed.]."}],"container-title":["Methods and Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/1\/5\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:24:06Z","timestamp":1760185446000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/1\/5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,7]]},"references-count":5,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,3]]}},"alternative-id":["mps2010005"],"URL":"https:\/\/doi.org\/10.3390\/mps2010005","relation":{},"ISSN":["2409-9279"],"issn-type":[{"value":"2409-9279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,7]]}}}