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The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pristine graphene nanoplatelets (GNP) on biofilm development through the representative marine bacteria Cobetia marina and to investigate the antibacterial mechanisms of action of this material. For this purpose, a flow cytometric analysis was performed and a GNP\/polydimethylsiloxane (PDMS) surface containing 5 wt% GNP (G5\/PDMS) was produced, characterized, and assessed regarding its biofilm mitigation potential over 42 days in controlled hydrodynamic conditions that mimic marine environments. Flow cytometry revealed membrane damage, greater metabolic activity, and endogenous reactive oxygen species (ROS) production by C. marina when exposed to GNP 5% (w\/v) for 24 h. In addition, C. marina biofilms formed on G5\/PDMS showed consistently lower cell count and thickness (up to 43% reductions) than PDMS. Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS. Overall, the GNP-based surface inhibited C. marina biofilm development, showing promising potential as a marine antifouling coating.<\/jats:p>","DOI":"10.3390\/nano13030381","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T03:45:16Z","timestamp":1674099916000},"page":"381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3148-8948","authenticated-orcid":false,"given":"Francisca","family":"Sousa-Cardoso","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-9362","authenticated-orcid":false,"given":"Rita","family":"Teixeira-Santos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Ana Francisca","family":"Campos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1358-1380","authenticated-orcid":false,"given":"Marta","family":"Lima","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8992-1097","authenticated-orcid":false,"given":"Luciana C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9015-1237","authenticated-orcid":false,"given":"Ol\u00edvia S. 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P.","family":"Soares","sequence":"additional","affiliation":[{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"LSRE-LCM\u2014Laboratory of Separation and Reaction Engineering\u2014Laboratory of Catalysis and Materials, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe J.","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,18]]},"reference":[{"key":"ref_1","first-page":"10","article-title":"Marine Biofouling: A Sticky Problem","volume":"49","author":"Callow","year":"2002","journal-title":"Biologist"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3389\/fmars.2018.00126","article-title":"Marine Biofilms: A Successful Microbial Strategy with Economic Implications","volume":"5","year":"2018","journal-title":"Front. Mar. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/08927014.2010.542809","article-title":"Economic Impact of Biofouling on a Naval Surface Ship","volume":"27","author":"Schultz","year":"2011","journal-title":"Biofouling"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Edyvean, R. (2010). Consequences of Fouling on Shipping. Biofouling, Wiley-Blackwell.","DOI":"10.1002\/9781444315462.ch15"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"126428","DOI":"10.1016\/j.jclepro.2021.126428","article-title":"Greenhouse Gas Emissions Reduction Potential by Using Antifouling Coatings in a Maritime Transport Industry","volume":"295","author":"Farkas","year":"2021","journal-title":"J. Clean Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1080\/08927010903046268","article-title":"The Role of Containerships as Transfer Mechanisms of Marine Biofouling Species","volume":"25","author":"Davidson","year":"2009","journal-title":"Biofouling"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.marenvres.2014.06.006","article-title":"Marine Fouling Invasions in Ports of Patagonia (Argentina) with Implications for Legislation and Monitoring Programs","volume":"99","author":"Schwindt","year":"2014","journal-title":"Mar. Environ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1080\/08927014.2019.1640214","article-title":"Biofouling in Marine Aquaculture: A Review of Recent Research and Developments","volume":"35","author":"Bannister","year":"2019","journal-title":"Biofouling"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s11998-014-9651-2","article-title":"Effects of Biofouling Development on Drag Forces of Hull Coatings for Ocean-Going Ships: A Review","volume":"12","author":"Lindholdt","year":"2015","journal-title":"J. Coat. Technol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"503","DOI":"10.5194\/os-6-503-2010","article-title":"Biofouling Protection for Marine Environmental Sensors","volume":"6","author":"Delauney","year":"2010","journal-title":"Ocean. