{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:45:00Z","timestamp":1778600700094,"version":"3.51.4"},"reference-count":6,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T00:00:00Z","timestamp":1555027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.<\/jats:p>","DOI":"10.3390\/mps2020029","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T11:15:58Z","timestamp":1555326958000},"page":"29","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line"],"prefix":"10.3390","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-6337","authenticated-orcid":false,"given":"Carla","family":"Luis","sequence":"first","affiliation":[{"name":"School of Health, Polytechnic Institute of Porto, 4200 Porto, Portugal"},{"name":"Institute of Research and Innovation in Health (i3S), Porto University, 4200 Porto, Portugal"},{"name":"Faculty of Medicine, Porto University, 4200 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6155-5083","authenticated-orcid":false,"given":"Yuselis","family":"Casta\u00f1o-Guerrero","sequence":"additional","affiliation":[{"name":"BioMark-CEB\/ISEP, School of Engineering, Polytechnic Institute of Porto, 4200 Porto, Portugal"}]},{"given":"Raquel","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Research and Innovation in Health (i3S), Porto University, 4200 Porto, Portugal"},{"name":"Faculty of Medicine, Porto University, 4200 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9936-7336","authenticated-orcid":false,"given":"Goreti","family":"Sales","sequence":"additional","affiliation":[{"name":"BioMark-CEB\/ISEP, School of Engineering, Polytechnic Institute of Porto, 4200 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-3984","authenticated-orcid":false,"given":"R\u00faben","family":"Fernandes","sequence":"additional","affiliation":[{"name":"School of Health, Polytechnic Institute of Porto, 4200 Porto, Portugal"},{"name":"Institute of Research and Innovation in Health (i3S), Porto University, 4200 Porto, Portugal"},{"name":"Faculty of Medicine, Porto University, 4200 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1200\/JCO.1993.11.6.1132","article-title":"High-dose chemotherapy and autologous bone marrow support as consolidation after standard-dose adjuvant therapy for high-risk primary breast cancer","volume":"11","author":"Peters","year":"1993","journal-title":"J. Clin. Oncol."},{"key":"ref_2","first-page":"1","article-title":"DNA-Encoded Library Technology: A Brief Guide to Its Evolution and Impact on Drug Discovery","volume":"50","author":"Goodnow","year":"2017","journal-title":"Annu. Rep. Med. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1387-2656(05)11004-7","article-title":"Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction","volume":"11","author":"Berridge","year":"2005","journal-title":"Biotechnol. Annu. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/bjc.1987.190","article-title":"A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity","volume":"56","author":"Twentyman","year":"1987","journal-title":"Br. J. Cancer"},{"key":"ref_6","unstructured":"Riss, T. (2014). Is Your MTT Assay Really the Best Choice?. Promega Corp."}],"container-title":["Methods and Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/2\/29\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:45:05Z","timestamp":1760186705000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/2\/29"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,12]]},"references-count":6,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["mps2020029"],"URL":"https:\/\/doi.org\/10.3390\/mps2020029","relation":{},"ISSN":["2409-9279"],"issn-type":[{"value":"2409-9279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,12]]}}}