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7536","DOI":"10.1039\/D0TA02000A","article-title":"Slippery Liquid-Infused Porous Surfaces (SLIPSs): A Perfect Solution to Both Marine Fouling and Corrosion?","volume":"8","author":"Deng","year":"2020","journal-title":"J. Mater. Chem. A Mater."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11998-012-9456-0","article-title":"A Brief Review of Environmentally Benign Antifouling and Foul-Release Coatings for Marine Applications","volume":"10","author":"Buskens","year":"2013","journal-title":"J. Coat. Technol. Res."},{"key":"ref_13","first-page":"1","article-title":"Marine Biofilms Constitute a Bank of Hidden Microbial Diversity and Functional Potential","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s10126-007-9001-9","article-title":"Marine Biofilms as Mediators of Colonization by Marine Macroorganisms: Implications for Antifouling and Aquaculture","volume":"9","author":"Qian","year":"2007","journal-title":"Mar. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1111\/1462-2920.12186","article-title":"Marine Biofilms on Artificial Surfaces: Structure and Dynamics","volume":"15","author":"Salta","year":"2013","journal-title":"Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lima, M., Gomes, L.C., Teixeira-Santos, R., Romeu, M.J., Valcarcel, J., V\u00e1zquez, J.A., Cerqueira, M.A., Pastrana, L., Bourbon, A.I., and de Jong, E.D. (2022). Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232314647"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1574-6968.1996.tb08408.x","article-title":"Attachment of Bacteria to Model Solid Surfaces: Oligo(Ethylene Glycol) Surfaces Inhibit Bacterial Attachment","volume":"142","author":"Ista","year":"1996","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1128\/AEM.70.7.4151-4157.2004","article-title":"Effect of Substratum Surface Chemistry and Surface Energy on Attachment of Marine Bacteria and Algal Spores","volume":"70","author":"Ista","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"31014","DOI":"10.1116\/6.0000240","article-title":"Microfluidic Accumulation Assay to Quantify the Attachment of the Marine Bacterium Cobetia Marina on Fouling-Release Coatings","volume":"15","author":"Schwarze","year":"2020","journal-title":"Biointerphases"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Akuzov, D., Franca, L., Grunwald, I., and Vladkova, T. (2018). Sharply Reduced Biofilm Formation from Cobetia marina and in Black Sea Water on Modified Siloxane Coatings. Coatings, 8.","DOI":"10.3390\/coatings8040136"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Abouelkheir, S.S., Abdelghany, E.A., Sabry, S.A., and Ghozlan, H.A. (2021). Biofilm Formation by Marine Cobetia Marina Alex and Pseudoalteromonas Spp: Development and Detection of Quorum Sensing N-Acyl Homoserine Lactones (AHLs) Molecules. J. Mar. Sci., 3.","DOI":"10.30564\/jms.v3i3.3397"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1007\/BF01569982","article-title":"Deleya Marina as a Model Organism for Studies of Bacterial Colonization and Biofilm Formation","volume":"15","author":"Shea","year":"1995","journal-title":"J. Ind. Microbiol"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3390\/life5021218","article-title":"Role of Cyanobacterial Exopolysaccharides in Phototrophic Biofilms and in Complex Microbial Mats","volume":"5","author":"Rossi","year":"2015","journal-title":"Life"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jobab.2021.11.003","article-title":"Effects of Chitin Nanocrystals on Coverage of Coating Layers and Water Retention of Coating Color","volume":"7","author":"Gao","year":"2022","journal-title":"J. Bioresour. Bioprod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.jobab.2021.02.009","article-title":"Packaging and Degradability Properties of Polyvinyl Alcohol\/Gelatin Nanocomposite Films Filled Water Hyacinth Cellulose Nanocrystals","volume":"6","author":"Oyeoka","year":"2021","journal-title":"J. Bioresour. Bioprod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12951-015-0137-x","article-title":"A Nanomolecular Approach to Decrease Adhesion of Biofouling-Producing Bacteria to Graphene-Coated Material","volume":"13","author":"Parra","year":"2015","journal-title":"J. Nanobiotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"771098","DOI":"10.3389\/fnano.2021.771098","article-title":"Nanocoating Is a New Way for Biofouling Prevention","volume":"3","author":"Kumar","year":"2021","journal-title":"Front. Nanotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1080\/08927010701784391","article-title":"Attachment Point Theory Revisited: The Fouling Response to a Microtextured Matrix","volume":"24","author":"Scardino","year":"2008","journal-title":"Biofouling"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1038\/ncomms1251","article-title":"Trends in the Development of Environmentally Friendly Fouling-Resistant Marine Coatings","volume":"2","author":"Callow","year":"2011","journal-title":"Nat. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sousa-Cardoso, F., Teixeira-Santos, R., and Mergulh\u00e3o, F.J.M. (2022). Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review. Antibiotics, 11.","DOI":"10.3390\/antibiotics11081102"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Romeu, M.J., Lima, M., Gomes, L.C., de Jong, E.D., Morais, J., Vasconcelos, V., Pereira, M.F.R., Soares, O.S.G.P., Sjollema, J., and Mergulh\u00e3o, F.J. (2022). The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite. Polymers, 14.","DOI":"10.3390\/polym14204410"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Romeu, M.J., Gomes, L.C., Sousa-Cardoso, F., Morais, J., Vasconcelos, V., Whitehead, K.A., Pereira, M.F.R., Soares, O.S.G.P., and Mergulh\u00e3o, F.J. (2022). How Do Graphene Composite Surfaces Affect the Development and Structure of Marine Cyanobacterial Biofilms?. Coatings, 12.","DOI":"10.3390\/coatings12111775"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s41061-019-0235-6","article-title":"Current Review on Synthesis, Composites and Multifunctional Properties of Graphene","volume":"377","author":"Sattar","year":"2019","journal-title":"Top Curr. Chem."},{"key":"ref_34","first-page":"223","article-title":"Emerging Trends in Graphene Carbon Based Polymer Nanocomposites and Applications","volume":"32","author":"Idumah","year":"2016","journal-title":"Rev. Chem. Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5992","DOI":"10.1007\/s10853-018-03244-3","article-title":"A Review of the Mechanical and Thermal Properties of Graphene and Its Hybrid Polymer Nanocomposites for Structural Applications","volume":"54","author":"Kumar","year":"2019","journal-title":"J. Mater. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"102001","DOI":"10.1016\/j.isci.2020.102001","article-title":"Antimicrobial and Anti-Adhesive Properties of Carbon Nanotube-Based Surfaces for Medical Applications: A Systematic Review","volume":"24","author":"Gomes","year":"2021","journal-title":"iScience"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kumar, P., Huo, P., Zhang, R., and Liu, B. (2019). Antibacterial Properties of Graphene-Based Nanomaterials. Nanomaterials, 9.","DOI":"10.3390\/nano9050737"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1016\/j.jmrt.2021.01.093","article-title":"Mechanism and Factors Influence of Graphene-Based Nanomaterials Antimicrobial Activities and Application in Dentistry","volume":"11","author":"Radhi","year":"2021","journal-title":"J. Mater. Res. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2000076","DOI":"10.1002\/adsu.202000076","article-title":"Toward the Application of Graphene for Combating Marine Biofouling","volume":"5","author":"Jin","year":"2021","journal-title":"Adv. Sustain. Syst."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1078\/0723-2020-00113","article-title":"Proposal of Cobetia marina Gen. Nov., Comb. Nov., within the Family Halomonadaceae, to Include the Species Halomonas Marina","volume":"25","author":"Arahal","year":"2002","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1099\/00207713-48-4-1205","article-title":"Pseudoalteromonas tunicata Sp. Nov., a Bacterium That Produces Antifouling Agents","volume":"48","author":"James","year":"1998","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s10295-009-0681-1","article-title":"Bacterial Assay for the Rapid Assessment of Antifouling and Fouling Release Properties of Coatings and Materials","volume":"37","author":"Bruin","year":"2010","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Mortimer, M., Petersen, E., Buchholz, B., and Holden, P. (2016). Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation. Nanomaterials, 6.","DOI":"10.3390\/nano6100181"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1111\/jam.12394","article-title":"Effect of Pulsed Light on Structural and Physiological Properties of Listeria innocua and Escherichia coli","volume":"116","author":"Kramer","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.3389\/fmicb.2018.02486","article-title":"Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis","volume":"9","author":"Li","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0022-1759(92)90008-H","article-title":"A Microplate Assay for the Detection of Oxidative Products Using 2\u2032,7\u2032-Dichlorofluorescin-Diacetate","volume":"156","author":"Rosenkranz","year":"1992","journal-title":"J. Immunol. Methods"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1002\/jbm.a.35277","article-title":"Escherichia coli Adhesion, Biofilm Development and Antibiotic Susceptibility on Biomedical Materials","volume":"103","author":"Gomes","year":"2015","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"106609","DOI":"10.1016\/j.porgcoat.2021.106609","article-title":"Oriented Immobilization of Pep19-2.5 on Antifouling Brushes Suppresses the Development of Staphylococcus aureus Biofilms","volume":"163","author":"Vorobii","year":"2022","journal-title":"Prog. Org. Coat."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Faria, S.I., Teixeira-Santos, R., Romeu, M.J., Morais, J., Vasconcelos, V., and Mergulh\u00e3o, F.J. (2020). The Relative Importance of Shear Forces and Surface Hydrophobicity on Biofilm Formation by Coccoid Cyanobacteria. Polymers, 12.","DOI":"10.5194\/biofilms9-33"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"E1697","DOI":"10.1002\/pc.25125","article-title":"Incorporation of Carbon Nanotubes in Polydimethylsiloxane to Control Escherichia coli Adhesion","volume":"40","author":"Vagos","year":"2019","journal-title":"Polym. Compos."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Oliveira, I.M., Gomes, M., Gomes, L.C., Pereira, M.F.R., Soares, O.S.G.P., and Mergulh\u00e3o, F.J. (2022). Performance of Graphene\/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms. Nanomaterials, 12.","DOI":"10.3390\/nano12030355"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Gomes, M., Gomes, L.C., Teixeira-Santos, R., Pereira, M.F.R., Soares, O.S.G.P., and Mergulh\u00e3o, F.J. (2021). Optimizing CNT Loading in Antimicrobial Composites for Urinary Tract Application. Appl. Sci., 11.","DOI":"10.3390\/app11094038"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"110976","DOI":"10.1016\/j.colsurfb.2020.110976","article-title":"The Potential Advantages of Using a Poly(HPMA) Brush in Urinary Catheters: Effects on Biofilm Cells and Architecture","volume":"191","author":"Alves","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"7455","DOI":"10.1016\/j.polymer.2007.10.038","article-title":"Fabrication of Super-Hydrophobic Film from PMMA with Intrinsic Water Contact Angle below 90\u00b0","volume":"48","author":"Ma","year":"2007","journal-title":"Polymer"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"van Oss, C.J. (2006). Interfacial Forces in Aqueous Media, CRC Press.","DOI":"10.1201\/9781420015768"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1128\/aem.48.5.980-983.1984","article-title":"Measurement of the Surface Free Energy of Bacterial Cell Surfaces and Its Relevance for Adhesion","volume":"48","author":"Busscher","year":"1984","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.msec.2005.10.045","article-title":"Quantification of the Adhesion Free Energy between Bacteria and Hydrophobic and Hydrophilic Substrata","volume":"26","author":"Bayoudh","year":"2006","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Faria, S.I., Teixeira-Santos, R., Romeu, M.J., Morais, J., de Jong, E., Sjollema, J., Vasconcelos, V., and Mergulh\u00e3o, F.J. (2021). Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms. Microorganisms, 9.","DOI":"10.3390\/microorganisms9051102"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4411","DOI":"10.1111\/1462-2920.14807","article-title":"Biofilm Formation Behaviour of Marine Filamentous Cyanobacterial Strains in Controlled Hydrodynamic Conditions","volume":"21","author":"Romeu","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"6280","DOI":"10.1128\/AEM.69.10.6280-6287.2003","article-title":"Comparison of Velocity Profiles for Different Flow Chamber Designs Used in Studies of Microbial Adhesion to Surfaces","volume":"69","author":"Bakker","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.etap.2017.12.001","article-title":"Antifouling Processes and Toxicity Effects of Antifouling Paints on Marine Environment. A Review","volume":"57","author":"Amara","year":"2018","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/femsec\/fiab052","article-title":"The Association between Initial Adhesion and Cyanobacterial Biofilm Development","volume":"97","author":"Faria","year":"2021","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"111566","DOI":"10.1016\/j.envres.2021.111566","article-title":"Quantitative Proteomic Analysis of Marine Biofilms Formed by Filamentous Cyanobacterium","volume":"201","author":"Romeu","year":"2021","journal-title":"Environ. Res."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Faria, S.I., Gomes, L.C., Teixeira-Santos, R., Morais, J., Vasconcelos, V., and Mergulh\u00e3o, F.J.M. (2021). Developing New Marine Antifouling Surfaces: Learning from Single-Strain Laboratory Tests. Coatings, 11.","DOI":"10.3390\/coatings11010090"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Dobretsov, S. (2010). Marine Biofilms. Biofouling, Wiley-Blackwell.","DOI":"10.1002\/9781444315462.ch9"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1109\/TSMC.1979.4310076","article-title":"Threshold Selection Method From Gray-Level Histograms","volume":"SMC-9","author":"Otsu","year":"1979","journal-title":"IEEE Trans. Syst. Man Cybern."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3389\/fbioe.2020.00465","article-title":"Antimicrobial Mechanisms and Effectiveness of Graphene and Graphene-Functionalized Biomaterials. A Scope Review","volume":"8","author":"Mohammed","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Dawan, J., and Ahn, J. (2022). Bacterial Stress Responses as Potential Targets in Overcoming Antibiotic Resistance. Microorganisms, 10.","DOI":"10.3390\/microorganisms10071385"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3389\/fmicb.2017.00459","article-title":"Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method That Minimizes ROS Measurement Artifacts with Fluorescent Dyes","volume":"8","author":"McBee","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.mimet.2018.02.009","article-title":"A Multi-Step Approach for Testing Non-Toxic Amphiphilic Antifouling Coatings against Marine Microfouling at Different Levels of Biological Complexity","volume":"146","author":"Zecher","year":"2018","journal-title":"J. Microbiol. Methods"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"110738","DOI":"10.1016\/j.colsurfb.2019.110738","article-title":"Inhibition of Biofilm Formation by Rough Shark Skin-Patterned Surfaces","volume":"186","author":"Chien","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"102336","DOI":"10.1016\/j.cis.2020.102336","article-title":"Hydrodynamics and Surface Properties Influence Biofilm Proliferation","volume":"288","author":"Krsmanovic","year":"2021","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Zhou, W., Apkarian, R., Wang, Z.L., and Joy, D. (2006). Fundamentals of Scanning Electron Microscopy (SEM). Scanning Microscopy for Nanotechnology, Springer.","DOI":"10.1007\/978-0-387-39620-0_1"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"275714","DOI":"10.1088\/0957-4484\/22\/27\/275714","article-title":"Comparing Carbon Nanotubes and Graphene Nanoplatelets as Reinforcements in Polyamide 12 Composites","volume":"22","author":"Chatterjee","year":"2011","journal-title":"Nanotechnology"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Staneva, A.D., Dimitrov, D.K., Gospodinova, D.N., and Vladkova, T.G. (2021). Antibiofouling Activity of Graphene Materials and Graphene-Based Antimicrobial Coatings. Microorganisms, 9.","DOI":"10.3390\/microorganisms9091839"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.cis.2016.08.002","article-title":"Antimicrobial Graphene Family Materials: Progress, Advances, Hopes and Fears","volume":"236","author":"Lukowiak","year":"2016","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1080\/1040841X.2021.1962802","article-title":"Water in Bacterial Biofilms: Pores and Channels, Storage and Transport Functions","volume":"48","author":"Quan","year":"2022","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.1099\/mic.0.26211-0","article-title":"A Three-Dimensional, Stochastic Simulation of Biofilm Growth and Transport-Related Factors That Affect Structure","volume":"149","author":"Chang","year":"2003","journal-title":"Microbiology"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/13\/3\/381\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:09:03Z","timestamp":1760119743000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/13\/3\/381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,18]]},"references-count":78,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["nano13030381"],"URL":"https:\/\/doi.org\/10.3390\/nano13030381","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,18]]}}}